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pDZ416 (24PP7SL loxP-Kan-loxP)

Plasmid

#45163

Depositor

Robert Singer, Daniel Zenklusen

Article

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

24xPP7 Stem Loop Cassette

Use

Cre/Lox

Tags

Expression

Mutation

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only

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pSmart-Kan-HC-EF1a-Nla-Cas8-7-5-11

Plasmid

#193752

Purpose

human codon optimized Nla-cas8c-Cas7c-Cas5c-Cas11c cloned into pSmart-HC-Kan-EF1a-bGH

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-Cas7c-Cas5c-Cas11c-bGH PolyA

Use

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-sfGFP-kanMX (pMaM17)

Plasmid

#173452

Purpose

Contains mCherry-sfGFP tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Khmelinskii et al Nat Biotechnol. 2012 Jun 24;30(7):708-14

Insert

mCherry-sfGFP-ADH1ter

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGZ108 (pFA6a-3FLAG-MNase-kanMX6)

Plasmid

#70231

Purpose

ChEC-tagging vector encoding a short linker and aa 83-231 of Mnase in pFA6a-3HA-kanMX6 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Use

Yeast genomic targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1 KanR foundation lox casette

Plasmid

#132388

Purpose

Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

Depositor

Gerold Schmitt-Ulms

Article

Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

Insert

G418 resistance

Use

CRISPR and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

promoterless

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

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BsaI-tagRFP-BsaI-pUC-kan

Plasmid

#170771

Purpose

test plasmid for Golden Gate assembly, encodes fluorescent red protein

Depositor

Ichiro Matsumura

Article

Matsumura et al Biotechniques. 2022 May;72(5):185-193. d

Insert

tagRFP

Use

Tags

Expression

Mutation

Promoter

none

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pFA6a-mCherry-mNeonGreen-kanMX (pKBJ006)

Plasmid

#173453

Purpose

Contains mCherry-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mCherry-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-His-tag-kanMX6

Plasmid

#20763

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Tags

Expression

Mutation

Promoter

Available since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-H-mNeonGreen-kanMX (pKBJ009)

Plasmid

#173456

Purpose

Contains mScarletH-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-H-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-I-mNeonGreen-kanMX (pKBJ007)

Plasmid

#173454

Purpose

Contains mScarletI-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-I-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBS-SKII-3XHA-fSNAP-Kan

Plasmid

#188918

Purpose

Integration cassette to create C-terminal fusions for fluorescent tagging

Depositor

Stephen Buratowski

Article

Baek et al G3 (Bethesda). 2022 Aug 9. pii: 6659097.

Insert

3XHA-fSNAP-Kan

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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M4754 KanMX::HIS3 Disruptor Converter

Plasmid

#51684

Purpose

Yeast marker swap plasmid for converting KanMX3 to HIS3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

HIS3 (HIS3 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-mScarlet-mNeonGreen-kanMX (pKBJ008)

Plasmid

#173455

Purpose

Contains mScarlet-mNeonGreen tandem fluorescent protein timer

Depositor

Anton Khmelinskii

Article

Fung et al Methods Mol Biol. 2022;2378:85-100. doi:

Insert

mScarlet-mNeonGreen

Use

Tags

Expression

Yeast

Mutation

Promoter

no promoter

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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M3925 trp1::KanMX3 Disruptor Converter

Plasmid

#51681

Purpose

Yeast marker swap plasmid for converting Trp1 to KanMX3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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M4755 KanMX::LEU2 Disruptor Converter

Plasmid

#51685

Purpose

Yeast marker swap plasmid for converting KanMX3 to LEU2

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

LEU2 (LEU2 Budding Yeast)

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 6, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPA-mCherry-Kan

Plasmid

#44950

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable mCherry with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 16, 2013

Availability

Academic Institutions and Nonprofits only

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M3927 ura3::KanMX3 Disruptor Converter

Plasmid

#51682

Purpose

Yeast marker swap plasmid for converting URA3 to KanMX3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX-tetO-Pcyc1-GFP

Plasmid

#41025

Depositor

Michael Nick Boddy

Article

Zilio et al Yeast. 2012 Aug 29. doi: 10.1002/yea.292

Insert

tetO7-Pcyc1-GFP

Use

N-terminal tagging/promoter replacement

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2012

Availability

Academic Institutions and Nonprofits only

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M3929 his3::KanMX3 Disruptor Converter

Plasmid

#51679

Purpose

Yeast marker swap plasmid for converting HIS3 to KanMX3

Depositor

David Stillman

Article

Voth et al Yeast. 2003 Aug;20(11):985-93.

Insert

KanMX3

Use

Yeast marker swap

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-Kan

Plasmid

#44943

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

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