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Plasmid#207792PurposeDonor template for Puro-2A-moxGFP insertion into the N-terminus of the GOLGA2 locus for Golgi visualization. To be co-transfected with sgRNA plasmid px330-PITCh-GOLGA2 Addgene #207791DepositorArticleInsertGOLGA2 Homology Arms flanking a Puro-moxGFP Cassette (GOLGA2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pACUH-GFP11x7-mCherry-α-tubulin
Plasmid#70218PurposeExpresses GFP11x7-mCherry-α-tubulin in insect cells. 5 a. a. linker lengths.DepositorInsertGFP11x7-mCherry-α-tubulin
UseTagsGFP11x7 and mCherryExpressionInsectMutationPromoterAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLPCX2-MT1-MMP-eGFP
Plasmid#89819PurposeExpress in MT1-MMP mammalian cellsDepositorInsertMT1-MMP (MMP14 Human)
UseRetroviralTagseGFPExpressionMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMXs GFP-LC3-RFP
Plasmid#117413PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic flux without any drug resistance.DepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
AICSDP-117:POLR2A-mEGFP
Plasmid#164500PurposeHomology arms and mEGFP-linker sequence sequence for N-terminus tagging of human POLR2ADepositorInsertPOLR2A Homology Arms with mEGFP-linker (POLR2A Human)
UseCRISPR; Donor templateTagsmEGFP-linkerExpressionMammalianMutationhomology arms contain point mutations to disrupt …PromoterAvailable sinceFeb. 17, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
p23-puro-EGFP-FBL
Plasmid#179235Purposeoverexpression in human cellsDepositorInsertFBL (FBL Human)
UseTagsEGFPExpressionMammalianMutationPromoterEF-1alphaAvailable sinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10-P2A-GFP
Plasmid#162509PurposeExpress ABE7.10-P2A-GFP by CMV promoter in mammalian cellsDepositorInsertABE7.10-P2A-GFP
UseCRISPRTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSicoR-eIF1a-GFP-RPL10a
Plasmid#159890PurposeExpresses GFP-RPL10a for ribosomal immunoprecipitationDepositorInsertRPL10a (RPL10A Human)
UseLentiviralTagsGFPExpressionMutationPromotereIF1aAvailable sinceSept. 22, 2020AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-YAP (S94A)
Plasmid#174169PurposeLentiviral vector to constitutively express TEAD-binding mutant YAP under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationSerine 94 mutated to AlaninePromoterEFSAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCVL SFFV d14GFP Donor
Plasmid#31475DepositorInsertSFFV d14GFP
UseLentiviralTagsExpressionMutationDeleted 14 amino acids on the c terminus of eGFPPromoterAvailable sinceAug. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
BII-tdT-inv-GFP
Plasmid#133393PurposePiggybac vector for bidirectional promoter reporter assayDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHR RPS27A-3xFlag_IRES-GFP
Plasmid#198390PurposeRPS27A (ubiquitin/ribosomal protein eS31 fusion) expressionDepositorInsertRPS27A (RPS27A Human)
UseLentiviralTags3xFlagExpressionMammalianMutationPromoterEF1AAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO GFP HSPA8
Plasmid#19487DepositorInsertHSPA8 (HSPA8 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceOct. 6, 2008AvailabilityAcademic Institutions and Nonprofits only -
LentiU6-LacZ-GFP-Puro (BB)
Plasmid#170459PurposeFor cloning gRNA blockDepositorInserthU6-BB-LacZ and EF1A-EGFP-2A-Puro
UseLentiviralTagsExpressionMammalianMutationPromoterhU6 for gRNA and EF1A for EGFP-2A-PuroAvailable sinceAug. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGCDNsam-Hnf4a-IRES-GFP
Plasmid#33006DepositorInserthepatic nuclear factor 4 alpha (Hnf4a Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceMarch 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCOTS-pyl-GFP(35TAG)
Plasmid#92047PurposeThis is a S. elongatus (PCC7942) cyanobacterial plasmid that encodes the pylRS orthogonal translation system. In addition it encodes for a GFP reporter with TAG mutation at site 35.DepositorInsertsEGFP
Pyrrolysyl tRNA(cua)
Pyrrolysyl tRNA synthetase (methanosarcina mazei)
UseReplicative expression plasmid for cyanobacteria …TagsHistagExpressionMutationTyrosine in position 35 of the GFP was mutated f…PromoterLeuP (native S. elongatus promoter), PpsbII, and …Available sinceOct. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNL4-3 envFS eGFP NoStarts
Plasmid#101342PurposeAll ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACGDepositorInsertNL4-3
UseLentiviralTagsExpressionMutationNo methonines in GagPromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV sgRNA-Notch1 #1 GFP
Plasmid#106950PurposeLentivirus encoding sgRNA targeting murine Notch1. Includes GFP marker.DepositorInsertgRNA targeting Notch1 (Notch1 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBI-p53 R175H/EGFP
Plasmid#16544DepositorInsertp53 (TP53 Human)
UseTagsExpressionMammalianMutationR175HPromoterAvailable sinceJune 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
pEGFP/golgin-160/3DE
Plasmid#180631PurposeExpression of caspase-resistant golgin-160DepositorInsertgolgin-160/3DE (GOLGA3 Human)
UseTagsEGFPExpressionMammalianMutationD59E, D139E, D311EPromoterAvailable sinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only