Skip to main content
Addgene
Showing: 721 - 740 of 3281 results

  1. pJG488

    Plasmid
    #91196
    Purpose
    WDV replicon T-DNA for gene targeting in wheat scutella, H840A double nickase (nTaCas9_H840A+gUbi8+gUbi1+donor)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    nTaCas9_H840A+gUbi8+gUbi1+donor
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91196-plasmid-map-sequence-id-182459

    Enlarge

  2. pMOD_C2519

    Plasmid
    #91088
    Purpose
    Module C, Promoter: OsU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    OsU3
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91088-plasmid-map-sequence-id-182053

    Enlarge

  3. pMOD_B2000

    Plasmid
    #91059
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALEN_1), Gene: TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALEN_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    none (begins with P2A to be fused to TALEN_1)
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91059-plasmid-map-sequence-id-181870

    Enlarge

  4. pMOD_B2403

    Plasmid
    #91071
    Purpose
    Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers , Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with ribozyme spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    CmYLCV
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91071-plasmid-map-sequence-id-181880

    Enlarge

  7. pMOD_B3200

    Plasmid
    #91079
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALE-VPR_1), Gene: TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VPR_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    none (begins with P2A to be fused to TALE-VPR_1)
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91079-plasmid-map-sequence-id-182042

    Enlarge

  8. pMOD_B3400

    Plasmid
    #91080
    Purpose
    Module B, Promoter: none (begins with P2A to be fused to TALE-VP64_1), Gene: TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: HSP
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VP64_2 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    none (begins with P2A to be fused to TALE-VP64_1)
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    91080-plasmid-map-sequence-id-182043

    Enlarge

  9. pMOD_A3101

    Plasmid
    #91048
    Purpose
    Module A, Promoter: 35S, Gene: TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VPR_ 2)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VPR_1 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    35S
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91048-plasmid-map-sequence-id-181838

    Enlarge

  10. pMOD_A3301

    Plasmid
    #91049
    Purpose
    Module A, Promoter: 35S, Gene: TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning, Terminator: none (ends with P2A to be fused to TALE-VP64_ 2)
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    TALE-VP64_1 backbone with Esp3I ccdb cassette for repeat cloning
    Use
    TALEN
    Tags
    Expression
    Mutation
    Promoter
    35S
    Available since
    July 10, 2017
    Availability
    Academic Institutions and Nonprofits only
    91049-plasmid-map-sequence-id-181839

    Enlarge

  15. pKb-TnpB2

    Plasmid
    #215334
    Purpose
    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site
    Depositor
    Kutubuddin Molla
    Article
    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10
    Insert
    Rice codon optimized ISDra2 TnpB
    Use
    Tags
    Not applicable
    Expression
    Plant
    Mutation
    Promoter
    Rice ubiquitin promoter for TnpB and maize ubiqui…
    Available since
    Oct. 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    215334-plasmid-map-sequence-id-447325

    Enlarge

  16. pKb-TnpB1

    Plasmid
    #215333
    Purpose
    ISDra2 expression driven by PolII promoter ; 20 bp guide RNA can be cloned in BsaI site
    Depositor
    Kutubuddin Molla
    Article
    Karmakar et al Plant Biotechnol J. 2024 Jun 28. doi: 10
    Insert
    Rice codon optimized ISDra2 TnpB
    Use
    Tags
    Not applicable
    Expression
    Plant
    Mutation
    Promoter
    Rice ubiquitin promoter for TnpB and OsU3 promote…
    Available since
    Sept. 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    215333-plasmid-map-sequence-id-446611

    Enlarge

  17. pMOD_C2200

    Plasmid
    #91082
    Purpose
    Module C, Promoter: none – to be combined with gRNA array in module B, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers (only works with pMOD_B2203), Terminator: 35S
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    none (will be fused to gRNA array in MODULE B)
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91082-plasmid-map-sequence-id-182046

    Enlarge

  18. pBiex PKC - Peptide 8 GFP RFP

    Plasmid
    #83564
    Purpose
    Expresses a GFP-RFP FRET sensor containing the catalytic domain of PKCalpha and a short peptide substrate derived from the EGF receptor.
    Depositor
    Sivaraj Sivaramakrishnan
    Article
    Sommese et al J Biol Chem. 2016 Aug 23. pii: jbc.M116.
    Insert
    PKC alpha (PRKCA Human)
    Use
    Tags
    10 nm ER/K Helix, FLAG purification tag, TEV Prot…
    Expression
    Bacterial and Insect
    Mutation
    Only catalytic domain of PKC (aa 335-672)
    Promoter
    T7 Promoter
    Available since
    Nov. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    83564-plasmid-map-sequence-id-166406

    Enlarge

  20. pMOD_C2515

    Plasmid
    #91083
    Purpose
    Module C, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Daniel Voytas
    Article
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    AtU6
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    91083-plasmid-map-sequence-id-182047

    Enlarge

Showing: 721 - 740 of 3281 results