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Showing: 1751 - 1800 of 18210 results

  7. PB-tight-DDdCas9VP192-T2A-GFP-IRES-Neo

    Plasmid
    #102889
    Purpose
    PiggyBac transposon system construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Article
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Insert
    DDdCas9VP192-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102889-plasmid-map-sequence-id-198247

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  9. CAG-ABEmax-P2A-EGFP-ires-puro

    Plasmid
    #121170
    Purpose
    CAG promoter driven expression of ABEmax base editor, EGFP and Puromycin resistance.
    Depositor
    Timo Otonkoski
    Insert
    ABEmax-P2A-EGFP-ires-puro
    Use
    CRISPR
    Tags
    P2A-EGFP-ires-puro
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121170-plasmid-map-sequence-id-234985

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  14. pMXs-IRES-blast 3xFlag-SPNS1(E164K)

    Plasmid
    #198569
    Purpose
    SPNS1(E164K) codon-optimized orf with n-terminal 3xFlag in pMXs-IRES-Blast vector
    Depositor
    Monther Abu-Remaileh
    Article
    Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi
    Insert
    SPNS1(E164K) with n-terminal 3xFlag (SPNS1 Human)
    Use
    Retroviral
    Tags
    3xFlag
    Expression
    Mammalian
    Mutation
    changed glutamic acid E164 to lysine (E164K)
    Promoter
    Available since
    July 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    198569-plasmid-map-sequence-id-438870

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  19. pLenti NLuc398-hLGALS8 IRES hCALCOCO2-CLuc394

    Plasmid
    #128387
    Purpose
    Expresses a split firefly luciferase reporter based on the full length, human Gal8 and CALCOCO2, which interact following endosomal disruption
    Depositor
    Craig Duvall
    Article
    Kilchrist et al ACS Sens. 2020 Jun 23. doi: 10.1021/acss
    Inserts
    NLuc398-3xGGGGS-LGALS8 (LGALS8 Human, Synthetic)
    CALCOCO2-3xGGGGS-CLuc394 (CALCOCO2 Human)
    Use
    Lentiviral, Luciferase, and Synthetic Biology
    Tags
    CLuc394 and NLuc398
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and IRES
    Available since
    Jan. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    128387-plasmid-map-sequence-id-261539

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  23. mCherry-PTDSS1-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177435
    Purpose
    To express mCherry fused to PTDSS1, a protein that localized to mitochondria associated ER membranes (MAMs). Lentiviral vector used to make cell lines expressing this MAM landmark.
    Depositor
    David Andrews
    Insert
    Phosphatidylserine synthase-1, PTDSS1, LMHD, PSS1, PSSA (PTDSS1 Human)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177435-plasmid-map-sequence-id-348466

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  34. Direct-seq_lentiGuide-Puro-tRNA-Loop2-8A8G

    Plasmid
    #157986
    Purpose
    Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.
    Depositor
    Lijia Ma
    Article
    Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:
    Insert
    S. pyogenes sgRNA cassette (tRNA-Loop2-8A8G)
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hU6
    Available since
    May 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    157986-plasmid-map-sequence-id-332423

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  38. mCherry-ActA-IRES-puromycin-pLVx-EF1a

    Plasmid
    #177433
    Purpose
    To express mCherry fused to ActA mitochondria targeting sequence. Lentiviral vector used to make cell lines expressing this mitochondria landmark.
    Depositor
    David Andrews
    Insert
    ActA tail anchor (actA Listeria monocytogenes)
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2023
    Availability
    Academic Institutions and Nonprofits only
    177433-plasmid-map-sequence-id-348464

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  47. MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP

    Plasmid
    #75123
    Purpose
    Retroviral expression plasmid encoding a human BRD9 bromodomain-swap mutant containing the first bromodomain of BRD4 with a C terminal 3x FLAG-tag
    Depositor
    Christopher Vakoc
    Article
    Hohmann et al Nat Chem Biol. 2016 Jul 4. doi: 10.1038/
    Insert
    BRD9 (BRD9 Human)
    Use
    Retroviral
    Tags
    3x FLAG-tag
    Expression
    Mutation
    bromodomain replaced with the first bromodomain o…
    Promoter
    Available since
    May 18, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1751 - 1800 of 18210 results