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Plasmid#107524PurposeNegative regulator of cell cycle progression by G1/S transition inhibitionDepositorInsertCdkn1b.b
UseTagsExpressionMutationPromoterTVC-specific FoxF enhancer (FoxFTVC+bpFOG)Available sinceApril 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWDY1226
Plasmid#138593PurposeExpresses NatL2-R156A in E.coliDepositorInsertphenylacetate-coenzyme A ligase
UseTags6-HisExpressionBacterialMutationchanged Arginine 156 to AlaninePromoterT7Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWDY1222
Plasmid#138592PurposeExpresses NatL2-K418A in E.coliDepositorInsertphenylacetate-coenzyme A ligase
UseTags6-HisExpressionBacterialMutationchanged Lysine 418 to AlaninePromoterT7Available sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS039
Plasmid#134233PurposeFor in vitro transcription of Miniata CENP-ADepositorInsertMiniata CENP-A n-terminus (nt 1-204, aa 1-68)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZS077
Plasmid#134235PurposeBacterial expression of CENP-CDepositorInsertGST-CENP-C
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5343_UC57
Plasmid#126645Purposeenvironmental plasmid p5343 cloned into pUC57DepositorInsertEnvironmental plasmid p5343
UseSynthetic BiologyTagsExpressionMutationPromoterNAAvailable sinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pANTO3
Plasmid#131107PurposeTo generate a replicative plasmid carrying mycobacteriophage L5 integraseDepositorInsertmycobacteriophage L5 integrase
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSADuet148
Plasmid#121910PurposeCharge-introduced NpuDnaBΔ283ΔC39 inteinDepositorInsertCI-NpuDnaBΔ290ΔC39 intein
UseTagsHis6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet259
Plasmid#121911PurposeNpuDnaBΔ283ΔC39 inteinDepositorInsertNpuDnaBΔ283ΔC39 intein
UseTagsHis6-GB1ExpressionBacterialMutationDeletion of 283-residue endonuclease domainPromoterT7Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBBAD113
Plasmid#121912PurposeCharge-Introduced NpuDnaBC39 inteinDepositorInsertCI-NpuDnaBC39 intein
UseTagsGB1-His6ExpressionBacterialMutationS110K, I111KPromoterParaAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBDuet116
Plasmid#121915PurposeNpuDnaBΔ290ΔC39 inteinDepositorInsertOth-NpuDnaBΔ290ΔC39 intein
UseTagsHis6-GB1ExpressionBacterialMutationDeletion of 290-residue endonuclease domain, I53K…PromoterT7Available sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHBBAD168
Plasmid#121916PurposeOth-NpuDnaBC39 inteinDepositorInsertOth-NpuDnaBC39 intein
UseTagsGB1-His6ExpressionBacterialMutationI111K, E116KPromoterParaAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0517
Plasmid#133492PurposeExpression of nucleo-cytoplasmic GFP in P-cellsDepositorInsertpHis5(StuI)-p(map3)-GFP-terminator(nmt)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0772
Plasmid#133516PurposeExpression of sfGFP tagged beta-tubulin to monitor microtubulesDepositorInsertpAde6(PmeI)-p(atb2)-sfGFP-atb2-terminator(atb2)-hphMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0607
Plasmid#133504PurposeExpression of mCherry targeted to the plasma membrane with an amphipathic helix of RitCDepositorInsertpAde6(PmeI)-p(act1)-mCherry-RitC-terminator(ScADH1)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAV0763
Plasmid#133497PurposeExpression of nucleo-cytoplasmic mTagBFP2 in M-cellsDepositorInsertpAde6(PmeI)-p(mam1*)-mTagBFP2-terminator(ScADH1)-kanMX
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabA2
Plasmid#133001PurposepFE carrying a dabA2 gene (Uniprot: D0KWS7)DepositorInsertdabA2
UseTagsExpressionBacterialMutationPromotertetRAvailable sinceNov. 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFE-dabB2
Plasmid#133000PurposepFE carrying a dabB2 gene (Uniprot: D0KWS8)DepositorInsertdabA2
UseTagsExpressionBacterialMutationPromotertetRAvailable sinceNov. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLPhygSaCas9
Plasmid#123262PurposeExpresses Staphylococcus aureus Cas9 (SaCas9) in LeishmaniaDepositorInsertStaphylococcus aureus Cas9 (NEWENTRY )
UseCRISPR; LeishmaniaTagsExpressionMutationPromoterAvailable sinceAug. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFGL1259R
Plasmid#118995PurposemCherry (without start and stop codons) in pGFL1010DepositorInsertmCherry (without start and stop codons)
UseFungal expressionTagsExpressionMutationPromoterpromoter lessAvailable sinceAug. 19, 2019AvailabilityAcademic Institutions and Nonprofits only