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pU6-sgRNA-CBh-eGFP

Plasmid

#206885

Purpose

cloning backbone for sgRNA using OptScf2

Depositor

Jacob Giehm Mikkelsen

Article

Haldrup et al Nucleic Acids Res. 2023 Sep 8:gkad676. d

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

U6

Available since

Oct. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EGFP-Rac1-T17N

Plasmid

#12982

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Rac1 DN (RAC1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

T17N Dominant negative

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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CAG-GFP-IRES-CRE

Plasmid

#48201

Purpose

retroviral vector encoding for GFP and Cre-recombinase

Depositor

Fred Gage

Article

Zhao et al J Neurosci. 2006 Jan 4. 26(1):3-11.

Inserts

eGFP
Cre

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CAG and linked by IRES

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLPCX cyto Grx1-roGFP2

Plasmid

#64975

Purpose

Mammalian expression of cytosolic Grx1-roGFP2 (retroviral vector)

Depositor

Tobias Dick

Article

Gutscher et al Nat Methods. 2008 Jun;5(6):553-9. doi: 1

Insert

Glutaredoxin-1 (GLRX Human)

Use

Retroviral

Tags

roGFP2

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_ManII-SBP-EGFP

Plasmid

#65252

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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pUC19 - T7 pro - IRES - EGFP

Plasmid

#138586

Purpose

Express EGFP with an upstream T7 promoter in pUC19 backbone

Depositor

Fei Chen

Article

Chen et al Nat Biotechnol. 2019 Dec 16. pii: 10.103

Insert

EGFP

Use

Tags

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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mouse E-cadherin GFP (423)

Plasmid

#67937

Purpose

Mammalian expression of E-cadherin-GFP

Depositor

Alpha Yap

Article

Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

Insert

E-cadherin (Cdh1 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pIG-601_NY-ESO-1_TCR_GFP

Plasmid

#207482

Purpose

This plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

GFP, NY-ESO-1_TCR

Use

CRISPR

Tags

Expression

Mutation

Potential silent point mutation in GFP sequence

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-eGFP-53BP1

Plasmid

#60813

Purpose

mammalian expression vector

Depositor

Daniel Durocher

Article

Fradet-Turcotte et al Nature. 2013 Jul 4;499(7456):50-4. doi:

Insert

53BP1 (TP53BP1 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 9, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-EGFP-Cdc42-Q61L

Plasmid

#12986

Depositor

Gary Bokoch

Article

Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

Insert

Cdc42 constitutively active (CDC42 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Q61L Constitutively active

Promoter

Available since

Oct. 27, 2006

Availability

Academic Institutions and Nonprofits only

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mini-donor - NKX2.5-nEGFP

Plasmid

#209287

Purpose

this mini-donor knockin vector can be used to knock nuclear EGFP into NKX2.5 locus

Depositor

Xiaojun Lian

Article

Haideri et al Stem Cell Reports, 2024

Insert

EGFP-nls

Use

Knockin donor vector

Tags

Expression

Mutation

Promoter

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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FUGW-d2GFP-ZEB1 3'UTR

Plasmid

#79601

Purpose

FUGW lentiviral vector with constitutively expressed destabilized GFP (d2GFP) regulated by the human ZEB1 3'UTR. When used in combination with Ecad-RFP, comprises the Z-cad dual sensor.

Depositor

Jeffrey Rosen

Article

Toneff et al BMC Biol. 2016 Jun 17;14(1):47.

Insert

d2GFP-Zeb1 3'UTR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pFUGW-Pol2-ffLuc2-eGFP

Plasmid

#71394

Purpose

Lentiviral vector of luciferase-eGFP fusion gene driven by Pol2 promoter

Depositor

Glenn Merlino

Article

Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

Insert

Pol2-ffLuc2-eGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Pol2 (mouse RNA polymerase II gene promtoer)

Available since

Dec. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pKP112 (pRS406-GFP-ADH1t)

Plasmid

#106472

Purpose

Vector for creating C-terminal GFP fusion proteins in yeast.

Depositor

Daniel Klionsky

Article

Parzych et al Mol Biol Cell. 2018 May 1;29(9):1089-109

Type

Empty backbone

Use

Tags

GFP

Expression

Bacterial and Yeast

Mutation

Promoter

None

Available since

June 5, 2018

Availability

Academic Institutions and Nonprofits only

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p415 TEF roGFP2‐Tsa2ΔCR

Plasmid

#83238

Purpose

The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCr

Use

Tags

roGFP2 fusion with Tsa2dCr

Expression

Yeast

Mutation

Promoter

TEF

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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cytoplasmic EKAR (EGFP-mRFP)

Plasmid

#18680

Depositor

Karel Svoboda

Article

Harvey et al PNAS 2008 Dec 9;105(49):19264-9

Insert

ERK Activity Reporter

Use

Tags

EGFP and mRFP

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-GFP-wt-lamin A

Plasmid

#17662

Depositor

Tom Misteli

Article

Scaffidi et al Nat Cell Biol. 2008 Mar 2. ():.

Insert

GFP-lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pRRL SFFV d20GFP.T2A.mTagBFP Donor

Plasmid

#31485

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d20BFP T2A BFP

Use

Lentiviral

Tags

Expression

Mutation

GFP has 20 amino acids deleted from the C terminu…

Promoter

Available since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pNL4-3 envFS eGFP gag2xFS

Plasmid

#101341

Purpose

1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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Str-Golgin84_VSVG-SBP-EGFP

Plasmid

#65305

Purpose

synchronize trafficking of Golgin-84 from the Golgi apparatus (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Golgin-84 (GOLGA5 Human)

Use

Tags

EGFP and Streptavidin Binding Protein (SBP)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2015

Availability

Academic Institutions and Nonprofits only

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