Addgene

We narrowed to 7,946 results for: sgRNA

Showing: 7341 - 7360 of 7946 results

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NFATc2-CRISPR-Nick 2/2 (PX461-EF1a-pSpCas9n(BB)-2A-GFP)

Plasmid

#75235

Purpose

CRISPR/Cas9 NICKASE plasmid against human NFATc2 (2/2)

Depositor

Ria Baumgrass

Article

Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

Insert

sgRNA against NFATc2 (NFATC2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-U6-sgLacZ-U6-sgGFP-hSyn1-mCherry

Plasmid

#208834

Purpose

Control plasmid expresses mCherry under the hSyn1 promoter with sgRNAs against LacZ and GFP

Depositor

Ben Emery

Article

Duncan et al Nat Commun. 2024 Oct 23;15(1):9148. doi:

Insert

mCherry

Use

AAV and CRISPR

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

hSyn1

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

Plasmid

#163701

Purpose

Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Inserts

SOX2-3xHA-P2A-tagBFP (SOX2 Human)
rtTA
T2-sgRNA

Use

AAV and CRISPR

Tags

3x-HA and P2A-tagBFP

Expression

Mammalian

Mutation

Promoter

CAG and TRE3G

Available since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD T456A S1406A S1859A

Plasmid

#169826

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K, PKA, and Erk1/2 phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1406A S1859A; TCCC -> AGTC silent mutat…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD R2187A

Plasmid

#169827

Purpose

Expresses C-terminal flag-tagged CAD with mutation at reported trimerization interface of the ATCase domain of CAD

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

R2187A, T456A S1406A S1859A; TCCC -> AGTC sile…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD M1601E

Plasmid

#169828

Purpose

Expresses C-terminal flag-tagged CAD with mutation at reported dimerization interface of the DHOase domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

M1601E; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD H1734A H1741A

Plasmid

#169830

Purpose

Expresses C-terminal flag-tagged CAD with mutations at reported dimerization interface of the DHOase domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

H1734A H1741A; TCCC -> AGTC silent mutations a…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD delta Loop1

Plasmid

#169831

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids F1366 - P1376 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletion of amino acids F1366 - P1376; TCCC ->…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD delta Loop2

Plasmid

#169832

Purpose

Expresses C-terminal flag-tagged CAD with deletion of amino acids R1398 - S1407 in allosteric domain

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

Deletion of amino acids R1398 - S1407; TCCC ->…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD

Plasmid

#169818

Purpose

Expresses C-terminal flag-tagged CAD in mammalian cells

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

TCCC -> AGTC silent mutations at nt527-530 eli…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1406A

Plasmid

#169820

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1406A; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-BLAST flag-CAD S1859A

Plasmid

#169821

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1859A; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD S1859E

Plasmid

#169822

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

S1859E; TCCC -> AGTC silent mutations at nt527…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD T456A S1406A

Plasmid

#169823

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and PKA phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1406A; TCCC -> AGTC silent mutations at…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMXS-IRES-BLAST flag-CAD T456A S1859A

Plasmid

#169824

Purpose

Expresses C-terminal flag-tagged CAD with mutation of reported Erk1/2 and S6K phosphorylation site

Depositor

Richard Possemato

Article

Shin et al Nat Metab. 2023 Feb 6. doi: 10.1038/s422

Insert

CAD (CAD Human)

Use

Retroviral

Tags

Flag tag

Expression

Mammalian

Mutation

T456A S1859A; TCCC -> AGTC silent mutations at…

Promoter

Available since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZNF90_5-5)-PGKpuroBFP-W

Plasmid

#212003

Purpose

Express gRNA against ZNF90 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZNF90 (ZNF90 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(VENTX_5-5)-PGKpuroBFP-W

Plasmid

#211993

Purpose

Express gRNA against VENTX with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting VENTX (VENTX Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZIC2_5-4)-PGKpuroBFP-W

Plasmid

#211994

Purpose

Express gRNA against ZIC2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZIC2 (ZIC2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZIC2_5-5)-PGKpuroBFP-W

Plasmid

#211995

Purpose

Express gRNA against ZIC2 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZIC2 (ZIC2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(ZIC3_5-3)-PGKpuroBFP-W

Plasmid

#211996

Purpose

Express gRNA against ZIC3 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting ZIC3 (ZIC3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 7341 - 7360 of 7946 results

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