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IVT-donor_opt(R2Tg)-GFP
Plasmid#223246PurposeIn vitro transcription of the optimized R2Tg RNA donorDepositorInsertR2Tg donor_opt
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceAug. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-hSynI-V5-TurboID(N)-GPI
Plasmid#163696PurposeExpresses n-terminal half of TurboID on surface of neuronsDepositorInsertGPI anchored TurboID
UseAAVTagsExpressionMutationPromoterAvailable sinceMarch 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-ER alpha
Plasmid#28230PurposeMammalian expression of Estrogen Receptor alpha fused to EGFPDepositorInsertEstrogen Receptor alpha (ESR1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterAvailable sinceApril 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-jGCaMP8s
Plasmid#172389PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertGCaMP8s
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Laccase2 MCS Exon Vector
Plasmid#69893PurposeExpression plasmid for expressing circular RNAs of a desired sequence in mammalian cellsDepositorInsertLaccase2 (stw Fly)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-BP1-2 pLPC-Puro
Plasmid#19835DepositorInsertFragment of p53-Binding Protein 1 (TP53BP1 Human)
UseRetroviralTagsmCherryExpressionMutationFragment of human 53BP1 aa 1220 - 1711PromoterAvailable sinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBABE-neo-hTERT
Plasmid#1774PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
Circular-oc-EMCV-GFP
Plasmid#226261PurposeProduction of in vitro circularizable RNA via IVTDepositorInsertEGFP
UseIn vitro transcriptionTagsExpressionMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
N-Myc-53BP1 D1521A pLPC-Puro
Plasmid#19837DepositorInsertp53 Binding Protein 1 (TP53BP1 Human)
UseRetroviralTagsMycExpressionMutationchanged Asp1521 to AlaPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
KG#901
Plasmid#110936PurposeExpresses Emerald-RAB-3 in the cholinergic motor neuron DA9 for imaging synaptic vesicles in a single neuron in a living animalDepositorInsertsmig-13 promoter
Emerald-RAB-3
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CyclinF
Plasmid#32975DepositorInsertCYCLIN F (CCNF Human)
UseTagsFlag and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-MS2coatProtein
Plasmid#103832PurposeLive cell reader for MS2 loop RNA productionDepositorInsertmCherry-MS2 coat protein (cp )
UseTagsExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
114_UBI_iCre_crystGFP
Plasmid#171797PurposeTol2-plasmid ubiquitously expressing iCre (ubiquitin promoter) along with eGFP selection marker (Crystallin promoter -Lens/Eyes-)DepositorInsertoptimised iCre under the control of the Ubiquitin promoter
UseTagsExpressionMutationPromoterUbiquitinAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCEP-Puro-TetOn-L1RP-ORF1_StammerAAA_Halo-GFPai
Plasmid#202571PurposeExpresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the M91A, E92A, and L93A mutations and a GFP retrotransposition reporter (GFP-AI) in the 3' UTRDepositorInsertLINE-1
UseTagsGFP-AI retrotransposition reporter and HaloTag7 o…ExpressionMammalianMutationChanged ORF1p Methionine 91 to Alanine, ORF1p Glu…PromoterAvailable sinceApril 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 gfaABC1D-LifeAct-GFP
Plasmid#190199PurposeExpresses the small actin binding peptide, LifeAct, with a GFP reporterDepositorInsertLifeAct-eGFP
UseAAVTagsGFPExpressionBacterial and MammalianMutationPromotergfaABC1DAvailable sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-BioID2-BioID2-HA
Plasmid#176855PurposeExpresses the promiscuous small biotin ligase, BioID2, in neuronsDepositorInsertBioID2-BioID2-HA
UseAAVTagsHAExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pZac2.1 GfaABC1D rM3D mCherry SV40
Plasmid#92285PurposeAAV DREADD expressionDepositorInsertrM3D
UseAAVTagsmCherryExpressionMutationPromoterGfaABC1DAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-mTurquoise
Plasmid#172376PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertmTurquoise
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYES1L-URA
Plasmid#84301PurposeE.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceMay 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-nl.EGFP-SypGFP
Plasmid#172380PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertnuclear-localized EGFP + SypEGFP
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform1
Plasmid#70173PurposeMammalian expression of hsOPA1 isoform 1DepositorInserthsOPA1 isoform 1 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJuly 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES
Plasmid#69909PurposeExpresses a GFP circular RNA containing the sense EMCV IRES in mammalian cellsDepositorInsertZKSCAN1, GFP (ZKSCAN1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLS.405
Plasmid#187203PurposeExpresses wild-type retron Eco1 ncRNA + Eco1 RT, and Cas1 + Cas2DepositorInsertsRetron Eco1 ncRNA (wildtype)
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterT7/LacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform7
Plasmid#70179PurposeMammalian expression of hsOPA1 isoform 7DepositorInserthsOPA1 isoform 7 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
PZac2.1 hSyn1-Lck-GFP
Plasmid#177333PurposeExpresses GFP on the plasma membrane of neurons via an Lck localization sequenceDepositorInsertLck-GFP
UseAAVTagsGFPExpressionBacterialMutationPromoterhSyn1Available sinceApril 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform5
