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Plasmid#166531PurposescFv of a human scaffold targeting Glycyl-tRNA synthetase. Antigen coverage aa 55-739 of 739DepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
L-WARS-26
Plasmid#166528PurposescFv of a human scaffold targeting Tryptophanyl-tRNA synthetase. Antigen coverage aa 1-1471 of 471, full-lengthDepositorInsertsingle chain-Fv, scFv
UseRecombinant antibody expressionTags3xFLAG, His6 and OmpA leader sequenceExpressionBacterialMutationPromoterLacZAvailable sinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-hAR-F23A
Plasmid#89089Purposemammalian expression of human androgen receptor with mutation of N-terminal FxxLF motifDepositorInserthAR-FxxLF mutant (AR Human)
UseTagsExpressionMammalianMutationhAR-F23APromoterCMVAvailable sinceApril 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT TO GFP MPS1 K-R
Plasmid#59819PurposeAllows the integration of GFP MPS1 K-R in the genome and Tet-inducible expression.DepositorInsertMps1 (TTK Human)
UseTagsEmGFPExpressionMammalianMutationKD, catalytically inactive, D664APromoterhybrid human cytomegalovirus (CMV)/TetO2 promoterAvailable sinceFeb. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
Plxnb2(L)-Fc-His
Plasmid#72128PurposeExpresses the extracellular region of the PlexinB2 protein (ie, long), C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnb2 (Plxnb2 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-Strep/HA-UBE2Q2
Plasmid#124668PurposeExpresses UBE2Q2 with N-terminal Strep-HA tag in mammalian cellsDepositorInsertUBE2Q2 (UBE2Q2 Human)
UseTags2xStrep/HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
Cntn4.2-AP-His
Plasmid#71942PurposeExpresses the entire Contactin 2, isoform 2 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertCntn4.2 (Cntn4 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MtPylRS (human-opti)
Plasmid#164080PurposeTo express a Methanosarcina thermophila derived PylRS in mammalian cells for genetic code expansionDepositorInsertMtPylRS-478(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-MfBulKRS (human-opti)
Plasmid#164196PurposeTo express a Methanosarcina flavescens derived BulKRS in mammalian cells for genetic code expansionDepositorInsertMf BulKRS(human-opti)
UseSynthetic BiologyTagsExpressionMammalianMutationY269A;Y347FPromoterCMVAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
Neo1.h-AP-His
Plasmid#71970PurposeExpresses the extracellular region of the Neogenin 1, isoform h protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorInsertNeo1.h (Neo1 Mouse)
UseTagsAP-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB119
Plasmid#68573PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterCauliflower mosaic virus 35S promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAM-AAV-hSncg-EGFP
Plasmid#153198PurposeHuman gamma-synuclein (hSncg) promoter-mediates gene expression in retinal neurons with fluorescent reporterDepositorTypeEmpty backboneUseAAVTagsExpressionMammalianMutationPromoterAvailable sinceJuly 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNAS1b split mCherry NEDD4-2 WW2
Plasmid#111883PurposeMode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)DepositorTypeEmpty backboneUseTagsSplit mCherry system: NEDD4-2 WW2 fused to C-term…ExpressionBacterialMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
Plxnc1-Fc-His
Plasmid#72131PurposeExpresses the extracellular region of the PlexinC1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.DepositorInsertPlxnc1 (Plxnc1 Mouse)
UseTagsFc-HisExpressionMammalianMutationPromoterCMVAvailable sinceMarch 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLX-B3omega2 (GB1695)
Plasmid#160644PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (omega2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX-B2alpha2 (GB1696)
Plasmid#160620PurposepLX series: pBBR1-based T-DNA binary vector for plant transformation (alpha2)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMpGWB121
Plasmid#68575PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterMpEF1alpha promoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAD-CMV-STAT5A-DN-CMV-GFP
Plasmid#83252PurposeAdenoviral expression of STAT5A dominant negative with co-expression of GFPDepositorInsertsUseAdenoviralTagsExpressionMutationC-terminal deletion of 135 nucleotides (dominant …PromoterCMVAvailable sinceSept. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
PKM-IRES-hrGFP
Plasmid#73405PurposeMammalian expression of mouse PKM and hrGFPDepositorInsertPKM (Prkcz Mouse)
UseTags3xFLAG and pIRES-hrGFP-2aExpressionMammalianMutationtruncated, constitutively active formPromoterCMVAvailable sinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pOCC254
Plasmid#118889Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal mCherry, cleavable with TEVDepositorInsertNcoI-HIS6-MBP-3C-mCherry-TEV-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease; mCherry, cl…ExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only