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pShuttle-CMV-GFP U6promoter

Plasmid

#128049

Purpose

Adenoviral shuttle vector for shRNA expression from U6 promoter with GFP marker

Depositor

Oskar Laur

Type

Empty backbone

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pIRES2-EGFP-p53 tethered

Plasmid

#49245

Purpose

expresses two human WT p53s tethered together and also expresses EGFP in mammalian cells

Depositor

Dylan Taatjes

Article

Lin et al Aging Cell. 2013 Oct;12(5):863-72. doi:

Insert

p53-p53 (TP53 Human)

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 27, 2013

Availability

Academic Institutions and Nonprofits only

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pLVX-RNaseHI-NES-EGFP

Plasmid

#196701

Purpose

Plasmid for stable expression of wild type bacterial RNase HI tagged with NES and EGFP that can be used to specifically degrade the DNA-RNA hybrids in the cytoplasm.

Depositor

Karlene Cimprich

Article

Crossley et al Nature. 2023 Jan;613(7942):187-194. doi:

Insert

bacterial RNaseHI

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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pCMMP-EnvA-IRES-GFP

Plasmid

#32206

Depositor

Edward Callaway

Article

Wickersham et al Neuron. 2007 Mar 1. 53(5):639-47.

Insert

EnvARGCD

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-Ef1a-DIO_EGFP-GFE3

Plasmid

#79871

Purpose

For generating adeno-associated virus to express GFE3 under the EF1a promoter in a 'Cre-on' dependent manner.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-GFE3

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau E14

Plasmid

#46907

Purpose

expresses EGFP tagged Tau E14 in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau E14 (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

all 14 S/P or T/P amino acid residues (T111, T153…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pHAGE-FLAG-APEX2-GFP

Plasmid

#175757

Purpose

Lentiviral vector expressing N-terminally tagged APEX2-FLAG-GFP (Control for APEX experiments)

Depositor

Wade Harper

Article

Eapen et al Elife. 2021 Sep 29;10. pii: 72328. doi:

Insert

GFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau AP P301L

Plasmid

#46906

Purpose

expresses EGFP tagged Tau AP P301L in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

Tau AP P301L (MAPT Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

P301L AND all 14 S/P or T/P amino acid residues (…

Promoter

CMV

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pHRm-GFP(1-10)-VP64-NLS

Plasmid

#70228

Purpose

Expresses GFP(1-10)-VP64-NLS in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP(1-10)-VP64-NLS

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO-EGFP-WPRE-pA

Plasmid

#37085

Purpose

Expresses EGFP in cells lacking Cre (Cre-Off)

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

EGFP

Use

AAV and Cre/Lox; Cre-off

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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pCW57.1 N-term GFP tTA

Plasmid

#107551

Purpose

All-in-one dox-repressible expression of cDNA with optional N-term GFP tag.

Depositor

David Sabatini

Type

Empty backbone

Use

Lentiviral

Tags

GFP (optional)

Expression

Mammalian

Mutation

Promoter

Available since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGCDNsam-Foxa1-IRES-GFP

Plasmid

#33007

Depositor

Atsushi Suzuki

Article

Sekiya et al Nature. 2011 Jun 29;475(7356):390-3. doi

Insert

forkhead box A1 (Foxa1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

March 6, 2012

Availability

Academic Institutions and Nonprofits only

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pLVET-HA-K-RasG12V-IRES-GFP

Plasmid

#107140

Purpose

A bicistronic lentiviral mammalian expression vector for HA-KRasG12V(4B) and GFP

Depositor

Aki Manninen

Article

Zhang et al Oncogene. 2017 Oct 12;36(41):5681-5694.

Insert

HA-tagged Kras4B(G12V) and GFP (KRAS Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hEF-1alpha

Available since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin- soCoChR-GFP

Plasmid

#107708

Purpose

A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Human Synapsin (hSynapsin) promoter

Depositor

Edward Boyden

Article

Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

Has Service

AAV9

Insert

soCoChR-GFP

Use

AAV

Tags

EGFP and KA2

Expression

Mammalian

Mutation

Promoter

hSynapsin

Available since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pFUGW-FerH-ffLuc2-eGFP

Plasmid

#71393

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

Depositor

Glenn Merlino

Article

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

FerH-ffLuc2-eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

FerH

Available since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA4TO-24xGCN4_v1-P2A-sfGFP

Plasmid

#61057

Purpose

sfGFP and the 24xGCN4_v1 peptide array are expressed from the same transcript, separated by a P2A site

Depositor

Ron Vale

Article

Tanenbaum et al Cell. 2014 Oct 8. pii: S0092-8674(14)012

Insert

24xGCN4_v1 and sfGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2014

Availability

Academic Institutions and Nonprofits only
pFA6a-link-yoEGFP-SpHis5

Plasmid

#44836

Purpose

Yeast tagging vector to create gene fusion with yeast optimized EGFP with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 9, 2013

Availability

Academic Institutions and Nonprofits only

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pLPCX-Cx43-IRES-GFP

Plasmid

#65433

Purpose

Mammalian expression of Cx43 and EGFP seperately driven by the ECMV IRES. For transfection or retroviral production

Depositor

Trond Aasen

Article

Salat-Canela et al Cell Commun Signal. 2014 May 8;12:31. do

Insert

Cx43 (GJA1 Human)

Use

Retroviral

Tags

IRES-GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2015

Availability

Academic Institutions and Nonprofits only

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pBS-ACTB-C200-GFPfr1

Plasmid

#169798

Purpose

THe donor vector for the ACTB gene locus.

Depositor

Natsuko Chiba

Article

Yoshino et al Sci Rep. 2019 Feb 7;9(1):1644. doi: 10.1

Insert

Donor sequence to ACTB gene for HR

Use

Hr donor vector for human actb gene.

Tags

Expression

Mutation

Partial sequence of GFP is inserted before the st…

Promoter

Available since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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1179_pAAV-U6-BbsI-gRNA-CB-EmGFP

Plasmid

#89060

Purpose

AAV-gRNA cloning vector with GFP reporter

Depositor

William Lagor

Article

Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

Insert

EmGFP

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Chicken beta actin with partial CMV enhancer

Available since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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