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pCytERM-miRFP2-N1

Plasmid

#197173

Purpose

Expresses the fusion protein of CytERM-miRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

CytERM-miRFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pB2AR-miRFP718nano

Plasmid

#195751

Purpose

Beta-2-adrenergic receptor internally tagged with miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

B2AR-miRFP718nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pMyosin-miRFP718nano

Plasmid

#195748

Purpose

LC-Myosin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-LC-Myosin

Use

Tags

miRFP718nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pClathrin-miRFP718nano

Plasmid

#195747

Purpose

Clathrin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-clathrin light chain

Use

Tags

miRFP718nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP718nano

Plasmid

#195746

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-human alpha-tubulin

Use

Tags

miRFP718nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pH2B-miRFP718nano

Plasmid

#195750

Purpose

Histone 2B labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

H2B-miRFP718nano

Use

Tags

miRFP718nano

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRBx2

Plasmid

#193569

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and two FRB mutants (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Use

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pGas-miRFP718nano

Plasmid

#195752

Purpose

G protein alpha-subunit internally tagged with miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

G protein alpha-s-miRFP718nano

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pNC-RFP-N

Plasmid

#193412

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pNC-RFP-C

Plasmid

#193413

Purpose

Nimble cloning-compatible vector for protein subcellular localization in plants

Depositor

Pu Yan

Article

Yan et al Plant Commun. 2023 Mar 13;4(2):100471. d

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST_6xUAS:miRFP670-cetn4

Plasmid

#192357

Purpose

zfCETN4 tagged with miRFP670, expressed under the UAS promoter

Depositor

Darren Gilmour

Article

Moller et al Elife. 2022 Nov 18;11:e82094. doi: 10.75

Insert

CETN4 (cetn4 Zebrafish)

Use

Tags

miRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pA-RFP-rG2

Plasmid

#188969

Purpose

IPTG inducible mCherry with sgRNA

Depositor

Seung-Goo Lee

Article

Kim et al Nucleic Acids Res. 2022 Dec 9;50(22):131

Inserts

mCherry
sgRNA: agtccatgtaatcagcgtctactagt

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Ptrc

Available since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pKeratin-emiRFP2-N1

Plasmid

#178972

Purpose

Expresses the fusion protein of Keratin-emiRFP2 in mammalian cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

Keratin-emiRFP2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pSF11-wmiRFP2

Plasmid

#178969

Purpose

Expresses miRFP2 in worm cells

Depositor

Kiryl Piatkevich

Article

Babakhanova et al Protein Sci. 2022 Mar;31(3):728-751. doi

Insert

wmiRFP2

Use

Tags

no

Expression

Worm

Mutation

NO

Promoter

tag-168 promoter

Available since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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iRFP670-KASH2ext

Plasmid

#187022

Purpose

Control for inducible disruption of LINC Complex

Depositor

Jan Lammerding

Insert

KASH2ext

Use

Tags

iRFP670

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pCytERM-miRFP670nano3

Plasmid

#184674

Purpose

Signal anchor of rabbit cytochrome p450 and miRFP670nano3

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

CytERM-miRFP670nano3

Use

Tags

miRFP670nano3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pTS2355_Tier3(PB)-iRFP_PuroR

Plasmid

#169654

Purpose

Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a iRFP670 marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-iRFP670-p2A-PuroR-pA-3'ITR)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PRPBSA-driven iRFP670 and PuroR expression

Use

Tags

Expression

Mammalian

Mutation

Promoter

PRPBSA

Available since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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H2B-HT-emiRFP703

Plasmid

#183985

Purpose

Expresses fluorescent HaloTag7 fusion protein in mammalian cells with nuclear localization.

Depositor

Pablo Rivera-Fuentes

Article

Emmert et al Nat Chem. 2023 Oct;15(10):1415-1421. doi

Insert

H2B (H2BC11 Human)

Use

Tags

HaloTag, emiRFP703

Expression

Mammalian

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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cASPG1-mRFP

Plasmid

#181962

Purpose

ASPG1 protein tagged with mRFP, under control of TBA2 promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

ASPG1 (ASPG1 Mustard Weed)

Use

Tags

mRFP

Expression

Plant

Mutation

Promoter

TBA2 promoter

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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gSCPL35-mRFP

Plasmid

#181960

Purpose

SCPL35 protein tagged with mRFP, under control of native promoter

Depositor

George Haughn

Article

Lee et al Plant Direct. 2021 Nov 28;5(11):e360. do

Insert

SCPL35

Use

Tags

mRFP

Expression

Plant

Mutation

Promoter

native SCPL35

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

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