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Plasmid#222144PurposeAdenine base editor with modified TadA variant for restricted A-to-G base editing in zebrafishDepositorInsertModified TadA-nSpCas9
UseCRISPR; Zebrafish expressionTagsBipartite NLSExpressionMutationPromoterT3Available sinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
MSP1673
Plasmid#65769PurposeBacterial expression plasmid for S. thermophilus1 Cas9 & sgRNA (need to clone in spacer into BspMI sites): T7-humanSt1Cas9-NLS-T7-BspMIcassette-St1-sgRNADepositorInsertmammalian codon-optimized Streptococcus thermophilus1 Cas9-NLS, and St1Cas9 gRNA
UseCRISPRTagsNLSExpressionBacterialMutationPromoterT7 (x2)Available sinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
Cas9-2A-Cre
Plasmid#68468Purposeco-expressing Cas9 and Cre recombinaseDepositorInsertCas9 and Cre recombinase
UseCRISPRTagsP2A-Cre-MycExpressionMammalianMutationPromoterCAGAvailable sinceNov. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
wt dynamin 1 pEGFP
Plasmid#34680DepositorInsertdynamin 1ba (DNM1L Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2012AvailabilityAcademic Institutions and Nonprofits only -
dCBE4-gam
Plasmid#124449PurposeExpresses dead Cas9 (dCas9)-CBE4-gamDepositorInsertdCBE4-gam
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationD10A, H840APromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-S in pAR9 (rep/cap)
Plasmid#174539Purposeencodes AAV-S capsid for efficient gene delivery to the mammalian cochleaDepositorInsertVP1 of AAV9 containing the AAV-S 7-mer peptide insert
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1-alpha-IIb
Plasmid#27288DepositorInsertintegrin alpha IIb (ITGA2B Human)
UseTagsHis and V5ExpressionMammalianMutationn/aPromoterAvailable sinceJan. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
K44A dynamin 1 pEGFP
Plasmid#34681DepositorInsertdynamin 1ba (DNM1L Human)
UseTagsEGFPExpressionMammalianMutationK44APromoterAvailable sinceJan. 31, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmPAN3-W707AG711E-dsRNAres_Y
Plasmid#148087PurposeInsect Expression of DmPAN3-W707AG711E-dsRNAresDepositorInsertDmPAN3-W707AG711E-dsRNAres (CG11486 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDL-hu FceRI alpha
Plasmid#8365DepositorInserthuman Fc epsilon RI alpha cDNA (FCER1A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLKO mouse shRNA 1 raptor
Plasmid#21339DepositorInsertraptor shRNA 1 (Rptor Mouse)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceJuly 7, 2009AvailabilityAcademic Institutions and Nonprofits only -
pOPS0263
Plasmid#133228PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), gusA (pOGG083) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPS0262
Plasmid#133227PurposeReporter plasmid for R. leguminosarum suitable for experiments in environments where there is no antibiotic selection present.DepositorInsertconstructed with PsNifH (pOGG043), celB (pOGG050) and T-pharma (pOGG003)
UseTagsExpressionBacterialMutationDomesticated for Golden Gate cloningPromoterAvailable sinceApril 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEX5X.1 PINK1 WT
Plasmid#13321DepositorInsertPINK1 kinase domain (PINK1 Human)
UseTagsGSTExpressionBacterialMutationAmino acids 112-496PromoterAvailable sinceNov. 9, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCX-NTOM-SpiCee
Plasmid#140902Purposemammalian expression of NTOM-SpiCee, a mitochondria-targeted variant of the calcium buffer SpiCee that prevents downstream signalingDepositorInsertNTOM-SpiCee
UseTagsmRFPExpressionMammalianMutationPromoterCAGAvailable sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfEpoa
Plasmid#65611PurposeExpress soluble His-tagged zebrafish erythropoietin in E. coliDepositorInserterythropoietin (epoa Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7Available sinceSept. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVRb20_992
Plasmid#49714DepositorInsertsfgfp
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPecf20_992Available sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHS1184
Plasmid#205965PurposeNZ_CP025815 His14-TwinStrep-bdSUMO tagged DinG HNH protein (E. coli codon optimized) in pET45b(+)DepositorInsertDinG HNH
UseTags14xHis-TwinStrep-bdSUMOExpressionBacterialMutationPromoterT7Available sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHis-zfCSF3b
Plasmid#65613Purposeexpress soluble His-tagged zebrafish CSF3b/Gcsfb in E. coliDepositorInsertCsf3b (csf3b Zebrafish)
UseTags(His)6ExpressionBacterialMutationPromoterT7Available sinceAug. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLenti6-DEST PINK1-V5 G309D
Plasmid#13318DepositorInsertPINK1 (PINK1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationG309DPromoterAvailable sinceOct. 16, 2006AvailabilityAcademic Institutions and Nonprofits only