We narrowed to 8,695 results for: sgRNA

1. pDGE792

   Plasmid

   #167300

   Purpose

   Vector for plant genome editing containing pRPS5a:GFP-Cas9 expression cassette, Bar (BASTA/PPT) selection marker, and FCY-UPP and Ca-Bs3 counterselction markers, but not sgRNAs.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDGE687

   Plasmid

   #167299

   Purpose

   Vector for plant genome editing containing p35S:GFP-Cas9 expression cassette, nptII selection marker, and SlFAST2 and Ca-Bs3 counterselction markers, but not sgRNAs.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Plant

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. PLJR965

   Plasmid

   #115163

   Purpose

   Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

   Depositor

   Sarah Fortune

   Article

   Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

   Inserts

   Sth1 sgRNA scaffold
   Sth1 dCas9
   TetR (deleted TetON) MtbCO
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. hAAVS1-gs4_EF1a-Cas9-2A-GFP

   Plasmid

   #118414

   Purpose

   CRISPR knockout, expresses Cas9, and sgRNA targeting AAVS1 locus

   Depositor

   Mark Denham

   Article

   Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

   Insert

   gRNA AAVS1 (AAVS1 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. EF1a-dCas9-KRAB-GFP backbone

   Plasmid

   #194281

   Purpose

   Vector for CRISPRi-GFP expression ready for gateway cloning of sgRNA

   Depositor

   Elizabeth Kirby

   Article

   Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

   Insert

   dCas9-KRAB-GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   GFP

   Promoter

   EF1a

   Available Since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. BB3cK_pGAP_23*_pPFK300_Cas9

   Plasmid

   #104910

   Purpose

   hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   April 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. BB3cN_pGAP_23*_pLAT1_Cas9

   Plasmid

   #104906

   Purpose

   hCas9 under control of LAT1 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC

   Depositor

   Brigitte Gasser

   Article

   Gassler et al Methods Mol Biol. 2019;1923:211-225. doi

   Inserts

   HH - FS23 linker - HDV
   human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Feb. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. LentiCRISPR v2-sgLKB1 clone1

   Plasmid

   #162125

   Purpose

   Lentiviral sgRNA plasmid targeting human LKB1

   Depositor

   Boyi Gan

   Article

   Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

   Insert

   sgLKB1 (STK11 Human)
   

   Use

   Lentiviral

   Available Since

   Jan. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pU6-sgGFP-NT1

   Plasmid

   #46914

   Purpose

   Human pSico-based U6 vector containing murine U6 promoter and sgRNA targeting GFP (NT1)

   Depositor

   Stanley Qi, Jonathan Weissman

   Article

   Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

   Insert

   sgGFP-NT1
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Oct. 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPR-E

    Plasmid

    #78852

    Purpose

    Introduce sgRNA into a lentiviral vector (LentiCRISPR V2) which contains eSpCas9 and puromycin cassette

    Depositor

    Phillip Abbosh

    Article

    Sikder et al Mol Cancer Res. 2020 Dec 3. pii: 1541-77

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Cas9-P2A-Puro

    Expression

    Mammalian

    Available Since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. PLJR962

    Plasmid

    #115162

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. LentiCRISPR v2-sgLKB1 clone2

    Plasmid

    #162126

    Purpose

    Lentiviral sgRNA plasmid targeting human LKB1

    Depositor

    Boyi Gan

    Article

    Lee et al Nat Cell Biol. 2020 Feb;22(2):225-234. d

    Insert

    sgLKB1 (STK11 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. Lenti-eCas9

    Plasmid

    #140237

    Purpose

    Lentiviral vector for expression of high-specificity eSpCas9(1.1) and sgRNA scaffold

    Depositor

    Urs Greber

    Article

    Bauer et al Cell Rep. 2019 Dec 17;29(12):3785-3795.e

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    EF-1a core

    Available Since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLV.5’-LMNA-eGFP-KI

    Plasmid

    #170545

    Purpose

    Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP flanked by homology arms for LMNA

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for LMNA knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid

    #227327

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCutamp

    Plasmid

    #140632

    Purpose

    Plasmid-curing in Escherichia coli by targeting the AmpR promoter

    Depositor

    Sheng Yang

    Article

    Zhang et al ACS Synth Biol. 2020 Jun 17. doi: 10.102

    Insert

    SpCas9_lambda-RED system, SacB, Rha induction system, sgRNA targeting AmpR promoter
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. SlugCas9

    Plasmid

    #163793

    Purpose

    Expresses SlugCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al Nucleic Acids Res. 2021 Mar 15. pii: 617

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. qTAG-N-Solo-mScarlet-TUBA1B

    Plasmid

    #207764

    Purpose

    Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCRISPR-cBEST

    Plasmid

    #125689

    Purpose

    C to T base editor for actinomycetes

    Depositor

    Tilmann Weber

    Article

    Tong et al Proc Natl Acad Sci U S A. 2019 Oct 8;116

    Insert

    streptomyces codon optimized spCas9n (D10A), modified sgRNA casette, streptomyces codon optimized rAPOBEC1
    

    Use

    CRISPR and Synthetic Biology

    Promoter

    ermE*/tipA

    Available Since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. LCV2 V-1

    Plasmid

    #84804

    Purpose

    Cas9 with sgRNA targeting Exon 1 of VEGF-A

    Depositor

    Glenn Yiu

    Article

    Yiu et al Invest Ophthalmol Vis Sci. 2016 Oct 1;57

    Insert

    gRNA targeting VEGF-A (VEGFA Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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