We narrowed to 892 results for: pgem

1. Dat - EGFP - Tol2

   Plasmid

   #85418

   Purpose

   for making transgenic zebrafish expressing GFP under the control of cis-regulatory elements of the dopamine transporter (dat) gene

   Depositor

   Marc Ekker

   Article

   Xi et al Dev Dyn. 2011 Nov;240(11):2539-47. doi:

   Insert

   dopamine transporter (slc6a3 Zebrafish)
   

   Use

   Zebrafish transgenesis

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   dat

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pISH-Gap43

   Plasmid

   #74336

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Gap43. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Hirota et al Proc Natl Acad Sci U S A. 2004 Jun 8;101

   Insert

   growth associated protein 43 (Gap43 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLI-N-sandwich-loxP(K13)

   Plasmid

   #85793

   Purpose

   P. falciparum integration plasmid for N-terminal gene fusions (double 2xFKBP) and placing loxP sites using SLI

   Depositor

   Tobias Spielmann

   Article

   Birnbaum et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

   Insert

   Kelch13
   

   Use

   Cre/Lox; P. falciparum genomic integration

   Tags

   double 2xFKBP, GFP

   Expression

   Mutation

   Promoter

   none

   Available since

   March 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pISH-Emx1-SR

   Plasmid

   #105214

   Purpose

   to generate a riboprobe for in situ hybridazation

   Depositor

   Peter Mombaerts

   Article

   Saraiva et al Sci Rep. 2015 Dec 16;5:18178. doi: 10.10

   Insert

   Emx1 (Emx1 Mouse)
   

   Use

   To ganerate riboprobe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMK-Cas9-gate

   Plasmid

   #113743

   Purpose

   Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving aminoglycoside phosphotransferase for G418 selection in plants.

   Depositor

   Magdalena Bezanilla

   Article

   Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

   Insert

   Cas9
   

   Use

   CRISPR; Gateway destination vector

   Tags

   Expression

   Mutation

   Promoter

   Z. Mays ubiquitin promoter, 35S promoter

   Available since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pFH6

   Plasmid

   #86555

   Purpose

   Subcloning of any sgRNA via BbsI sites. Note that there is an improved version of this plasmid (Addgene 105866) that results in up to 10x greater efficiency

   Depositor

   Andreas Weber

   Article

   Hahn et al Front Plant Sci. 2017 Jan 24;8:39. doi:

   Insert

   U6-26p::sgRNA scaffold
   

   Use

   CRISPR; Subcloning

   Tags

   Expression

   Mutation

   Promoter

   Arabidopsis U6-26 promoter

   Available since

   May 16, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pISH-Adrb2

   Plasmid

   #105199

   Purpose

   to generate a riboprobe for in situ hybridazation

   Depositor

   Peter Mombaerts

   Article

   Omura et al Mol Cell Neurosci. 2014 Jan;58:1-10. doi

   Insert

   Adrb2 (Adrb2 Mouse)
   

   Use

   To ganerate riboprobe

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pISH-Hopx

   Plasmid

   #74347

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Hopx. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

   Insert

   HOP homeobox (Hopx Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 9, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. Gcm2TEASY

   Plasmid

   #41031

   Depositor

   Nancy Manley

   Article

   Gordon et al Mech Dev. 2001 May;103(1-2):141-3.

   Insert

   Gcm2 in situ probe (Gcm2 Mouse)
   

   Use

   In situ probe

   Tags

   Expression

   Mutation

   contains nucleotides 584-1510 of GenBank accessio…

   Promoter

   T7 sense; SP6 antisense

   Available since

   Oct. 29, 2012

   Availability

   Academic Institutions and Nonprofits only

10. Hox A13b

    Plasmid

    #36468

    Depositor

    Robert Ho

    Article

    Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

    Insert

    hoxA13b (hoxa11b Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Mutation

    F232C

    Promoter

    T7

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

11. Hox D13a

    Plasmid

    #36467

    Depositor

    Robert Ho

    Article

    Ahn et al Dev Biol. 2008 Oct 1;322(1):220-33. Epub

    Insert

    hoxa11b (hoxa11b Zebrafish)
    

    Use

    Riboprobe synthesis

    Tags

    Expression

    Mutation

    F63I, P75Q and M133I

    Promoter

    T7

    Available since

    Sept. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pFL-TK-AID3'UTR-MUT

    Plasmid

    #19987

    Depositor

    Nina Papavasiliou

    Article

    Teng et al Immunity. 2008 May . 28(5):621-9.

    Insert

    AID 3' UTR (Aicda Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCINTBeta1

    Plasmid

    #14067

    Depositor

    Richard Hynes

    Article

    Tamkun et al Cell. 1986 Jul 18. 46(2):271-82.

    Insert

    Chicken Beta 1 Integrin (ITGB1 Chicken)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PstI-BglI fragment was blunt end cloned and EcoRI…

    Promoter

    Available since

    March 2, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pR-Avr

    Plasmid

    #22650

    Depositor

    Lynn Hudson

    Article

    Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

    Insert

    Myelin Transcription Factor 1 (Myt1 Rat)
    

    Use

    Cloning and rna synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

15. pR-Pst

    Plasmid

    #22648

    Depositor

    Lynn Hudson

    Article

    Kim et al J Neurosci Res. 1997 Oct 15. 50(2):272-9

    Insert

    Myelin Transcription Factor 1 (Myt1 Rat)
    

    Use

    Cloning and rna synthesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 1, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pFL-TK-AID3'UTR-DEL

    Plasmid

    #19988

    Depositor

    Nina Papavasiliou

    Article

    Teng et al Immunity. 2008 May . 28(5):621-9.

    Insert

    AID 3' UTR (Aicda Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Deletion

    Promoter

    Available since

    Jan. 9, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. HBV 1.3-mer X-null replicon

    Plasmid

    #65461

    Purpose

    containing 1.3 units of the X-null HBV genome (subtype ayw)

    Depositor

    Wang-Shick Ryu

    Article

    Hussain et al J Gen Virol. 2009 Sep;90(Pt 9):2272-81.

    Insert

    1.3 units of the X-null HBV genome
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pλPR cI-Elysis

    Plasmid

    #176891

    Purpose

    Bacterial expression plasmids for production of E. coli bacterial ghost (BG) capsules

    Depositor

    James Inglese

    Article

    Dranchak et al Dis Model Mech. 2023 Mar 1;16(3):dmm0498

    Insert

    Lysis E gene from PhiX174 and lambda PR-cI857 regulatory system
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPR-HOT_tdTomato

    Plasmid

    #138567

    Purpose

    Universal Cleavable Donor Plasmid for tagging with tdTomato using NHEJ

    Depositor

    Hans Clevers

    Article

    Artegiani et al Nat Cell Biol. 2020 Mar;22(3):321-331. d

    Insert

    tdTomato
    

    Use

    Unspecified

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGal4x5-E4T-G210

    Plasmid

    #171077

    Purpose

    The resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permission

    Depositor

    Joan Conaway

    Article

    He et al Proc Natl Acad Sci U S A. 2021 Sep 28;11

    Insert

    Gal4-E4-Gless-cassette
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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