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Showing: 701 - 720 of 24268 results
  1. pKAN43Ag8-GFP

    Plasmid
    #178182
    Purpose
    Plant binary vector expressing GFP by a enhanced 35S promoter
    Depositor
    Insert
    EGFP (eGFP )
    Use
    Tags
    Expression
    Plant
    Mutation
    The CAT is inserted after the start codon.
    Promoter
    The minimal 35S promoter from Cauliflower Mosaic …
    Available since
    Sept. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
  2. pDONR221-SLC7A1_STOP

    Plasmid
    #161091
    Purpose
    Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.
    Insert
    SLC7A1 (SLC7A1 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 21, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
  3. pT7CFE1-Nhis-GST-CHA-YTH domain (YTHDC1, 317-532)

    Plasmid
    #102277
    Purpose
    For expression of His-GST-tagged human YTHDC1 YTH domain using mammalian cell-free protein expression system (1-step human high-yield mini IVT kit, cat. #88890, Thermo Fisher Scientific)
    Depositor
    Insert
    YTH domain of human YTHDC1 (317-532) (YTHDC1 Human)
    Use
    For mammalian cell-free protein expression system…
    Tags
    GST, HA, and HIS
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 12, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. CECR2

    Plasmid
    #39066
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Insert
    CECR2 (CECR2 Human)
    Use
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Promoter
    Available since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only
  5. pattB-aubergine-ADH-gf

    Plasmid
    #69448
    Purpose
    genomic fragment of Drosophila aubergine was cloned from w1 background and inserted into pattB plasmid; mutagenesis PCR was used to change the catalytic residue DDH to ADH to express cat-dead Aub
    Depositor
    Insert
    aubergine (aub Fly)
    Use
    Tags
    NA
    Expression
    Insect
    Mutation
    chr2L:10,998,626(−)T>G
    Promoter
    aubergine
    Available since
    Oct. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
  6. pET29b-RupA_C1-A1-T1-CHis

    Plasmid
    #109255
    Purpose
    NRPS module containing CAT domains with C-terminal 6His tag
    Depositor
    Insert
    RupA_C1-A1-T1
    Use
    Tags
    His tag and S-Tag
    Expression
    Bacterial
    Mutation
    contains aa 1..1025 of WP_015523502.1
    Promoter
    T7
    Available since
    July 6, 2018
    Availability
    Academic Institutions and Nonprofits only
  7. pSEVA3b61

    Plasmid
    #58310
    Purpose
    Shuttle vector: pUC ori, pTHT15 ori for use in E. coli/B. subtilis. Cannot modify orientation of cat gene; promoter too weak in E. coli, probably transcribed via repB promoter in bicistronic manner.
    Depositor
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    July 29, 2015
    Availability
    Academic Institutions and Nonprofits only
  8. pSAP

    Plasmid
    #206429
    Purpose
    A vector backbone domesticated for SapI (pCC1BAC-based), contains elements for selection, maintenance, and propagation in S. cerevisiae (TRP1) and E. coli (cat).
    Depositor
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Promoter
    Available since
    Jan. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
  9. pG5ElbLuc

    Plasmid
    #89341
    Purpose
    The plasmid pG5ElbLuc was prepared from pG5E1bCAT by replacing the CAT gene (EcoRI and Sty I fragment) with the firefly luciferase gene (from pGEM-Luc) as a 1745bp StuI-Hind111 fragment.
    Depositor
    Type
    Empty backbone
    Use
    Luciferase
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 16, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 701 - 720 of 24268 results