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CP1041-ABE8e-dimer

Plasmid

#138499

Purpose

A-to-G base editor

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

ecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1041)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA_DiCre_59.F2

Plasmid

#60207

Purpose

Expresses a fusion protein of FKBP12-linker peptide 2- Cre(19-59)

Depositor

Jean-Paul Herman

Article

Jullien et al Nucleic Acids Res. 2003 Nov 1;31(21):e13

Insert

Cre recombinase (CD207 Human, Synthetic, Bacteriophage P1)

Use

Tags

FKBP12 and Nuclear localisation signal

Expression

Mammalian

Mutation

amino acids 19-59 of the humanized version of Cre…

Promoter

CMV

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pEF-DEST51 HA-PNRC1

Plasmid

#123294

Purpose

Mammalian expression of PNRC1 N-HA

Depositor

Giovanni Tonon

Article

Gaviraghi et al EMBO J. 2018 Dec 3;37(23). pii: embj.201

Insert

PNRC1 (PNRC1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBABEpuro-Flag-ZO-2

Plasmid

#27420

Depositor

Marius Sudol

Article

Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

Insert

Zonula Occludens 2 (TJP2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
pBS U6 WASp RNAi

Plasmid

#11775

Depositor

Jack Strominger

Article

Krzewski et al J Cell Biol. 2006 Apr 10. 173(1):121-32.

Insert

WASp RNAi (WAS Human)

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEBTet-SNAP-FOP

Plasmid

#136796

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-FGFR

Use

Tags

FLAG-tag / His-tag

Expression

Mammalian

Mutation

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR_L1-BirA(R118G)-YFP-NLS-STOP-L2

Plasmid

#127360

Purpose

gateway entry vector for expressing nuclear BirA* (R118G)-YFP under a promoter of choice

Depositor

Dominique Bergmann

Article

Mair et al Elife. 2019 Sep 19;8. pii: 47864. doi: 1

Insert

BirA* (R118G mutant)

Use

Gateway entry vector

Tags

GS linker, NLS, V5, and YFP

Expression

Mutation

R118G

Promoter

no promoter

Available since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFPC1/GgVcl 1-851 A50I mutant

Plasmid

#46269

Depositor

Susan Craig

Article

Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

Insert

Vcl 1-851 A50I mutation (VCL Chicken)

Use

Tags

EGFP

Expression

Mammalian

Mutation

1-851 (Vh) head domain, A50I mutation

Promoter

CMV

Available since

July 11, 2013

Availability

Academic Institutions and Nonprofits only

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pKD280

Plasmid

#125071

Purpose

Expression of SO_4387 under Ptac

Depositor

Jeffrey Tabor

Article

Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

Inserts

SO_4387 (SO_4387 )
lacI (lacI )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

S286L- Please see depositor comments below

Promoter

Available since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX6p-Meis1a

Plasmid

#78144

Purpose

bacterial expression of GST-Meis1a

Depositor

Francesco Blasi

Article

Dardaei et al Proc Natl Acad Sci U S A. 2014 Mar 11;11

Insert

Meis1a (Meis1 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

June 10, 2016

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-24

Plasmid

#172750

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-24; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-24

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-9

Plasmid

#172742

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-9; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-9

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT-DEST EVC2-TEV-YFP-Flag

Plasmid

#41004

Depositor

Rajat Rohatgi

Article

Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

Insert

Ellis van Creveld syndrome 2 (EVC2 Mouse)

Use

Tags

TEV-YFP-Flag

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 15, 2012

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Cerulean-WW-NES

Plasmid

#84629

Purpose

Encodes N-terminal (PAABD) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targeted

Depositor

Jin Zhang

Article

Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do

Insert

Cerulean-WW-NES

Use

Tags

6xHis, Cerulean, Nuclear export signal (NES), T7 …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pHis-hPim1

Plasmid

#100722

Purpose

Expression of human Pim1 kinase domain in E.coli

Depositor

Byung Il Lee

Article

Lee et al PLoS One. 2013 Jul 31;8(7):e70358. doi:

Insert

Pim1 (PIM1 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Residues 29-313 of isoform 2

Promoter

T7 promotor

Available since

Oct. 31, 2017

Availability

Academic Institutions and Nonprofits only

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pSFFV-dNme2Cas9

Plasmid

#193190

Purpose

Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

dNme2Cas9

Use

Tags

NLS and NLS-3xHA-NLS

Expression

Mammalian

Mutation

H588A, D16A

Promoter

SFFV

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-BIRC3-SU

Plasmid

#160699

Purpose

Expresses GFP-BIRC3 with the short 3'UTR in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP-BIRC3 (BIRC3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-nCASphi_E9t_V2_U6_AtPDS3_gRNA10

Plasmid

#197965

Purpose

T-DNA binary vector to express pco-nCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-nCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6-26 and pUBQ10

Available since

May 26, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-GFP-BIRC3-NU

Plasmid

#160698

Purpose

Expresses GFP-BIRC3 without the endogenous 3'UTR in mammalian cells

Depositor

Christine Mayr

Article

Lee et al Mol Cell. 2019 May 16;74(4):701-712.e9.

Insert

GFP-BIRC3 (BIRC3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 17, 2020

Availability

Academic Institutions and Nonprofits only

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pOPS0379

Plasmid

#115510

Purpose

Suitable for experiments in environments where there is no antibiotic selection present. Symbiosis-induced nifH promoter from Rhizobium leguminosarum biovar vicia 3841 (Rlv3841pNifH) expressing sfGFP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Rlv3841pNifH(pOGG082) sfGFP (pOGG037) and T-pharma (pOGG003) assembled in pOGG026

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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