-
Plasmid#201969PurposeORBIT integrating plasmid for C-terminal tagging with eGFP containing a 3C protease cleavage site.DepositorInsertattB-3C-eGFP-4xGly-TEV-Flag-6xHis
UseOrbit integrating plasmidTagsExpressionMutationPromoterAvailable sinceJuly 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
p5E-mfap4
Plasmid#70052Purpose5' Entry vector containing the zebrafish mfap4 promoter. Used with Gateway Recombination to generate transgenic constructs exhibiting macrophage-restricted expression of transgenes.DepositorInsertmfap4 promoter
UseGateway 5' elementTagsExpressionMutationPromoterAvailable sinceNov. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS2-gfp
Plasmid#109290PurposeExpresses high levels of gfp in mycobacteriaDepositorInsertGFP
UseTagsExpressionBacterialMutationWTPromoterKanamycinAvailable sinceJune 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pANTO5
Plasmid#131106PurposeTo generate an integrative plasmid carrying the TetR/Pip-OFF system, but not the integraseDepositorInserttetR gene by pFRA61 (Boldrin et al 2010)
UseTagsExpressionBacterialMutationPromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUM-Kan-MCS3
Plasmid#109287PurposeExpresses proteins in mycobacteriaDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationNew promoter/cloning sitePromoterAvailable sinceMay 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMINTNH3
Plasmid#110074PurposeIntegrative vector for expression in mycobacteria based on the Tet promoter. Contains an N-terminal His10 Tag followed by a 3C cleavage siteDepositorTypeEmpty backboneUseTagsHis10 Tag - 3C cleavage siteExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAGN33
Plasmid#131105PurposeAn integrative plasmid able to introduce a second copy of the pip gene in the chromosome of our conditional mutantsDepositorInsertP-furA102tetO-pip
UseTagsExpressionBacterialMutationthe furA102 promoter was mutated with the insertiā¦PromoterAvailable sinceDec. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSUM36-MCS2-myc
Plasmid#109379PurposeShuttle vector for high level expression in mycobacteria.DepositorInsertMCS2-Myc
UseTagsMycExpressionBacterialMutationPromoterKanamycinAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLV hUbC-Cas9-P2A-Puro_ST-sgRNA
Plasmid#188705PurposeLentivirus transfer plasmid encoding Cas9, puromycin resistance, and 4 sgRNAs targeting human safe targeting lociDepositorInsertST sgRNAs
UseLentiviralTagsExpressionMammalianMutationPromoterhUbCAvailable sinceDec. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEC3GH (Hyg)
Plasmid#110084PurposeReplicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.DepositorTypeEmpty backboneUseTags3C cleavage site - GFP - His10 TagExpressionBacterialMutationPromoterAvailable sinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only