Addgene

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pBiT3.1-N_SKIC8:1-305

Plasmid

#211038

Purpose

Mammalian expression of full-length human SKIC8. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

SKIC8:M1-I305 (WDR61 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_RBBP4:1-425

Plasmid

#211037

Purpose

Mammalian expression of full-length human RBBP4. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

RBBP4:M1-S425 (RBBP4 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-N_CCT5:1-541

Plasmid

#210978

Purpose

Mammalian expression of full-length human CCT5. N-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CCT5:M1-E541 (CCT5 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pNLF1-C_CCT5:1-541

Plasmid

#210979

Purpose

Mammalian expression of full-length human CCT5. C-terminal NanoLuc luciferase tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CCT5:M1-E541 (CCT5 Human)

Use

Tags

NanoLuc luciferase

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti NL

Plasmid

#113450

Purpose

Lentiviral NanoLuc control expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

NanoLuc

Use

Lentiviral and Luciferase

Tags

cmyc

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti myc-NL-GW

Plasmid

#113454

Purpose

Lentiviral NanoLuc Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral and Luciferase

Tags

cmyc-NanoLuc

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 myc-NL-GW

Plasmid

#113446

Purpose

NanoLuc Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

cmyc-NanoLuc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-NL-myc

Plasmid

#113447

Purpose

NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Luciferase; Gateway shuttle vector

Tags

NanoLuc-cmyc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 GW-mCit-PA

Plasmid

#113449

Purpose

ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

mCitrine-ProteinA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-GW

Plasmid

#113448

Purpose

ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Gateway shuttle vector

Tags

ProteinA-mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 31, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti GW-mCit-PA

Plasmid

#113457

Purpose

Lentiviral ProteinA-mCitrine Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral

Tags

mCitrine-ProteinA

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti GW-NL-myc

Plasmid

#113455

Purpose

Lentiviral NanoLuc Gateway shuttle vector for C-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral and Luciferase

Tags

NanoLuc-cmyc

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti PA-mCit-NL

Plasmid

#113452

Purpose

Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine-NanoLuc

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

hUBbC

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-NL

Plasmid

#113445

Purpose

ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-NanoLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 PA-mCit-NL

Plasmid

#113444

Purpose

ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine-NanoLuc

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti PA-mCit-GW

Plasmid

#113456

Purpose

Lentiviral ProteinA-mCitrine Gateway shuttle vector for N-terminal fusions

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Type

Empty backbone

Use

Lentiviral

Tags

ProteinA-mCitrine

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PA-NL

Plasmid

#113453

Purpose

Lentiviral ProteinA-NanoLuc hybrid fusion control expression vector (for BRET and LuC normalization)

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-NanoLuc

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

hUbC

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti PA-mCit

Plasmid

#113451

Purpose

Lentiviral ProteinA-mCitrine expression vector

Depositor

Erich Wanker

Article

Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

Insert

ProteinA-mCitrine

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

hUBbC

Available since

Aug. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pMRX-IRES-puro-3xFLAG

Plasmid

#204545

Purpose

Expresses 3xFLAG tag in mammalian cells

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

3xFlag

Use

Retroviral

Tags

Expression

Mammalian

Mutation

None

Promoter

Available since

Aug. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pT22gCfpUbiG4FER4P2A(#249)

Plasmid

#184070

Purpose

cyclofen-inducible GAL4 activation in zebrafish permanent transgenic

Depositor

Sophie Vriz

Article

Feng et al Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.

Insert

Gal4bdFF-ERT2-P2A

Use

Zebrafish transgenesis (blue eyes)

Tags

Expression

Mutation

ERT2: G400V, M543A, L544A, deleted 1-281;

Promoter

Zf-Ubi

Available since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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