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Plasmid#176138PurposeHeterodimerization with FRB (rapamycin), Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ141D-peg
Plasmid#141081PurposeGateway compatible prime editing gRNA empty backbone vectorDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJune 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSW178 - pXPP
Plasmid#115987Purposeentry vector for GreenGate cloning method, promoterDepositorInsertpXPP
UseUnspecifiedTagsExpressionMutationPromoterAvailable sinceDec. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPSP6+t
Plasmid#48149PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMIG-Flag-RIPK1-wt
Plasmid#102677Purposeretroviral expression plasmid for RIPK1-wtDepositorInsertRIPK1 (RIPK1 Human)
UseRetroviralTagsFLAG and IRES-GFPExpressionMammalianMutationPromoterunknownAvailable sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
hBACCS2-IRES-mCherry
Plasmid#72892PurposeMammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch, and mCherryDepositorInserthBACCS2-IRES-mCherry
UseTagsIRES-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-DIO-hM4D(Gi)-mCherry
Plasmid#190240PurposeExpresses iDREADD fused to mCherry flanked by DIODepositorInserthM4D(Gi)-mCherry
UseAAVTagsmCherryExpressionMutationPromotermouse CamKII alphaAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKPY197
Plasmid#62599PurposeEncodes Thr412Gly-CePheRS Alpha SubunitDepositorInsertThr412Gly-CePheRS Alpha Subunit
UseTagsHISExpressionWormMutationThr412GlyPromoterAvailable sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-KRasCT
Plasmid#175835PurposeExpress mCherry with plasma membrane-targeting sequence in mammalian cellsDepositorInsertmCherry with plasma membrane-targeting sequence
UseTagsC-terminal hypervariable region of K-Ras 4BExpressionMammalianMutationPromoterAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree_KA2_ZIKV NS4B
Plasmid#145367PurposeCell-free/mammalian expression vector for Zika virus NS4Bprotein with N-term mCherryDepositorInsertZIKV NS4B
UseTags8xHis and mCherryExpressionBacterialMutationPromoterT7- CMV/CAGAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSLC-242
Plasmid#73189PurposeTemplate plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertchloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
UseTagsExpressionBacterialMutationPromoterchloramphenicol promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
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pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRSETb-rsGreen1
Plasmid#78180PurposeExpresses rsGreen1 in E. coli strains.DepositorInsertrsGreen1
UseTags6xHis-tag; Xpress tagExpressionBacterialMutationPromoterT7Available sinceMay 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-STAT3-linker-TagGFP2
Plasmid#121427PurposeWT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.DepositorInsertSTAT3-linker-TagGFP2
UseTagsTagGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSB161 - pL2_pSB90_2x35S::GUS::tMAS
Plasmid#123197Purposebinary plant vector for transient expression of GUS (with introns)DepositorInsert2x35S::GUS::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
hsa-miR-100 plix4.03
Plasmid#121128Purposeinducible expression in cellsDepositorInserthsa-miR-100 (MIR100 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mTagBFP2-WPRE
Plasmid#193332PurposeExpresses mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
UseTagsExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable sinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28-sfGFP[D134TAG+N150TAA]
Plasmid#164582Purposesuperfolder GFP with D134TAG and N150TAA mutationsDepositorInsertsfGFP-134TAG+150TAA
UseTags6xHisExpressionBacterialMutationD134TAG + N150TAA MutationsPromoterT7Available sinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
Lyn-FRB-GFP11
Plasmid#181863PurposePlasma membrane-localized GFP11; for plasma membrane targeting of FluoSTEPs.DepositorInsertLyn-FRB-GFP11
UseTagsGFP11 and N-terminal targeting sequence from Lyn …ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only