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Plasmid#196486PurposeTo express Methanosarcina mazei PylRS(Y384F Y306A) and allow UAG codon to be translated into Z-Lys or Azido-Z-Lys when used together with the pU6tRNAPyl plasmid.DepositorInsertpyrrolysyl-tRNA synthetase variant with Y384F Y306A mutations
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pClathrin-miRFP718nano
Plasmid#195747PurposeClathrin labelled with near-infrared fluorescent protein miRFP718nanoDepositorInsertmiRFP718nano-clathrin light chain
UseTagsmiRFP718nanoExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTubulin-miRFP718nano
Plasmid#195746PurposeHuman alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nanoDepositorInsertmiRFP718nano-human alpha-tubulin
UseTagsmiRFP718nanoExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_rSERT_C109A_Y107C
Plasmid#190174PurposeFor expression of a SERT mutant in mammalian cells and measuring accessibility in the SERT extracellular pathwayDepositorInsertSLC6A4 Serotonin Transporter (Slc6a4 Rat)
UseTagsFLAG, His6, and c-mycExpressionMammalianMutationchanged Cys109 to alanine, changed tyr107 to cyst…PromoterCMVAvailable sinceDec. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-E (aa1-138)-EGFP
Plasmid#187697PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa1-138PromoterCMVAvailable sinceSept. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-G (aa1-372)-EGFP
Plasmid#187699PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa1-372PromoterCMVAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-F (aa373-564)-EGFP
Plasmid#187698PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa373-564PromoterCMVAvailable sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-C (aa139-371)-EGFP
Plasmid#187695PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa139-371PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-A (aa139-564)-EGFP
Plasmid#187693PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa139-564PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-D (aa160-340)-EGFP
Plasmid#187696PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa160-340PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-Zyxin-B (aa309-564)-EGFP
Plasmid#187694PurposeVisualization of ZyxinDepositorInsertZyxin (Zyx Mouse)
UseLentiviralTagsEGFPExpressionMammalianMutationaa309-564PromoterCMVAvailable sinceSept. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-TR-del1-40
Plasmid#180236PurposeExpresses T and R domains of Colicin E1 with N-terminal 40 residue deletion (residues 41-364)DepositorInsertColicin E1
UseTags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T and R domains (r…PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-T-P110A
Plasmid#180237PurposeExpresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110DepositorInsertColicin E1
UseTags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T domain of ColE1 …PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
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ppBAalbNixE1E3E4 PUb-YFP
Plasmid#173666Purposetransgenesis vector for Aedes albopictus ectopic expression of the Nix geneDepositorInsertsNix masculinisation gene
YFP
UseTagsSV40 NLSExpressionInsectMutationNix isoforms with exons E1, E3, E4PromoterAedes aegypti PUb and ownAvailable sinceMay 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
CPL2g/pDONR221
Plasmid#172093PurposeGenomic fragment containing the upstream region and coding region of CER11/CPL2 cloned into pDONR221DepositorInsertCPL2 (CPL2 Mustard Weed)
UseGateway entry vectorTagsExpressionMutationPromoterCPL2Available sinceOct. 20, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR221-kcnj12a
Plasmid#173141PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj12a (kcnj12a Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj16
Plasmid#173146PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj16a (kcnj16 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationStop codon removedPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-kcnj19b
Plasmid#173149PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj19b (kcnj19b Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR-D-kcnj1a.1
Plasmid#173125PurposeThis gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.DepositorInsertkcnj1a (kcnj1a.1 Zebrafish)
UsePcr cloning vectorTagsExpressionMutationPromoterNo promoter at 5 end; T7 promoter at 3 end.Available sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only