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Plasmid#223898Purpose4G-cloning donor for C-terminal PK6 tagDepositorInsertPK6
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_FLAG
Plasmid#223895Purpose4G-cloning donor for C-terminal FLAG tagDepositorInsertFLAG
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_HALO
Plasmid#223899Purpose4G-cloning donor for C-terminal HALO tagDepositorInsertHALO
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6
Plasmid#223871Purpose4G-cloning donor for N-terminal His6 tagDepositorInsertHis6
UseGolden-gate donorTags6xHisExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_HALO
Plasmid#223885Purpose4G-cloning donor for N-terminal HALO tagDepositorInsertHALO
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_GFP
Plasmid#223880Purpose4G-cloning donor for N-terminal GFP tagDepositorInsertGFP
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_His8
Plasmid#223886Purpose4G-cloning donor for C-terminal His8 tagDepositorInsertHis8
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Ct)_TS
Plasmid#223891Purpose4G-cloning donor for C-terminal TwinStrep tagDepositorInsertTwinStrep
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-R26-FLEX-mStr
Plasmid#135619PurposeDonor vector for genomic targeting of a CAG-FLEX-mStrawberry cassette to the mouse Rosa26 locusDepositorInsertCAG-FLEX-mStrawberry flanked by Rosa26 homology arms (Gt(ROSA)26Sor Mouse)
UseAAV, CRISPR, and Mouse TargetingTagsExpressionMutationPromoterAvailable sinceMay 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBlue-ACTB-IR-IRES-Puro-pEF1α-EGFP-IR-ACTB
Plasmid#169921PurposeDNA donor plasmid for site-specific integration of IRES-Puro-pEF1a-EGFP cassette at the ACTB locus using Cas9-transposase fusion proteins.DepositorInsertIRES-Puro-pEF1a-EGFP
UseCRISPRTagsExpressionMutationPromoterEF1aAvailable sinceJune 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEF1a_BbsI-gRNA scaffold-EC73-5'-EcoRI-EC73-3'-HDV_pA_pCMV_EGFP-L202S-P2A-mCherry_pA
Plasmid#176461PurposeVector for constructing retron Ec73ncRNA-gRNA chimeric RNA (Ec73 rgRNA), inserting spacer sequence by BbsI digestion and inserting donor sequence by EcoRI digestion. EGFP(L202S)-P2A-mCherry driven by CMVDepositorInsertBbsI-gRNA scaffold-Ec73ncRNA-EcoRI-HDV
UseCRISPRTagsExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pXZ13lacZalpha
Plasmid#160229Purposeplaces LacZalpha unit between Esp3I sites for better cutting of plasmid and ability to distinguish recombinants as white colonies when plated on Xgal platesDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterlacAvailable sinceOct. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
Mouse 5' Srcap AID GFP Puro
Plasmid#127903PurposeHDR (donor) Plasmid for Inserting PuroR-2A-GFP-AID into the 5' end of the Mouse Srcap GeneDepositorInsertSrcap (Srcap Mouse, Mustard Weed)
UseDonor plasmidTagsGFP, AID, PuroRExpressionMutationPromoterAvailable sinceSept. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pD(Gib)_3'omega
Plasmid#223870Purpose4G-cloning donor for 3'-Gibson overhang, omega-sequenceDepositorInsert3'omega sequence and spacer
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-TEV
Plasmid#223872Purpose4G-cloning donor for N-terminal His6-TEV tagDepositorInsertHis6-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-3C
Plasmid#223873Purpose4G-cloning donor for N-terminal His6-3C tagDepositorInsertHis6-3C
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-FLAG3
Plasmid#223874Purpose4G-cloning donor for N-terminal His6-FLAG3 tagDepositorInsertHis6-FLAG3
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_ph
Plasmid#223857Purpose4G-cloning donor for insect promoter and terminator, no tag addedDepositorInsertpolyhedrin promoter and SV40 polyA
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_CMV
Plasmid#223860Purpose4G-cloning donor for mammalian promoter and terminator, no tag addedDepositorInsertCMV promoter and bGH polyA
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(P+T)_T7
Plasmid#223854Purpose4G-cloning donor for bacterial promoter and terminator, no tag addedDepositorInsertT7 promoter and terminator
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only