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2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid
SB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance gene
The plasmid, pLXSN18E6E7, is used to produce amphotropic recombinant retrovirus providing efficient gene transfers and expression of the HPV18 E6 and E7 genes.
CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-CD19 from FMC63 in VL-VH order and 218 linker (scFv). GFP-Zeo for selection and monitoring.
lenti vector encoding the MS2-P65-HSF1 activator helper complex with a 2A Hygro resistance marker
NOTE: A version of this plasmid with improved titer is available: Addgene plasmid #89308 lentiMPH v2