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PX551

Plasmid

#60957

Purpose

pAAV-pMecp2-SpCas9-spA (AAV-SpCas9). AAV plasmid expressing Cas9 in neurons under control of truncated mecp2 promoter.

Depositor

Feng Zhang

Article

Swiech et al Nat Biotechnol. 2014 Oct 19. doi: 10.103

Insert

SpCas9

Use

AAV and CRISPR

Tags

HA

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Nov. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Delta(Pro-MP)ADAM17-HA

Plasmid

#65221

Purpose

Expression of HA tagged ADAM17 lacking prodomain and metalloprotease domain in mammalian cells

Depositor

Axel Ullrich

Article

Gschwind et al EMBO J. 2003 May 15;22(10):2411-21.

Insert

Delta(Pro-MP)ADAM17 (ADAM17 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

deleted AA 18-473

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only
14x PP7 with loxP-kanMX-loxP

Plasmid

#189939

Purpose

Integration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)

Depositor

Tineke Lenstra

Article

Donovan et al EMBO J. 2019 Jun 17;38(12). pii: embj.20

Insert

14x PP7 with loxP-kanMX-loxP

Use

Tags

kanMX

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pmTurquoise2-C1

Plasmid

#60560

Purpose

Expresses the cyan fluorescent protein variant mTurquoise2, can be used to create fusion proteins

Depositor

Dorus Gadella

Article

Goedhart et al Nat Commun. 2012 Mar 20;3:751. doi: 10.1

Insert

mTurquoise2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 31, 2014

Availability

Academic Institutions and Nonprofits only

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pLPC-NMYC TRF2

Plasmid

#16066

Depositor

Titia de Lange

Article

Smogorzewska et al EMBO J. 2002 Aug 15. 21(16):4338-48.

Insert

hTRF2 (TERF2 Human)

Use

Retroviral

Tags

Myc

Expression

Mutation

aa2-500, inserted alanin at aa434

Promoter

Available since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 2

Plasmid

#133771

Purpose

express 6Xhis- SUMO 2 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 2 (SUMO2 Human)

Use

Tags

6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pHG6

Plasmid

#188254

Purpose

Integrating plasmid contains the BT1 int/attP locus and the erythromycin-resistance gene for selection

Depositor

Maggie Smith, Hong Gao

Article

Gao et al Access Microbiol. 2021 Dec 8;3(12):00029

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

act1p/actII-orf4

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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DR274

Plasmid

#42250

Purpose

guide RNA (gRNA) expression vector used to create a gRNA to a specific sequence; uses T7 promoter for in vitro transcription

Depositor

Keith Joung

Article

Hwang et al Nat Biotechnol. 2013 Jan 29. doi: 10.103

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR; Zebrafish expression

Tags

Expression

Worm

Mutation

Promoter

T7

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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GFP Snail 6SA

Plasmid

#16228

Depositor

Mien-Chie Hung

Article

Zhou et al Nat Cell Biol. 2004 Oct . 6(10):931-40.

Insert

Snail (SNAI1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

6SA: S97A, S101A, S108A, S112A, S116A, S120A

Promoter

Available since

Nov. 30, 2007

Availability

Academic Institutions and Nonprofits only

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pXL001-TQM IL13 CAR

Plasmid

#154054

Purpose

Lentiviral expression of TQM IL13 CAR for CAR-T therapy

Depositor

Xiaojun Lian

Article

Kim et al Bioact Mater. 2020 May 7;5(3):624-635. d

Insert

TQM IL13 CAR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1a

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3_cAMPFIRE-M

Plasmid

#182280

Purpose

Mammalian expression of the cAMP sensor cAMPFIRE-M under a CMV promotor.

Depositor

Haining Zhong

Article

Massengill et al Nat Methods. 2022 Nov;19(11):1461-1471.

Insert

cAMPFIRE-M (cAMP sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2022

Availability

Academic Institutions and Nonprofits only

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mH2A1.2-CT-GFP

Plasmid

#45169

Depositor

Brian Chadwick, Hunt Willard

Article

Chadwick et al Hum Mol Genet. 2001 May 1;10(10):1101-13

Insert

H2Afy (MACROH2A1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

July 2, 2013

Availability

Academic Institutions and Nonprofits only

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pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

Plasmid

#113865

Purpose

Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

Depositor

Manfred Ogris

Article

Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

Inserts

CMV-eGFP
TOP-iRFP
PTCH1-mTurquoise2
CBF-tdTomato

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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p181 pK7-HDAC1 (GFP)

Plasmid

#11054

Depositor

Ramesh Shivdasani

Article

Tou et al Mol Cell Biol. 2004 Apr . 24(8):3132-9.

Insert

HDAC1 (Hdac1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

April 19, 2006

Availability

Academic Institutions and Nonprofits only

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Anti-Cav3.2 Ca2+ channel [N55/10R]

Plasmid

#177562

Purpose

Mammalian Expression Plasmid of anti-Cav3.2 Ca2+ channel (Human). Derived from hybridoma N55/10.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Cav3.2 Ca2+ channel (Homo sapiens) recombinant mouse monoclonal antibody (CACNA1H Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-PPP2R2B

Plasmid

#16181

Depositor

Huda Zoghbi

Article

Lim et al Cell. 2006 May 19. 125(4):801-14.

Insert

PP2A regulatory subunit B, beta isoform (PPP2R2B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pRK5-HA GST RagA 66L

Plasmid

#19300

Depositor

David Sabatini

Article

Sancak et al Science. 2008 Jun 13. 320(5882):1496-501

Insert

Ras-related GTP binding A (RRAGA Human)

Use

Tags

GST and HA

Expression

Mammalian

Mutation

Q66L

Promoter

Available since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only

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EGFR-mEGFP

Plasmid

#203774

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Gupta et al J Lipid Res. 2022 Jun;63(6):100220. doi:

Insert

EGFR (EGFR Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

July 11, 2023

Availability

Academic Institutions and Nonprofits only

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Human SERCA2b (pcDNA3.1+)

Plasmid

#75188

Purpose

Mammalian expression of ATP2A2

Depositor

David MacLennan, Jonathan Lytton

Article

Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

Insert

ATP2A2 (ATP2A2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pRSET.HaloTag.v7.AviTag

Plasmid

#195486

Purpose

Bacterial expression plasmid with Halotag v7 insert plus a C-terminus appended AviTag for enzymatic biotinylation.

Depositor

HHMI-JRC Tool Translation Team, Tim Brown

Insert

HaloTag.v7.AviTag

Use

Tags

6xHIS and AviTag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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