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pILGFPH7

Plasmid

#83554

Purpose

Used to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.

Depositor

Claudia Vickers

Article

Peng et al Microb Cell Fact. 2015 Jun 26;14:91. doi

Insert

ADH2 promoter-yEGFP

Use

Tags

Expression

Yeast

Mutation

Promoter

ADH2

Available since

Dec. 15, 2016

Availability

Academic Institutions and Nonprofits only

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pET21-pelB-LaG-18

Plasmid

#172763

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-18

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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Vh-HA-FKBP

Plasmid

#20220

Depositor

Carolyn Bertozzi

Article

Czlapinski et al J Am Chem Soc. 2008 Oct 8. 130(40):13186

Insert

FKBP (FKBP1A Human)

Use

Tags

HA and Vh

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2009

Availability

Academic Institutions and Nonprofits only
pET21-pelB-LaG-42

Plasmid

#172758

Purpose

Bacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.

Depositor

Michael Rout

Article

Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

Insert

LaG-42

Use

Tags

6xHIS and pelB leader for periplasmic secretion

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 18, 2021

Availability

Academic Institutions and Nonprofits only

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PDZD7-TwinStrepII

Plasmid

#83198

Purpose

Expression of tagged protein in mammalian cells

Depositor

Peter Barr-Gillespie

Article

Morgan et al Elife. 2016 Aug 15;5:e18312. doi: 10.755

Insert

PDZD7 (Pdzd7 Mouse)

Use

Tags

Twin Strep Tag II

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pFRT/FLAG/HA-DEST EIF2C3

Plasmid

#19889

Depositor

Thomas Tuschl

Article

Landthaler et al RNA. 2008 Oct 31. ():.

Insert

Eukaryotic translation initiation factor 2C, 3 (AGO3 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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pCDH-Flag-mPRMT7-GFP

Plasmid

#87104

Purpose

Express mouse PRMT7 with Flag-tag in mammalian cells

Depositor

Min Gyu Lee

Article

Lee et al Nucleic Acids Res. 2016 Dec 15;44(22):10

Insert

Protein arginine methyltransferase 7 (Prmt7 Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

EF1

Available since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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CAV1-deltaN-mEGFP

Plasmid

#27707

Depositor

Ari Helenius

Article

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Insert

caveolin-1, N-terminal truncated (60-178) (CAV1 Canis lupus familiaris)

Use

Tags

monomeric EGFP

Expression

Mammalian

Mutation

N-terminal truncation with AA 1-59 missing. The r…

Promoter

Available since

July 28, 2011

Availability

Academic Institutions and Nonprofits only

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pLD-puro-2A-rtTA-TcVA

Plasmid

#24592

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 23, 2010

Availability

Academic Institutions and Nonprofits only

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pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS

Plasmid

#199350

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP-SUMOstar

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP

Plasmid

#199361

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

3C-SUMOstar-EGFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP

Plasmid

#199362

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

TEV-SUMOstar-EGFP

Expression

Mammalian

Mutation

WT

Promoter

Available since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pETM6-RgTALsyn

Plasmid

#100947

Purpose

Expression of Tyrosine Ammonia Lyase from inducible T7 promoter

Depositor

Mattheos Koffas

Article

Jones et al MBio. 2017 Jun 6;8(3). pii: e00621-17. d

Insert

RgTALsyn

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Silent mutation to remove internal NdeI site on T…

Promoter

T7

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Flag-MBD1 R44Q-14-mVenusC

Plasmid

#191394

Purpose

R44Q binding pocket mutant of MBD domain of MBD1 fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

pcDNA3 Flag-MBD1 R44Q-14-mVenusC

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

R44Q

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA3-V5-PTPN14-del-N

Plasmid

#61004

Purpose

Expresses human PTPN14 (del-FERM domain mutant) in mammalian cells, N-terminus V5 tag

Depositor

Jianmin Zhang

Article

Wilson et al J Biol Chem. 2014 Aug 22;289(34):23693-7

Insert

PTPN14 delete N (PTPN14 Human)

Use

Tags

V5 tag

Expression

Mammalian

Mutation

deleted amino acids 17-310

Promoter

CMV

Available since

Nov. 20, 2014

Availability

Academic Institutions and Nonprofits only
pHH0103_PACSIN2-1/1

Plasmid

#91330

Purpose

Protein expression and purification of human SH3 domain construct PACSIN2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

PACSIN2-1/1 (PACSIN2 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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Flag Nprl2 pLJM1

Plasmid

#46338

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Nprl2 (NPRL2 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
F-CDK8L

Plasmid

#24762

Depositor

Joan Conaway, Ronald Conaway

Article

Conaway et al FEBS Lett. 2005 Feb 7. 579(4):904-8.

Insert

F-CDK8L (CDK19 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 14, 2010

Availability

Academic Institutions and Nonprofits only
pscALPS SIV-MND2 vpx

Plasmid

#115811

Purpose

Encodes codon optimized 3xFLAG-SIVMND2 Vpx (AY159322) and puromycin resistance protein

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

SIVMND2 Vpx (AY159322)

Use

Lentiviral

Tags

3x-FLAG

Expression

Mutation

Promoter

SFFV

Available since

Jan. 3, 2019

Availability

Academic Institutions and Nonprofits only

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p10xUAS-luc2

Plasmid

#173006

Purpose

firefly luciferase reporter vector with 10x clustered UAS binding sites

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

10xUAS-luc2

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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