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pENTR-p38KTRClover

Plasmid

#59142

Purpose

ORF encoding p38 KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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PL6-CS-WR

Plasmid

#122512

Purpose

construction for sgRNA transcription with Plasmodium falciparum U6 promotor

Depositor

Lubin Jiang

Article

Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

Type

Empty backbone

Use

CRISPR

Tags

Expression

Mutation

Promoter

PfU6

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFX-mito-eqFP650

Plasmid

#174179

Purpose

Express eqFP650 with mitochondria-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

eqFP650 with mitochondria-targeting sequence

Use

Tags

the mitochondria-targeting sequence of the cytoch…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-GalT-mCherry

Plasmid

#174473

Purpose

Express mCherry with golgi apparatus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mCherry with golgi apparatus-targeting sequence

Use

Tags

β1,4-galactose transferase

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-H2B-SECFP

Plasmid

#175838

Purpose

Express SECFP with nucleus-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with nucleus-targeting sequence

Use

Tags

H2B (human histone 2B)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS2-QFGal4-SV40pA

Plasmid

#155126

Purpose

In vitro transcription of QFGal4 mRNA

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

QFGal4

Use

Expression in zebrafish

Tags

Expression

Mammalian

Mutation

Promoter

SP6

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta2

Plasmid

#101290

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

1-727 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-tTA

Plasmid

#193338

Purpose

AAV-mediated expression of tTA under the CAG promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

tTA2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-mROCK1-delta1

Plasmid

#101289

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

1-375 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pmCellFree_KA1 ZIKV E

Plasmid

#145341

Purpose

Cell-free/mammalian expression vector for Zika virus Envelope protein with N-term eGFP

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Envelope

Use

Tags

8xHis and eGFP

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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Human SERCA3 (pMT2)

Plasmid

#75189

Purpose

Mammalian expression of ATP2A3

Depositor

David MacLennan, Jonathan Lytton

Article

Poch et al Am J Physiol. 1998 Dec;275(6 Pt 1):C1449

Insert

ATP2A3 (ATP2A3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

adenovirus major late promoter

Available since

June 21, 2016

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP[N150TAA]

Plasmid

#164581

Purpose

superfolder GFP with N150TAA mutation

Depositor

Ryan Mehl

Article

Bednar et al ACS Chem Biol. 2021 Nov 19;16(11):2612-2

Insert

sfGFP-150TAA

Use

Tags

6xHis

Expression

Bacterial

Mutation

N150TAA Mutation

Promoter

T7

Available since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV3Tag8li-hSAPS1-HA

Plasmid

#165466

Purpose

Human SAPS1 mammalian expression vector with C-terminal HA tag.

Depositor

Martin Dorf

Article

Li et al Proc Natl Acad Sci U S A. 2014 May 13;11

Insert

Protein phosphatase 6 regulatory subunit 1 (PPP6R1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN mEGFP

Plasmid

#188643

Purpose

This is the Lazarou lab's standard retrovirus vector backbone for expression of mEGFP-tag fusions onto the N-terminus of cloned genes

Depositor

Michael Lazarou

Article

Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

Type

Empty backbone

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-Beclin1

Plasmid

#99574

Purpose

Expressed Cas9 with sgRNA targeting Beclin1

Depositor

Edward Campbell

Article

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting Beclin1 (BECN1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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ORAI1-hBACCS2

Plasmid

#67626

Purpose

Mammalian expression of ORAI1-hBACCS2, a fusion protein of ORAI1 and human blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

ORAI1-hBACCS2

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 mSmoothened WT

Plasmid

#126408

Purpose

Full length mouse Smoothened cDNA. Expression of Smoothened in mammalian cell lines.

Depositor

Stacey Ogden

Article

Marada et al PLoS Genet. 2015 Aug 20;11(8):e1005473.

Insert

m-Smoothened (Smo Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 13, 2019

Availability

Academic Institutions and Nonprofits only

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p-G4PID

Plasmid

#209638

Purpose

Expression G4PID in mammalian cells

Depositor

Zhou Xiang

Article

Lu et al Nucleic Acids Res. 2024 Mar 7:gkae126. d

Insert

G4PID (DHX36 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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AWP-031

Plasmid

#213967

Purpose

Expresses dPb2Cas12a under an IPTG-inducible promoter and contains a cassette for constitutive CRISPR array expression.

Depositor

Alexander Westermann

Article

Prezza et al Proc Natl Acad Sci U S A. 2024 Feb 6;121

Insert

Cas12a (cas12a Segatella bryantii, Synthetic)

Use

Tags

3XFLAG

Expression

Bacterial

Mutation

D875A to inactivate target nuclease activity

Promoter

PcfxA, IPTG inducible

Available since

April 5, 2024

Availability

Academic Institutions and Nonprofits only

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pAIP

Plasmid

#74171

Purpose

Lentiviral vector for expression of heterologous genes

Depositor

Jeremy Luban

Article

Pertel et al Nature. 2011 Apr 21;472(7343):361-5. doi

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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