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Showing: 1441 - 1480 of 23151 results

  9. pJV53-Cas12a

    Plasmid
    #158706
    Purpose
    CRISPR system used for genome editing in Mycobacterium smegmatis, expresses codon-optimized FnCpf1 and the recombination proteins gp60 and gp61
    Depositor
    Yi-Cheng Sun
    Article
    Yan et al Appl Environ Microbiol. 2017 Aug 17;83(1
    Insert
    FnCpf1
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    TetO
    Available since
    Jan. 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    158706-plasmid-map-sequence-id-320865

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  17. pJAT57

    Plasmid
    #204303
    Purpose
    Expresses Cas9 under UAS control. HDR integrant markers with DsRed expressed in flight muscles.
    Depositor
    David Stern
    Article
    Stern et al bioRxiv 2023.06.17.545412
    Insert
    MHC-DsRed
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    MHC
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    204303-plasmid-map-sequence-id-405488

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  18. pCBC-MT3T4

    Plasmid
    #50595
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Article
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T3, gRNA scaffold, U6-26t plus, OsU3p, T4
    Use
    CRISPR; Pcr template
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    50595-plasmid-map-sequence-id-75675

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  21. pADH99

    Plasmid
    #90979
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH100-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    US_HIS1
    FRT
    pENO1
    C. albicans Cas9
    pMAL2
    FLP
    NAT 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90979-plasmid-map-sequence-id-180505

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  22. pCB-CBEv1

    Plasmid
    #221138
    Purpose
    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.
    Depositor
    Craig Roy
    Article
    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1
    Insert
    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Bacterial
    Mutation
    Promoter
    lacIq-Ptac
    Available since
    Nov. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    221138-plasmid-map-sequence-id-445118

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  27. pADH100

    Plasmid
    #90980
    Purpose
    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb HIS1-FLP CRISPR system. Use with pADH99 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, FRT, DS_HIS1
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90980-plasmid-map-sequence-id-180507

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  33. pADH118

    Plasmid
    #90983
    Purpose
    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH137 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. albicans LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90983-plasmid-map-sequence-id-180511

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  34. pADH137

    Plasmid
    #90986
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.alb LEUpOUT CRISPR system. Use with pADH118-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    C. albicans LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    90986-plasmid-map-sequence-id-180519

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  35. pADH143

    Plasmid
    #90989
    Purpose
    NAT-marked "empty" gRNA expression construct; part 2 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH140 CAS9 expression construct.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Insert
    NAT 2 of 2, pSNR52, empty gRNA, gRNA conserved, C. mal LEU2 2 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90989-plasmid-map-sequence-id-180522

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  36. pADH140

    Plasmid
    #90988
    Purpose
    NAT-marked CAS9 expression construct; part 1 of 2 of C.mal LEUpOUT CRISPR system. Use with pADH143-series gRNA expression constructs.
    Depositor
    Aaron Hernday
    Article
    Nguyen et al mSphere. 2017 Apr 26;2(2):e00149-17. doi
    Inserts
    C. maltosa LEU2 1 of 2
    pENO1
    Cas9
    NAT 1 of 2
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 5, 2017
    Availability
    Academic Institutions and Nonprofits only
    90988-plasmid-map-sequence-id-180521

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  37. pAEF5

    Plasmid
    #136305
    Purpose
    Plasmid encoding the Cas9 gene and a gRNA expression cassette allowing to clone a single or multiple gRNAs for DSBs induction in yeast. Constructed by Aubin Fleiss
    Depositor
    Gilles Fischer
    Article
    Agier et al Methods Mol Biol. 2021;2196:181-198. doi
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    136305-plasmid-map-sequence-id-271550

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Showing: 1441 - 1480 of 23151 results