Addgene

We narrowed to 27,535 results for: eng

Showing: 7201 - 7220 of 27535 results

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pBS Wnt-1HA

Plasmid

#17367

Depositor

Jan Kitajewski

Article

Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

Insert

Wnt-1HA (Wnt1 Mouse)

Use

Tags

HA

Expression

Bacterial

Mutation

Promoter

Available since

July 7, 2008

Availability

Academic Institutions and Nonprofits only
pB1H2w5-12-C81S

Plasmid

#18043

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Insert

HhaI C81S mutant

Use

Tags

Expression

Bacterial

Mutation

Simply used as an insert between Kpn1 and Xba1

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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pGT-S2

Plasmid

#122583

Purpose

MAGIC donor plasmid (RSF1010 oriR with beta-lactamase/sfGFP payload)

Depositor

Harris Wang

Article

Ronda et al Nat Methods. 2019 Feb;16(2):167-170. doi

Insert

beta-lactamase/sfGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pKmK.I2R

Plasmid

#125064

Purpose

Storage plasmid for K.marxianus right homology arm

Depositor

John Morrissey

Article

Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

Insert

Right homology arm for targeting integration to chromosome V: 23743..21744

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 29, 2019

Availability

Academic Institutions and Nonprofits only

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p4xEGFP-N1

Plasmid

#122164

Purpose

Expression of 4xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

4xEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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p8xEGFP-N1

Plasmid

#122168

Purpose

Expression of 8xEGFP

Depositor

Georg Mayr

Article

Bohm et al Anal Biochem. 2017 Sep 15;533:48-55. doi

Insert

8xEGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pB1H2w2-12-C81S

Plasmid

#18042

Depositor

Scot Wolfe

Article

Noyes et al Nucleic Acids Res. 2008 Mar 10. ():.

Insert

HhaI C81S mutant

Use

Tags

Expression

Bacterial

Mutation

Simply used as an insert to drop out between Kpn1…

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only

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Cas9m2

Plasmid

#47314

Purpose

Cas9 D10A+H840A

Depositor

George Church

Article

Mali et al Nat Biotechnol. 2013 Aug 1. doi: 10.1038

Insert

Cas9m2

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
HEK3_pegF_60bp

Plasmid

#174856

Purpose

Express forward pegRNA mediating 991-bp deletion and 60-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegF_60bp insertion

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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HEK3_pegR_60bp

Plasmid

#174857

Purpose

Express reverse pegRNA mediating 991-bp deletion and 60-bp insertion

Depositor

Wen Xue

Article

Jiang et al Nat Biotechnol. 2021 Oct 14. pii: 10.103

Insert

HEK3_pegR_60bp insertion

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pLSLGFP

Plasmid

#51494

Purpose

pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

GFP
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS and flag

Expression

Mutation

Promoter

virion-sense LIR and 2x35S

Available since

May 1, 2014

Availability

Academic Institutions and Nonprofits only

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pGD250

Plasmid

#132371

Purpose

contains the sequence of mClover3 for C-term fusions in the pGGD000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

linker-mClover3

Use

Greengate entry plasmid

Tags

Expression

Mutation

Promoter

Available since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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promoter-less mRFP1E

Plasmid

#183144

Purpose

BioBrick pSB1C3 plasmid encoding a promoter-less version of mRFP1E

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

RBS, mRFP1E

Use

Synthetic Biology

Tags

Expression

Mutation

BioBrick sites removed

Promoter

Available since

Sept. 1, 2022

Availability

Academic Institutions and Nonprofits only

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BDS-2 3x WT (p53 binding site)

Plasmid

#16515

Depositor

Bert Vogelstein

Article

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

p53 binding site (SFN Human)

Use

Tags

Luciferase

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only
pCfB4170

Plasmid

#126750

Purpose

Expresses pTEF2-mKate2-tIDP1 and pTRX2_5xUAS-yEGFP-tCYC1 in yeast from plasdmid pRS413

Depositor

Michael Jensen

Article

Zhang et al ACS Synth Biol. 2016 Dec 16;5(12):1546-1

Insert

yEGFP

Use

Tags

Expression

Mutation

Promoter

pTRX2_5xUAS

Available since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pCS4083

Plasmid

#131739

Purpose

Two Color Control Assay bicistronic BFP-mCherry-sTRSVctl

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

sTRSV

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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PSD95-TS:YSOG2

Plasmid

#42227

Depositor

Roger Tsien

Article

Butko et al Nat Neurosci. 2012 Nov;15(12):1742-51. d

Insert

PSD95

Use

Tags

TS:YSOG2

Expression

Mammalian

Mutation

Promoter

Available since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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InterTag-BFP

Plasmid

#186901

Purpose

Cell-free expression of transmembrane protein PDGFR-tagBFP-sfCherry11-SpyTag

Depositor

Allen Liu, Taekjip Ha

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

SpyTag003-sfCherry11-tagBFP-PDGFR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB8739

Plasmid

#126905

Purpose

Template for PCR amplification of XdCrtYB

Depositor

Irina Borodina

Article

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

XdCrtYB

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pEA183

Plasmid

#187873

Purpose

Expresses eGFP under the virB promoter.

Depositor

Kan Wang

Article

Aliu et al Plant Biotechnol J. 2022 Jun 11. doi: 10

Insert

eGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Agrobacterium virB promoter

Available since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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