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Plasmid#146355PurposeInsect Expression of DmXRN1_1-573DepositorInsertDmXRN1_1-573 (pcm Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-Dm4EHP-A_G
Plasmid#146357PurposeInsect Expression of Dm4EHP-ADepositorInsertDm4EHP-A (4EHP Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-DEST47-Noa1-GFP
Plasmid#191968PurposeGateway Destination Vector, containing NOA1-GFP chimera coding sequence, for Gateway Cloning TechnologyDepositorInsertNoa1-GFP
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-TRP1-pCUP1-2xphlO
Plasmid#191794PurposeYeast gene induction with copper promoterDepositorInsertTRP1 pCUP1 with 2 phlO sites
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRESS-mScarletI-antiFRET-P2A2-linker_noSacI
Plasmid#191473PurposeNegative control for ratiometric comparison between green fluorescent proteins and sensorsDepositorInsertnone
UseTagsHis-mScarletI-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDRESS-mTq2-antiFRET-P2A2-linker_noSacI
Plasmid#191472PurposeNegative control for ratiometric comparison between red fluorescent proteins and sensorsDepositorInsertnone
UseTagsHis-mTurquoise2-antiFRETlinker-P2AExpressionBacterial and MammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
3xnls-G-Ca-FLITS
Plasmid#191463PurposeGenetically encoded calcium sensor (GECI) G-Ca-FLITS that reports with a lifetime changeDepositorInsertG-Ca-FLITS
UseTags3xNLSExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HseIF2g_2-472_AF
Plasmid#148685PurposeMammalian Expression of HseIF2g_2-472DepositorInsertHseIF2g_2-472
UseTagsExpressionMammalianMutationone silent mutation compared to the sequence give…PromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-HsYTHDF2_1-385_AB
Plasmid#148320PurposeMammalian Expression of HsYTHDF2_1-385DepositorInsertHsYTHDF2_1-385 (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pT7-MS2-HA-C1-HsYTHDF2_386-579_AB
Plasmid#148321PurposeMammalian Expression of HsYTHDF2_386-579DepositorInsertHsYTHDF2_386-579 (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pU-YLL058W-lt
Plasmid#176564PurposeExpression of YLL058W for Lanchea thermotolerans for auxotrophic selection in the absence of uracilDepositorInsertYLL058W-lt
UseTagsExpressionYeastMutationPromoterTDH1(GAP)Available sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-Spe4-RfA-Snaptag-stop
Plasmid#186395PurposeDestination vector for the expression of a fluorescent fusion of a protein of interest with C-ter Snap tag into embryos, by mRNA microinjectionDepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceOct. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBF120*
Plasmid#162852PurposeExpression of PilB F120 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationF120AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBT125*
Plasmid#162853PurposeExpression of PilB T125 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationT125AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBN189*
Plasmid#162854PurposeExpression of PilB N189 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationN189AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBL193*
Plasmid#162855PurposeExpression of PilB L193 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationL193AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBK197*
Plasmid#162856PurposeExpression of PilB K197 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationK197AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBQ231*
Plasmid#162857PurposeExpression of PilB Q231 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationQ231AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBL240*
Plasmid#162858PurposeExpression of PilB L240 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationL240AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUCP20 PilBS249*
Plasmid#162859PurposeExpression of PilB S249 Amber with a C-terminal histagDepositorInsertPilB
UseTagsHistidine tag (H6x)ExpressionBacterialMutationS249AmberPromoterAvailable sinceOct. 3, 2022AvailabilityAcademic Institutions and Nonprofits only