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6x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64392

Purpose

encodes six a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
pHSG-PCNA3

Plasmid

#66127

Purpose

S. solfataricus PCNA3

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

Insert

DNA polymerase sliding clamp 3 (pcn Sulfolobus solfataricus P2)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pHSG-PCNA2

Plasmid

#66126

Purpose

S. solfataricus PCNA2

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Chembiochem. 2010 Jul 26;11(11):1517-20.

Insert

DNA polymerase sliding clamp 2 (SSO_RS05185 Sulfolobus solfataricus P2)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pGEX Shark-tSH2

Plasmid

#126672

Purpose

Expression of Shark tandem SH2 domains SNAP tagged for use as FRET reporter

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

Shark tandem SH2 domains

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 12, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-M

Plasmid

#1128

Depositor

Susan Lindquist

Article

Scheibel et al Nat Struct Biol 2001 Nov;8(11):958-62.

Insert

M (SUP35 Budding Yeast)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
370 pTol2-lmo2:CherryDest

Plasmid

#73489

Purpose

Tol2 vector with lmo2 promoter upstream of Cherry, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

lmo2 promoter:mCherry-attR1-attR2

Use

Tags

Expression

Mutation

Promoter

Available since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRL-TK 4x mut G

Plasmid

#11320

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation G (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
PGTE+mU6-WT

Plasmid

#12116

Depositor

Inder Verma

Article

Tiscornia et al Proc Natl Acad Sci U S A. 2004 May 11. 1

Insert

mU6 promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 27, 2007

Availability

Academic Institutions and Nonprofits only
3x_a1gRNAOp_pCYC1m_yeBFP2

Plasmid

#64391

Purpose

encodes three a1 operator sites upstream of minimal pCYC1 promoter driving yeast enhanced BFP

Depositor

Timothy Lu

Article

Farzadfard et al ACS Synth Biol. 2013 Sep 11.

Insert

yeast enhanced BFP2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 12, 2015

Availability

Academic Institutions and Nonprofits only
SCP2-pUC119

Plasmid

#21951

Depositor

James Kadonaga

Article

Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

Insert

SCP2 (super core promoter 2)-pUC119

Use

A puc derivative - a cloning vector

Tags

Expression

Mutation

Promoter

Available since

Feb. 19, 2010

Availability

Academic Institutions and Nonprofits only

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pRB306

Plasmid

#8666

Depositor

David Botstein

Article

Schatz et al Mol Cell Biol 1986 Nov;6(11):3722-33.

Insert

TUB1 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
M50-IRES-GFP-IRES-tauLacZ LNL TV

Plasmid

#15646

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):817-31.

Insert

M50-IRES-GFP-IRES-tauLacZ LNL TV

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pRB2070

Plasmid

#8671

Depositor

David Botstein

Article

Richards et al Mol Biol Cell 2000 May;11(5):1887-903.

Insert

TUB1 with LYS2 (TUB1 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 30, 2005

Availability

Academic Institutions and Nonprofits only
pRL-TK 4x mut I

Plasmid

#11322

Depositor

Phil Sharp

Article

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

4 bulged binding sites for CXCR4 siRNA antisense

Use

Luciferase

Tags

Rr-luc

Expression

Mutation

Mutation I (see paper) on inner 2 binding sites.

Promoter

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
OE2-M71

Plasmid

#15630

Depositor

Peter Mombaerts

Article

Feinstein et al Cell. 2004 Jun 11. 117(6):833-46.

Insert

OE2-M71-IRES-tauGFP ACNF TV

Use

High copy number

Tags

Expression

Mutation

Promoter

Available since

Aug. 24, 2007

Availability

Academic Institutions and Nonprofits only
pCF

Plasmid

#216326

Purpose

PUlaA promoter driving expression of sfgfp

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

Superfolder green fluorescent protein

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pUlaR

Plasmid

#216327

Purpose

T7 promoter driving expression of ulaR

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

L-Ascorbate-6-P-responsive transciptional repressor

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pBad_UlaABC

Plasmid

#216328

Purpose

Arabinose-inducible PBad promoter driving expression of ulaA, ulaB, and ulaC

Depositor

Mark Styczynski

Article

Piorino et al ACS Synth Biol. 2022 Nov 18;11(11):3592-

Insert

UlaABC

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228i16

Plasmid

#209738

Purpose

ColA BBa_B0014 tetAp SD8 G1_PylRS-[mCNPR43] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

G1PylRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAB228i24

Plasmid

#209739

Purpose

ColA BBa_B0014 tetAp SD8 G1_PylRS-[pCNPR02] T3Te-T7Te DHFR

Depositor

Ahmed Badran

Article

Costello et al Nat Biotechnol. 2024 Sep 11. doi: 10.103

Insert

G1PylRS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

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