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Plasmid#194683PurposeCMV driven expression of the calcium recorder Caprola_05 in mammalian cell lines fused to mEGFPDepositorInsertCaprola_05-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO APEX2-GFP
Plasmid#129640PurposeExpress APEX2-GFP fusion in mammalian cellsDepositorInsertAPEX2-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAV Ef1a-EGFP-WPRE
Plasmid#135428PurposeCan be used to express EGFP. Can also be used to create adeno-associated virus for delivery of the EGFP sequenceDepositorInsertEGFP
UseAAVTagsExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT_CMV_NES-Caprola_on-mEGFP
Plasmid#194686PurposeCMV driven expression of the consitutively active calcium recorder positive control Caprola_on in mammalian cell lines fused to mEGFPDepositorInsertCaprola_on-mEGFP
UseTagsmEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-moxGFP-Puro-MAPRE1
Plasmid#207794PurposeDonor template to insert moxGFP-2A-Puro into the C-terminus of the MAPRE1 locus for growing microtubule tip visualization. To be co-transfected with sgRNA plasmid px330-PITCh-MAPRE1 Addgene #207793DepositorArticleInsertMAPRE1 Homology Arms flanking a moxGFP-Puro Cassette (MAPRE1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceDec. 1, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pT7-EGFP-C1-HsYTHDF1_365-559_AB
Plasmid#148309PurposeMammalian Expression of HsYTHDF1_365-559DepositorInsertHsYTHDF1_365-559 (YTHDF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-EGFP-C1-HsYTHDF2_1-385_AB
Plasmid#148311PurposeMammalian Expression of HsYTHDF2_1-385DepositorInsertHsYTHDF2_1-385 (YTHDF2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
Str-KDEL_TNF-SBP-EGFP
Plasmid#65278Purposesynchronize trafficking of TNF from the ER (RUSH system)DepositorInsertTNF (TNF Human)
UseTagsEGFP and Streptavidin Binding Protein (SBP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAF0033 Ef1a-Pa01-2a-EGFP
Plasmid#193460PurposeExpression of RecombinaseDepositorInsertPa01
UseTagsExpressionMammalianMutationPromoterEf1aAvailable sinceNov. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBR322-Pdp1_NTD-GFP-HiBiT
Plasmid#198317PurposeLoads HiBiT-tagged GFP into PVCs using Pdp1 packaging domain; also contains PnfDepositorInsertPdp1_NTD-GFP-HiBiT
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP II (pMIG II)
Plasmid#52107Purposea bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFPDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseRetroviralTagsIRES-EGFPExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMRX-IB-pSu9-HaloTag7-mGFP
Plasmid#184905PurposeStable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activityDepositorInsertPresequence of Fo-ATPase subunit 9
UseTagsHaloTag7 and monomeric EGFPExpressionMammalianMutationPromoterAvailable sinceJune 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xMma PylT_GFP 150TAG 6xHis
Plasmid#140015PurposePlasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integrationDepositorInsertsfGFP
UseTags6xHisExpressionMammalianMutation150TAGPromoterEF1Available sinceJune 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
TetOn-mCherry-eGFP-RAMP4
Plasmid#109014PurposeTet-On construct to induce expression of tandem eGFP-mCherry tagged RAMP4 (ER marker)DepositorInsertRAMP4 (SERP1 Human)
UseLentiviralTagsmCherry-eGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-PdCO-EGFP-WPRE
Plasmid#198513PurposeExpresses optimized PdCO in frame with EGFP under control of human synapsin1 promotor.DepositorHas ServiceAAV1 and AAV5InsertPdCO
UseAAVTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-FKBP-GFP-HIS3MX6
Plasmid#183023PurposePCR-based tagging of yeastDepositorInsertFKBP
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
FU-H2B-GFP-IRES-Puro
Plasmid#69550PurposeLentiviral, selectable fluorescent GFP tagging of nuclei.DepositorInsertHistone 2B - green fluorescent protein fusion
UseLentiviralTagsGFP fusionExpressionMutationPromoterAvailable sinceApril 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
DL016:CMV::eGFP-iPAK4
Plasmid#177881PurposeeGFP-IPAK4 fusion with CMV promoter, cloned into a lentivirus backboneDepositorInsertiPAK4
UseLentiviralTagseGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-GFP-Sox11
Plasmid#120387Purposeover expression of sox11 using infectionDepositorInsertsox11 (Sox11 Mouse)
UseLentiviralTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Pur-CAG-EGFP
Plasmid#80945Purposehuman AAVS1 site targeting donor plasmid for knocking-in EGFP expression cassette driven by CAG promoterDepositorInsertEGFP
UseTagsExpressionMammalianMutationPromoterCAG promoterAvailable sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only