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Plasmid#140221PurposeConstitutive PGK driven expression of N terminal dCpf1 (Cas12a) 406-split half with leucine zipperDepositorInsertdLbCpf1 (Cas12a) N terminal half (406) with zipper
UseTagsExpressionMammalianMutationPromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R364
Plasmid#138234PurposeExpresses the split mCherry reporter interrupted by the Cth-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R274
Plasmid#138223PurposeExpresses the split mCherry reporter interrupted by the TvoVMA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:GFP1–9
Plasmid#108187PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (GFP1-9) in plantsDepositorInsertGFP1-9
UseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R269
Plasmid#138181PurposeExpresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R272
Plasmid#138221PurposeExpresses the split mCherry reporter interrupted by the SspGyrB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R401
Plasmid#138222PurposeExpresses the split mCherry reporter interrupted by the TerThyX artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R360
Plasmid#138230PurposeExpresses the split mCherry reporter interrupted by the Ama-Ter artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
CMVTO-p450-FRB-cTEVP
Plasmid#179676PurposeER-associated FRB split-cTEVP (Rapalog inducible)DepositorInsertFRB (MTOR Human, Synthetic)
UseTagscTEVPExpressionMammalianMutationPromoterAvailable sinceMarch 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-S11-DI-GFP1–9
Plasmid#108260PurposeBinary vectors used for the inducible expression of dual intein-coupled tripartite split-sfGFP components in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-cMSPdH45
Plasmid#199767PurposeExpresses split-intein fused MSPdH45 for circularization during expression (internal His-Tag)DepositorInsertcMSPdH45
UseTagsHis6-TagExpressionBacterialMutationinternal His-TagPromoterAvailable sinceJuly 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFP.R275
Plasmid#138224PurposeExpresses the split mCherry reporter interrupted by the PhoRadA intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
nGR2175-gs10-NLS-VPR
Plasmid#188264PurposePlasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminusDepositorInsertnGR2175
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-NGFP-VMAn-FKBP
Plasmid#70225PurposeExpresses N-terminal portion of GFP along with VMA intein for protein splicingDepositorInserteGFP
UseTagsFK506- and rapamycin-binding protein (FKBP), Link…ExpressionMammalianMutationChanged Glu 125 to Ile, Deleted amino acids 129-2…PromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FRB-VMAc-CGFP
Plasmid#70226PurposeExpresses C-terminal portion of GFP along with VMA intein for protein splicingDepositorInserteGFP
UseTagsFKBP-rapamycin binding (FRB) domain of the mammal…ExpressionMammalianMutationChanged Ile 129 to Cys, Deleted amino acids 1-128PromoterCMVAvailable sinceNov. 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:(MCS)-3xHA-S11
Plasmid#108180PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFP.R371
Plasmid#138241PurposeExpresses the split mCherry reporter interrupted by the MP-M-DnaB artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
UBQ10:sXVE:S10-(MCS)-3xHA
Plasmid#108178PurposeBinary vectors used for the inducible expression of tripartite split-sfGFP components (with a 3xHA tag) in plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionPlantMutationPromoterUBQ10:sXVE:Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FKBP-CD28TMD-NTEVp(WT)
Plasmid#137826PurposeMESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal WT TEV proteaseDepositorInsertFKBP-CD28TMD-NTEVp(WT)
UseTags3x FLAGExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
plIVMD156
Plasmid#160934PurposeIn vivo mRNA display Gateway Destination constructDepositorInsertMCP-Gateway cloning site-HIS tag, 3'UTR MS2 Stem Loop
UseTagsHIS tag, 3'UTR MS2 Stem LoopExpressionYeastMutationPromoterMET25Available sinceJan. 7, 2021AvailabilityAcademic Institutions and Nonprofits only