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pcDNA5-FRT-TO-Nsp5-HF

Plasmid

#157687

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp5 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp5-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pGEX-GP-2-ATRX ADD

Plasmid

#59698

Purpose

Contains histone modification interacting domain (ATRX ADD)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

ATRX ADD (ATRX Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

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Beta2-AR-TYY

Plasmid

#32694

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2006 Jan 13;281(2):1261-73.

Insert

β2AR (ADRB2 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

T68F, Y132G Y219A

Promoter

CMV

Available since

Nov. 18, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-Nsp16-HF

Plasmid

#157725

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp16 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp16-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-FH-Nsp2

Plasmid

#157683

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp2 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp2 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 4, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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GFP-Epac1 CAAX

Plasmid

#118312

Purpose

Expression of human Epac1

Depositor

Philip Stork

Article

Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

Insert

Epac1 CAAX (RAPGEF3 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C-terminal 20aa of KiRas

Promoter

CMV

Available since

Nov. 5, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-MPRA-MluI-SpeI-EcoRI

Plasmid

#190196

Purpose

Empty vector for inserting MPRA libraries into AAV

Depositor

Hyejung Won

Article

McAfee et al Cell Genom. 2023 Sep 15;3(10):100404. do

Type

Empty backbone

Use

AAV; Massively parallel reporter assay (mpra)

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2024

Availability

Academic Institutions and Nonprofits only

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pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

Plasmid

#22023

Depositor

Klaus Hahn

Article

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Tags

Expression

Mammalian

Mutation

The LOV domain contains the mutation C450A.Rac1 s…

Promoter

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pU6-neo-TET

Plasmid

#51286

Purpose

expresses U6 RNA, using its own pol III enhancer sequence and tagged with the self splicing intron neo cassette

Depositor

Astrid Roy-Engel

Article

Kroutter et al PLoS Genet. 2009 Apr;5(4):e1000458. doi:

Insert

U6 (RNU6-1 Human)

Use

Tags

neoTET cassette with a tetrahymena self-splicing …

Expression

Mammalian

Mutation

Promoter

U6 upstream enhancer sequence

Available since

April 7, 2014

Availability

Academic Institutions and Nonprofits only

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pCS2-CRY2

Plasmid

#140572

Purpose

For use in light-induced protein inactivation through the interaction with CIB1

Depositor

Heidi Hehnly

Article

Rathbun et al Nat Commun. 2020 Mar 9;11(1):1269. doi:

Insert

CRY2 (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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Polymerase expression - pCMV-B103 (LM2907)

Plasmid

#208968

Purpose

Unfused B103 DNA polymerase, expressed from CMV or T7 promoters.

Depositor

Benjamin Kleinstiver

Article

Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

Insert

B103-BPNLS

Use

CRISPR; In vitro transcription; t7 promoter

Tags

BPNLS

Expression

Mammalian

Mutation

Promoter

CMV and T7

Available since

Nov. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-FH-Nsp13

Plasmid

#157713

Purpose

mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp13 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

FH-Nsp13 (ORF1ab SARS-CoV-2)

Use

Tags

Flag-His8

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 6, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pTag-RFP-C-h-Rab4a-c-Myc

Plasmid

#79800

Purpose

Express TagRFP labeled human Rab5a (GTPase located on recycling endosomal membranes).

Depositor

James Johnson

Article

Boothe et al Mol Metab. 2016 Feb 10;5(5):366-78. doi:

Insert

Rab4a (RAB4A Human)

Use

Tags

TagRFP and myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA5-FRT-TO-Nsp12-HF

Plasmid

#157711

Purpose

mammalian expression of C-terminally His8-Flag tagged SARS-CoV-2 Nsp12 under control of a tetracycline-inducible promoter

Depositor

David Tollervey

Article

Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

Insert

Nsp12-HF (ORF1ab SARS-CoV-2)

Use

Tags

His8-Flag

Expression

Mammalian

Mutation

codon optimized

Promoter

Available since

Aug. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCW57.1-pDUXChd:hDUX4ctd-ca

Plasmid

#198237

Purpose

Lentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

DUX4L pDUXChd:hDUX4ctd (codon altered) (DUX4 Human, S. scrofa (porcine))

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C3-PLK4-S305A-3xFLAG

Plasmid

#69839

Purpose

This plasmid encodes PLK4 isoform 1 carrying an S305A mutation with an N-terminal EGFP tag and C-terminal 3xFLAG tag

Depositor

Michel Bornens

Article

Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

Insert

PLK4 (PLK4 Human)

Use

Tags

3xFLAG tag and EGFP

Expression

Mammalian

Mutation

S305A

Promoter

CMV

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1APΔC20-WPRE

Plasmid

#127865

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APΔC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

deleted the c-terminal 20 amino acids

Promoter

hSyn

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR Del4

Plasmid

#32064

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Delete L747-P753, insert S

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
LN-entry+NLS

Plasmid

#128011

Purpose

Vector containing the lambdaN-entry expression cassette with SV40 NLS and a separate expression cassette driving mCherry via the traffic jam enhancer

Depositor

Julius Brennecke

Article

Batki et al Nat Struct Mol Biol. 2019 Aug;26(8):720-

Insert

LN-3xFlag-SV40NLS

Use

Lamdan entry vector with nls to generate tetherin…

Tags

LN-3xFlag-SV40NLS

Expression

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry.3xFLAG.NOS1APC20-WPRE

Plasmid

#127866

Purpose

pAAV plasmid expressing an mCherry.3xFLAG.NOS1APC20 fusion protein under the hSyn promoter

Depositor

Florian Freudenberg

Article

Candemir et al Eur Neuropsychopharmacol. 2016 Apr;26(4)

Insert

Nos1ap (Nos1ap Mouse)

Use

AAV

Tags

3xFLAG and mCherry

Expression

Mutation

C-terminal 20 amino acids only

Promoter

hSyn

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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