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Plasmid#69923PurposeExpresses mutant myc-RNF114 in mammalian cellsDepositorInsertRNF114 (RNF114 Human)
UseTagsExpressionMammalianMutationS224PPromoterCMVAvailable sinceFeb. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV Puro SCRIB P305L
Plasmid#88887PurposeSCRIB - Proline to Lysine mutation at 305 a.a. in MSCV backboneDepositorInsertSCRIB (SCRIB Human)
UseRetroviralTagsExpressionMutationProline to Leucine mutation @ 305th amino acidPromoterMSCV promoterAvailable sinceMarch 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA_DiCre_60.F2
Plasmid#60208PurposeExpresses a fusion protein of FRB-linker peptide 2- Cre(60-343)DepositorInsertCre recombinase (CD207 Bacteriophage P1, Synthetic, Human)
UseTagsFRAP and Nuclear localisation signalExpressionMammalianMutationamino acids 2021-2112 of FRAP with a T2098L mutat…PromoterCMVAvailable sinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
SERPINE2-bio-His
Plasmid#53416PurposeExpresses full-length Glia-derived nexin precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSERPINE2 (SERPINE2 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
Dynamin-I-K44A-6His/Flag
Plasmid#32696DepositorInsertDynamin I (Dnm1 Rat)
UseTagsFlag and His6ExpressionMammalianMutationK44APromoterCMVAvailable sinceNov. 18, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLX302-MKP7-V5 puro
Plasmid#87771PurposeExpresses V5 tagged human MAPK phosphatase 7 (MKP7/DUSP16)DepositorInsertMKP7 (DUSP16 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-ORF8
Plasmid#165089Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 ORF8 (ORF8 SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 10, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
tdminiSOG-H2B-C-10
Plasmid#57804PurposeLocalization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528DepositorInsertH2B (H2BC11 Human)
UseTagstdminiSOGExpressionMammalianMutationD26G and V119I in H2BPromoterCMVAvailable sinceJan. 29, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFastBacDual with LFn + FlaAAA
Plasmid#84867PurposeBaculovirus Expression of LFn-FlaAAA fusion. This is a negative control for LFn FlaA in which the 3 C-terminal leucines have been replaced by alanine.DepositorInsertsLFn
FlaAAA
UseBaculovirusTags6xHisExpressionInsectMutation3 C-terminal leucines have been replaced by alani…PromoterPolyhedrinAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pST_SERF2_HUMAN (pBS0751)
Plasmid#185239PurposeFor the mammalian expression of the human protein SERF2_HUMAN. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertSERF2_HUMAN
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
GAL-AR-1-503
Plasmid#89093Purposemammalian expression of human androgen receptor N-terminal 1-503 amino acids linked to GAL4 DNA binding domainDepositorInserthAR aa 1-503 (AR Human)
UseTagsGAL4 DBDExpressionMammalianMutationPromoterAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
PhyB-mCherry-Spc72
Plasmid#51582PurposeConstitutively express spindle pole body localized PhyB in Saccharomyces cerevisiaeDepositorInsertPhyB (PHYB Mustard Weed)
UseTagsSpc72 and mCherryExpressionYeastMutation1-908aa onlyPromoterADH1prAvailable sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDH-PD-L1 3SA/4NQ
Plasmid#121491PurposeExpression of human PD-L1 3SA/4NQ with PD-L1 shRNADepositorInsertPD-L1
UseLentiviralTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
SERPINC1-bio-His
Plasmid#52076PurposeExpresses full-length Antithrombin-III precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSERPINC1 (SERPINC1 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAR-L26A,F27A
Plasmid#89084Purposemammalian expression of human androgen receptor with mutation of N-terminal FxxLF motifDepositorInserthAR FxxLF mutant (AR Human)
UseTagsExpressionMammalianMutationhAR-L26A,F27APromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
AKT-APEX2-P2A-mKate2
Plasmid#67654PurposeMammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). Also contains bicistronially expressed cytoplasmic red reporter.DepositorInsertAKT-PH-APEX2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pST_MAHS_RAMVA (pBS0310)
Plasmid#185169PurposeFor the mammalian expression of the tardigrade protein MAHS_RAMVA. This is one of the top performing targets in our apoptosis assay (it protected from apoptosis).DepositorInsertMAHS_RAMVA
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2e[Δa10, Δ18a, Δ24a, 31a, 37b, 46]
Plasmid#26570DepositorInsertcalcium channel alpha-1B subunit (Cacna1b Rat)
UseTags6XHis and V5ExpressionMammalianMutationDeletion of exons a10, 18a, and 24a. Contains …PromoterAvailable sinceNov. 29, 2010AvailabilityAcademic Institutions and Nonprofits only -
p3E-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75177PurposeNuclear mCerulean3 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with C-terminal fusions.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pKI-cjFOXL2-coemiRFP670-PGK-Puro
Plasmid#186168PurposeKnock-in gene targeting in marmoset cells at FOXL2 locusDepositorInsertemiRFP670
UseMarmoset targetingTagsExpressionMutationhuman codon-optimizedPromoterAvailable sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only