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pCu-His6-Atg13[571–700]-Ss(424)

Plasmid

#166852

Purpose

Expresses 6xHis tagged ATG13 [571–700] fragment with 14 scramble amino acid sequence DIKLIDTVDLESCN under a Cu promoter in yeast.

Depositor

Daniel Klionsky

Article

Gatica et al Autophagy. 2020 Jun;16(6):1007-1020. doi

Insert

Atg13[571–700] (ATG13 Budding Yeast)

Use

Tags

6xHis

Expression

Yeast

Mutation

Promoter

pCu

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B

Plasmid

#163907

Purpose

Full length mouse KIF13B for purification from insect cells

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Use

Tags

Cleavable 6 x HIS tag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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KIF13B 1-584

Plasmid

#163908

Purpose

Motile fragment of mouse KIF13B (aa 1-584) for purification from insect cells.

Depositor

Martin Loose

Article

Duellberg et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

Insert

kif13b (Kif13b Mouse)

Use

Tags

Cleavable 6 x HIS tag

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Ir7a-T2A-QF2 HDR plasmid

Plasmid

#140943

Purpose

Plasmid for CRISPR-generated knock-in line that expresses a QF2 transcriptional activator by knocking-in a T2A-QF2 fragment into the coding sequence of the endogenous Aedes aegypti Ir7a gene

Depositor

Leslie Vosshall

Article

Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

Insert

Ir7a-left-HDR-arm; T2A-QF2-SV40; 3xP3-dsRed-SV40; Ir7a-right-HDR-arm

Use

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-CreERT2 sgAdam10 v3

Plasmid

#158047

Purpose

lenti-viral construct with tamoxifen inducible Cre recombinase and U6 driven sgRNA against mouse Adam10. NO Cas9

Depositor

Daniel Schramek

Article

Loganathan et al Science. 2020 Mar 13;367(6483):1264-1269

Insert

Adam10 sgRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 5, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCS2 Halo-Tev- HSPB7 (zebrafish) C117S

Plasmid

#108915

Purpose

Express C117S mutant of Zebrafish Halo-Tev-HSPB7 in mammalian cells

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

Halo-TEV-HSPB7

Use

Tags

Halo-TEV

Expression

Mammalian

Mutation

Cysteine 117 to Serine

Promoter

SP6

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

Plasmid

#133791

Purpose

U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018

Depositor

Benjamin Kleinstiver, Joseph Bondy-Denomy

Article

Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

Insert

St1Cas9 sgRNA entry vector

Use

Tags

Expression

Mammalian

Mutation

V1 sgRNA architecture from Carter et al. biorxiv …

Promoter

U6

Available since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pPN432

Plasmid

#137870

Purpose

Expression of gRNA a3 targeting TCF4 for CRISPRa; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPN431

Plasmid

#137869

Purpose

Expression of gRNA a2 targeting TCF4 for CRISPRa; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPN435

Plasmid

#137867

Purpose

Expression of gRNA i3 targeting TCF4 for CRISPRi; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPN434

Plasmid

#137866

Purpose

Expression of gRNA i2 targeting TCF4 for CRISPRi; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pPN459

Plasmid

#137875

Purpose

PiggyBac vector for expression of 1x gRNA targeting TCF4 for CRISPRi; mRFP-T2A-BlasticidinR

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPN446

Plasmid

#137868

Purpose

Expression of gRNA a1 targeting TCF4 for CRISPRa; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pPN454

Plasmid

#137865

Purpose

Expression of gRNA i1 targeting TCF4 for CRISPRi; U6 promoter

Depositor

Lindy Barrett

Article

Hazelbaker et al Sci Rep. 2020 Jan 20;10(1):635. doi: 10.

Insert

TCF4 (TCF4 Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pSD32_pCAG-PV1-FKBP-L1-TP901-324C-NLS-BGHpA

Plasmid

#108812

Purpose

Expresses aa324 to C-terminus of TP901 integrase with N-term FKBP and C-term NLS

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

TP901 integrase fragment aa324 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2665_pCAG-PV1-FlpO-N27-L1-nMag-NLS-BGHpA

Plasmid

#108825

Purpose

Expresses N-terminus to aa27 of Flp recombinase fused to nMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa27

Use

Cre/Lox and Synthetic Biology

Tags

nMag,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2666_pCAG-PV1-pMag-L1-FlpO-28C-NLS-BGHpA

Plasmid

#108826

Purpose

Expresses aa28 to C-terminus of Flp recombinase fused to pMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa28 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and pMag

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2669_pCAG-PV1-FlpO-N168-L1-nMag-NLS-BGHpA

Plasmid

#108827

Purpose

Expresses N-terminus to aa168 of Flp recombinase fused to nMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa168

Use

Cre/Lox and Synthetic Biology

Tags

nMag,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2670_pCAG-PV1-pMag-L1-FlpO-169C-NLS-BGHpA

Plasmid

#108828

Purpose

Expresses aa169 to C-terminus of Flp recombinase fused to pMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa169 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and pMag

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2673_pCAG-PV1-FlpO-N374-L1-nMag-NLS-BGHpA

Plasmid

#108829

Purpose

Expresses N-terminus to aa374 of Flp recombinase fused to nMag CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa374

Use

Cre/Lox and Synthetic Biology

Tags

nMag,NLS

Expression

Mammalian

Mutation

Promoter

pCAG

Available since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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