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Showing: 721 - 740 of 1327 results

  2. pcDNA5/FRT/TO/Flag-tGFP

    Plasmid
    #110375
    Purpose
    Mammalian expression of turboGFP with FLAG tag, Flp-In T-Rex system
    Depositor
    Sandra Martha Gomes Dias
    Insert
    turboGFP
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    110375-plasmid-map-sequence-id-213401

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  3. mT2A-tdTomato-FNF

    Plasmid
    #182340
    Purpose
    Recombineering cassette plasmid for T2A-tdTomato-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-tdTomato-FNF
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182340-plasmid-map-sequence-id-358215

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  4. pMOD4-mT2A-mutated_rpsL-BSD-F

    Plasmid
    #182338
    Purpose
    Template plasmid for PCR amplification of initial recombineering cassette
    Depositor
    Mario Capecchi
    Insert
    BSD
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    182338-plasmid-map-sequence-id-358191

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  5. F3 Neo* F3

    Plasmid
    #140235
    Purpose
    F3 (FRT variant)-flanked pPgk-pEM7-Neo*-bpA positive selection cassette
    Depositor
    Mario Capecchi
    Insert
    Neo*
    Use
    Mouse Targeting; Flp/ frt
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    Nov. 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    140235-plasmid-map-sequence-id-316186

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  6. mT2A-td-sfGFP-FNF

    Plasmid
    #182339
    Purpose
    Recombineering cassette plasmid for T2A-td-sfGFP-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-td-sfGFP-FNF
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182339-plasmid-map-sequence-id-358195

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  7. pRDA_852

    Plasmid
    #185527
    Purpose
    Lentiviral vector that enables Cre-mediated expression of two sgRNAs. Also encodes the fluorophore violet-excited GFP (Vex) as a marker of transduction.
    Depositor
    Arlene Sharpe
    Inserts
    First sgRNA cassette
    Second sgRNA cassette
    Use
    CRISPR, Cre/Lox, and Lentiviral ; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human U6 and Mouse U6
    Available since
    April 1, 2025
    Availability
    Academic Institutions and Nonprofits only
    185527-plasmid-map-sequence-id-366261

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  8. pTO_CHMP4B_LAP

    Plasmid
    #101850
    Purpose
    Tet-inducible expression of human CHMP4B with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration
    Depositor
    Daniel Gerlich
    Article
    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d
    Insert
    Charged Multivesicular Body Protein 4B (CHMP4B Human)
    Use
    Gateway cloning, flp-frt recombination, tet-induc…
    Tags
    Localization and Affinity Purification (LAP) tag
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Dec. 12, 2017
    Availability
    Academic Institutions and Nonprofits only
    101850-plasmid-map-sequence-id-200248

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  9. pAAV-nEF-Con/Fon DREADD Gi-mCherry

    Plasmid
    #177672
    Purpose
    Expresses DREADD hM4D (Gi) in Cre and Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    nEF
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177672-plasmid-map-sequence-id-348533

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  10. H2B-sfGFP-T2A-iCre

    Plasmid
    #178546
    Purpose
    pCMV-Histone H2B-sfGFP-T2A-iCre-bpA-FRT-Neo*-FRT
    Depositor
    Mario Capecchi
    Insert
    Histone H2B-sfGFP-T2A-iCre
    Use
    Cre/Lox and Mouse Targeting; Flp/frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 22, 2022
    Availability
    Academic Institutions and Nonprofits only
    178546-plasmid-map-sequence-id-360157

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  11. pAAV-nEF-Con/Foff DREADD Gi-mCherry

    Plasmid
    #177673
    Purpose
    Expresses DREADD hM4D (Gi) only in Cre expressing cells and not in Flp expressing cells
    Depositor
    Karl Deisseroth
    Has Service
    AAV8
    Insert
    DREADD Gi-mCherry
    Use
    AAV, Cre/Lox, and Mouse Targeting ; Intrsect
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    nEF
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177673-plasmid-map-sequence-id-348532

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  12. pSAH66 - [BAGp | gfp | tbb-2 UTR]

    Plasmid
    #200340
    Purpose
    gfp BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [BAGp | gfp | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00001536, flp-17
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    200340-plasmid-map-sequence-id-418120

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  13. pSAH65 - [BAGp | wrmScarlet | tbb-2 UTR]

    Plasmid
    #200339
    Purpose
    mScarlet BioPart for BAG neurons. (1) Mark neurons or (2) cell-specific expression of transgenes (N or C-terminal fusions, or untagged). Compatible with single-copy insertion (MosTI) or arrays.
    Depositor
    Christian Froekjaer-Jensen
    Insert
    [BAGp | wrmScarlet | tbb-2 UTR]
    Use
    Tags
    Expression
    Worm
    Mutation
    Promoter
    WBGene00001536, flp-17
    Available since
    Oct. 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    200339-plasmid-map-sequence-id-409767

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  14. tRECs/3xUAS-FP attB

    Plasmid
    #220330
    Purpose
    encodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)
    Depositor
    Erdem Bangi
    Inserts
    B3RT-STOP-B3RT-TagBFP-3xHA
    KDRT-STOP-KDRT-GFP-HSV
    FRTm71-STOP-FRTm71-mCherry
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Available since
    Aug. 7, 2024
    Availability
    Academic Institutions and Nonprofits only
    220330-plasmid-map-sequence-id-441802

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  15. FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector

    Plasmid
    #182346
    Purpose
    PacI-flanked CAG-LSL expression cassette with a FNF for positive selection
    Depositor
    Mario Capecchi
    Insert
    tdTomato
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 31, 2022
    Availability
    Academic Institutions and Nonprofits only
    182346-plasmid-map-sequence-id-358221

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  16. mT2A-sfGFP-linker-iCre-ERT2-FNF

    Plasmid
    #182341
    Purpose
    Recombineering cassette plasmid for T2A-sfGFP-linker-iCre-ERT2-FNF
    Depositor
    Mario Capecchi
    Insert
    T2A-sfGFP-linker-iCre-ERT2-FNF
    Use
    Mouse Targeting; Flp / frt
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    182341-plasmid-map-sequence-id-359742

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  17. pAAV-hSyn-frt-DIO-hM3D(Gq)-mCherry

    Plasmid
    #217397
    Purpose
    Expresses the hM3D(Gq) excitatory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoter
    Depositor
    Oscar Marin
    Insert
    hM3D(Gq)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin 1
    Available since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217397-plasmid-map-sequence-id-433248

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  18. pAAV-hSyn-frt-DIO-hM4D(Gi)-mCherry

    Plasmid
    #217398
    Purpose
    Expresses the hM4D(Gi) inhibitory DREADD fused to mCherry, in a Cre-ON and FLP-OFF dependent manner, under the control of hSyn promoter
    Depositor
    Oscar Marin
    Insert
    hM4D(Gi)-mCherry
    Use
    AAV
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    human Synapsin 1
    Available since
    May 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    217398-plasmid-map-sequence-id-433249

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Showing: 721 - 740 of 1327 results