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Showing: 7181 - 7200 of 7946 results

  2. pWT046e

    Plasmid
    #107900
    Purpose
    W2.5-1 plasmid. Contains PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C) and is part of CAMERA 2.5
    Depositor
    David Liu
    Article
    Tang et al Science. 2018 Feb 15. pii: science.aap89
    Insert
    PTetO-SD8-BE2, PBAD-sgRNA5 (sgRNA3-A8C)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    107900-plasmid-map-sequence-id-208438

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  3. OA-1127B

    Plasmid
    #190997
    Purpose
    Expresses arrays of gRNA targeting rel1 promoter under U6b/c promoter
    Depositor
    Omar Akbari
    Article
    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.
    Insert
    U6b:sgRNArel1A1 - U6c:sgRNArel1A2 - U6b:sgRNArel1A3 - U6c:sgRNArel1A4
    Use
    Tags
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Oct. 18, 2022
    Availability
    Academic Institutions and Nonprofits only
    190997-plasmid-map-sequence-id-374622

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  16. pHEE401

    Plasmid
    #71286
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    sgRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…
    Available since
    Jan. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    71286-plasmid-map-sequence-id-127777

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  17. pU6-(BsaI)_CBh-UN-Cas9

    Plasmid
    #135011
    Purpose
    Expression vector for sgRNAs cloned into the BsaI sites and for expression of Cas9 with 126aa MRN-recruiting domain from HSV-1 UL12 fused to N-terminus of Cas9
    Depositor
    Yosef Shaul
    Article
    Reuven et al Biomolecules. 2019 Oct 8;9(10). pii: bio
    Insert
    humanized S. pyogenes Cas9
    Use
    Tags
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Expression
    Mammalian
    Mutation
    126aa domain from HSV-1 UL12 fused to the N-termi…
    Promoter
    CBh
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    135011-plasmid-map-sequence-id-267594

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  18. Cas9/pTREX-n

    Plasmid
    #68708
    Purpose
    Expresses the fusion gene CAS9-HA-2xNLS-GFP in the pTREX-n backbone. This vector is used for cloning a specific sgRNA by BamHI, to be co-expressed with Cas9 for genome editing in Trypanosoma cruzi.
    Depositor
    Roberto Docampo
    Article
    Lander et al MBio. 2015 Jul 21;6(4). pii: e01012-15.
    Insert
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 (Addgene) .
    Use
    CRISPR
    Tags
    Fusion gene Cas9-HA-2xNLS-GFP from vector pMJ920 …
    Expression
    Mutation
    C4239T mutation that eliminates a BamHI restricti…
    Promoter
    Available since
    Sept. 14, 2015
    Availability
    Academic Institutions and Nonprofits only
    68708-plasmid-map-sequence-id-123989

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Showing: 7181 - 7200 of 7946 results