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FB398
Plasmid#203634PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertFB367+FB395
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_84
Plasmid#60076PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_83
Plasmid#60075PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_82
Plasmid#60074PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
RFN_EGFP_80
Plasmid#60072PurposeExpresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nucleaseDepositorInsertpair of multiplex gRNAs targeting EGFP
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceOct. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
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MSP680
Plasmid#65772PurposeHuman expression vector for SpCas9 EQR variant: CMV-T7-humanSpCas9(D1135E/R1335Q/T1337R)-NLS-3xFLAG (EQR variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135E/R1335Q/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135E, R1335Q, and T1337R mutations in Cas9PromoterCMV & T7Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPEE47
Plasmid#139426PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.DepositorInsertmNeonGreen
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPEE46
Plasmid#139425PurposeRecyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mRuby3 ORF and a flexible linker at the 5' end.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mGrn-myc
Plasmid#178944PurposeConstruct for expressing murine progranulin (Grn) in AAVDepositorInsertGrn (Grn Mouse)
UseAAVTagsMycExpressionMutationPromoterCBAAvailable sinceOct. 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
MSP469
Plasmid#65771PurposeHuman expression vector for SpCas9 VQR variant: CMV-T7-humanSpCas9(D1135V/R1335Q/T1337R)-NLS-3xFLAG (VQR variant)DepositorInsertmammalian codon-optimized Streptococcus pyogenes Cas9 (D1135V/R1335Q/T1337R)-NLS-3XFlag
UseCRISPRTags3x FLAG and NLSExpressionMammalianMutationD1135V, R1335Q, and T1337R mutations in Cas9PromoterCMV & T7Available sinceJune 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDR348 FANCF
Plasmid#47510Purposehuman gRNA expression vector targeting FANCFDepositorInsertgRNA-FANCF (FANCF Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pC011 LwCas13a locus with targeting spacer
Plasmid#91903PurposeLwCas13a locus into pACYC184 with targeting spacerDepositorInsertLwCas13a with targeting spacer
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBp-FGFR3c-WT
Plasmid#45711DepositorInsertFGFR3 IIIc (FGFR3 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
QUAS-jGCaMP7s-T2A-tdTomato
Plasmid#200010PurposeQUAS reporter line expressing GCaMP7s calcium indicator and tdTomato, separated by T2ADepositorInsert3xP3-ECFP-jGCaMP7s-td-Tomato
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWKS7
Plasmid#139414PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised mRuby3 marker.DepositorInsertmRuby3
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pC009 LshCas13a locus with targeting spacer
Plasmid#91900PurposeLshCas13a locus into pACYC184 with targeting spacerDepositorInsertLshCas13a locus with targeting spacer
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 16, 2017AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGWKS10
Plasmid#139417PurposeFluoroescent marker for use in Cryptococcus neoformans. Contains a codon optimised Clover marker.DepositorInsertClover
UseTagsExpressionYeastMutationPromoterTEF1Available sinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only
