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Plasmid#163885PurposeAcute Depletion P300-EYFPDepositorInsertP300 (Ep300 Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
BoxB-MS2 adRNA (RAB7A-ACG site)
Plasmid#170155PurposeAAV vector carrying a BoxB-MS2 adRNA targeting the RAB7A transcript for C-U editingDepositorInsertBoxB-RAB7A(ACG)-MS2
UseAAVTagsExpressionMammalianMutationPromoterHuman U6Available sinceMarch 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
PD-1-miSFIT-Ce67
Plasmid#124680PurposeTuning expression of PD-1DepositorInsertPD-1 (PDCD1 Human)
UseTagsExpressionMammalianMutationPromoterminimal CMVAvailable sinceJune 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGAD-Atg17
Plasmid#121386PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
UseTagsExpressionYeastMutationPromoterADH1 promoterAvailable sinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Flag TSC2 (T1462A)
Plasmid#14130DepositorInsertTSC2 T1462A (TSC2 Human)
UseTagsFlagExpressionMammalianMutationT1462APromoterAvailable sinceMarch 2, 2007AvailabilityAcademic Institutions and Nonprofits only -
pSmart HC Kan-U6-Nla-repeat-BbsI-Nla-repeat
Plasmid#178883PurposeVector for cloning Nla-IC-CRISPR RNA for expression in mammalian cells.DepositorInsertU6-Nla repeat-BbsI-Nla repeat
UseTagsExpressionMammalianMutationPromoterU6Available sinceJan. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(H75S)
Plasmid#137828PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75S mutant TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(H75S)
UseTags3x FLAGExpressionMammalianMutationMutated Histidine 75 to Serine in NTEVp fragmentPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
A3Bi-Cas9n-UGI
Plasmid#119135PurposeBase editor made from full-length APOBEC3B with an L1 intron to prevent self-restrictionDepositorInsertAPOBEC3B L1 intron-Cas9 nickase-UGI
UseCRISPRTagsExpressionMutationL1 intron insertionPromoterCMVAvailable sinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CMV-Sa-SauriCas9
Plasmid#135967PurposeExpresses Sa-SauriCas9, and cloning backbone for sgRNADepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-V5-PTPN14-PPxY
Plasmid#61006PurposeExpresses human PTPN14 (PPxY domain mutant) in mammalian cells, N-terminus V5 tagDepositorInsertPTPN14 PPxY mutation (PTPN14 Human)
UseTagsV5 tagExpressionMammalianMutationPPxY mutationPromoterCMVAvailable sinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH7
Plasmid#83554PurposeUsed to evaluate the expression output of ADH2 promoter with yEGFP used as the reporter gene.DepositorInsertADH2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterADH2Available sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-42
Plasmid#172758PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-42; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-42
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDH-Flag-mPRMT7-GFP
Plasmid#87104PurposeExpress mouse PRMT7 with Flag-tag in mammalian cellsDepositorInsertProtein arginine methyltransferase 7 (Prmt7 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterEF1Available sinceMay 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET21-pelB-LaG-18
Plasmid#172763PurposeBacterial expression of HIS-tagged anti-GFP nanobody LaG-18; HIS tag preceded by HRV 3C/PreScission cleavage site.DepositorInsertLaG-18
UseTags6xHIS and pelB leader for periplasmic secretionExpressionBacterialMutationPromoterT7Available sinceAug. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PDZD7-TwinStrepII
Plasmid#83198PurposeExpression of tagged protein in mammalian cellsDepositorInsertPDZD7 (Pdzd7 Mouse)
UseTagsTwin Strep Tag IIExpressionMammalianMutationPromoterCMVAvailable sinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Vh-HA-FKBP
Plasmid#20220DepositorInsertFKBP (FKBP1A Human)
UseTagsHA and VhExpressionMammalianMutationPromoterAvailable sinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTS97 pHAGE2 CMVtetO2 GFP-22xGS-SUMOstar-MCS
Plasmid#199350PurposeLentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOstar-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsEGFP-SUMOstarExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS108 pHAGE2 CMVtetO2 MCS-3C-SUMOstar-22xGS-GFP
Plasmid#199361PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally 3C-SUMOstar-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTags3C-SUMOstar-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTS109 pHAGE2 CMVtetO2 MCS-TEV-SUMOstar-22xGS-GFP
Plasmid#199362PurposeLentiviral transfer plasmid for doxycycline inducible expression of a C-terminally TEV-SUMOstar-GFP-tagged protein in mammalian cells.DepositorTypeEmpty backboneUseLentiviralTagsTEV-SUMOstar-EGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
CAV1-deltaN-mEGFP
Plasmid#27707DepositorInsertcaveolin-1, N-terminal truncated (60-178) (CAV1 Canis lupus familiaris)
UseTagsmonomeric EGFPExpressionMammalianMutationN-terminal truncation with AA 1-59 missing. The r…PromoterAvailable sinceJuly 28, 2011AvailabilityAcademic Institutions and Nonprofits only