Plasmid#70177PurposeMammalian expression of hsOPA1 isoform 5DepositorInserthsOPA1 isoform 5 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform2
Plasmid#70174PurposeMammalian expression of hsOPA1 isoform 2DepositorInserthsOPA1 isoform 2 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform3
Plasmid#70175PurposeMammalian expression of hsOPA1 isoform 3DepositorInserthsOPA1 isoform 3 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform4
Plasmid#70176PurposeMammalian expression of hsOPA1 isoform 4DepositorInserthsOPA1 isoform 4 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
OPA1-isoform6
Plasmid#70178PurposeMammalian expression of hsOPA1 isoform 6DepositorInserthsOPA1 isoform 6 (OPA1 Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
MSCV-PIG-Cre
Plasmid#50935Purposeretroviral expression of Cre recombinaseDepositorInsertCre recombinase
UseCre/Lox and RetroviralTagsNLSExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBABE-SSeCKs
Plasmid#106908Purposefull-length murine SSeCKs/AKAP12/Gravin in retroviral vectorDepositorInsertSSeCKs (Akap12 Mouse)
UseRetroviralTagsExpressionMammalianMutationPromotergagAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXS-hs-3xHA-LIN-41
Plasmid#52717Purposeretroviral expression of human LIN-41 / TRIM71 with 3 N-terminal HA tagsDepositorInsertLIN-41 (TRIM71 Human)
UseRetroviralTags3xHAExpressionMammalianMutationPromoterAvailable sinceJune 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-rac2 guide
Plasmid#107600PurposeAn entry vector with U6a and U6c promoter driving rac2 guide RNAs expressionDepositorInsertrac2 guide RNA sequences
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
aav-SYN-tdNfsB(F124W)-mCherry (eNTR)
Plasmid#113760PurposeAAV-mediated expression of bacterial tdNfsB-mCherry (eNTR) under the SYN promoterDepositorInserttdNfsB(F124W)-mCherry
UseAAVTagsmCherryExpressionMammalianMutationF124WPromoterSynapsinAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1(7AW)RS
Plasmid#207516PurposetRNA synthetase/tRNA pair for the in vivo incorporation of 7-Aza-L-Tryptophan (7AW) into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available sinceOct. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
NMD2_lenti
Plasmid#216221PurposeNonsense mediated decay GFP P2A RFP reporter with two b-globin introns in lenti backboneDepositorInsertGFP P2A RFP_b-globin intron x2
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSBK.079
Plasmid#187218PurposeExpresses Eco1 RT and Cas1 + Cas2DepositorInsertsRetron Eco1 Reverse Transcriptase
Cas1 + Cas2
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterJ23115 and T7/lacAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
ptagRFP-MS2coatProtein
Plasmid#103831PurposeLive cell reader for MS2 loop RNA productionDepositorInsertMS2 coat protein (MCP) (cp )
UseTagstagRFPExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLEG(R1-R3) #BGV149
Plasmid#48956PurposeLentiviral Destination vector containing AttR1-AttR3 site. For use with three-way LR reaction with cDNA in entry vector and a marker in pBEG R2-L3 plasmids (module 2 and module 3 plasmids).DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pVG122
Plasmid#211765PurposeExpresses strongLOV under PGK1pr. StrongLOV is a variant of optogenetic transcription factor EL222 that is more sensitive than EL222 to blue light.DepositorInsertstrongLOV
UseFor single-copy integration (if cut with pmei)TagsSV40 NLS and VP16ExpressionYeastMutationcompared to EL222, strongLOV contains Glu84Asp mu…PromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-E2_Crimson
Plasmid#172377PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertE2_Crimson
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
KG#203
Plasmid#110880PurposeExpresses the C. elegans pde-4 (isoform d) cDNA pan-neuronallyDepositorInsertsrab-3 promoter
pde-4d (isoform d) cDNA
unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLEG(R1-R4) #BGV057
Plasmid#48957PurposeLentiviral Destination vector containing AttR1-AttR4 site. Use with 4-way LR reaction with cDNA in entry vector, marker in pBEG R2-L3 plasmids and shRNA in pBEG R3-L4 plasmid (module 2,3,and 4)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEG R3-miRNA(ccdB)-L4 #BGV228
Plasmid#48953PurposeTo create an AttR3-AttL4 flanked Entry vector encoding a miR30-embedded shRNA to knockdown targeted gene expression. (This corresponds to a module 4 plasmid).DepositorTypeEmpty backboneUseGateway cloning vectorTagsExpressionMutationPromoternaAvailable sinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTrex-Neo-tdTomato
Plasmid#47975PurposeTrasgenically expresses tandem tdtomato fluorescent protein in T. cruziDepositorInserttdTomato
UseTrypanasoma cruzi expressionTagsExpressionMutationPromoterT. cruzi rRNAAvailable sinceApril 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmKate2-MS2coatProtein
Plasmid#103833PurposeLive cell reader for MS2 loop RNA productionDepositorInsertmKate2-MS2 coat protein (cp )
UseTagsExpressionMammalianMutationMCP: nt 202-240 are missing (13 aa), C242G (A81G)…PromoterCMVAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
CVS-N2c-jGCaMP8m
Plasmid#172388PurposeProduction of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.DepositorInsertGCaMP8m
UseNeurotropic virusTagsExpressionMutationPromoterAvailable sinceSept. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMX-Flag-WIP
Plasmid#11772DepositorInsertWIP (WIPF1 Human)
UseRetroviralTagsFlagExpressionMammalianMutationPromoterAvailable sinceFeb. 15, 2007AvailabilityAcademic Institutions and Nonprofits only
