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Plasmid#65777PurposeHuman expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNADepositorInsertSpCas9 gRNA backbone, without spacer sequence
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
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anti-FLAG M2 light chain
Plasmid#175358PurposeMammalian expression vector for over-expression of anti-FLAG M2 light chainDepositorInsertanti-FLAG M2 light chain
UseTagsN-terminal secretion signal peptide (MEAPAQLLFLLL…ExpressionMammalianMutationPromoterCMVAvailable sinceOct. 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVX-SRRM2-mCherry
Plasmid#174089PurposeInducibly expresses SRRM2-mCherry in mammalian cells with the Tet-on systemDepositorInsertSRRM2 (SRRM2 Human)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterTREtightAvailable sinceSept. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
SpyCatcher003-sfGFP
Plasmid#133449PurposeExpresses SpyCatcher003-superfolder GFP in bacterial cytoplasmDepositorInsertSpyCatcher003-sfGFP
UseTagsHis6 and TEV siteExpressionBacterialMutationPromoterT5Available sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-pCMV-PfV-GS-Sapphire
Plasmid#116933PurposeMammalian vector for expression of the 40nm GEMDepositorInsertPfv
UseTagsSapphireExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pKAM-BFP-Puro
Plasmid#101864PurposeLentiviral empty backbone, dual BFP and puro markersDepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EFS-EGFP-T2A-Bsd
Plasmid#193198PurposeLentiviral vector expressing EGFP (driven by EFS promoter) and a blasticidin resistance markerDepositorInsertEGFP
UseLentiviralTagsExpressionMammalianMutationPromoterEFSAvailable sinceDec. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-p21
Plasmid#171122PurposeDoxycycline inducible expression of p21DepositorInsertp21 (CDKN1A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP TEV LIC cloning vector (1M)
Plasmid#29656DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHis6-MBP-TEVExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only