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pSUI-SynP-CANTsg

Plasmid

#154343

Purpose

Expression of the sgRNA targeting to the E. coli can gene

Depositor

I-Son Ng

Article

Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

Insert

CANT sgRNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 22, 2020

Availability

Academic Institutions and Nonprofits only

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Antares-SEPLuc C1A4E

Plasmid

#141088

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES and weaker precursor of Nanoluc

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEP-C1A4E

Use

Tags

Expression

Mammalian

Mutation

Please see depositor comments

Promoter

CMV

Available since

June 26, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Mad3 [N129A/6R]

Plasmid

#140075

Purpose

Mammalian Expression Plasmid of anti-Mad3 (Human). Derived from hybridoma N129A/6.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Mad3 (Human) recombinant mouse monoclonal antibody (MXD3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

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Antares-SEPLuc IRESv24

Plasmid

#141087

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEPLuc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-Synaptotagmin-12 [N277/7R]

Plasmid

#114482

Purpose

Mammalian Expression Plasmid of anti-Synaptotagmin-12 (Mouse). Derived from hybridoma N277/7.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Synaptotagmin-12 (Mus musculus) recombinant mouse monoclonal antibody (Syt12 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

May 28, 2019

Availability

Academic Institutions and Nonprofits only

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pJET1.2_Nkx3-2_Anti-Sense

Plasmid

#124438

Purpose

Plasmid for anti-sense in situ probe in vitro transcription

Depositor

Yingguang Frank Chan

Article

Castro et al Elife. 2019 Jun 6;8:e42014. doi: 10.7554

Insert

NK3 homeobox 2 (Nkx3-2 Mouse)

Use

In situ probe

Tags

Expression

Mutation

Promoter

T7

Available since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223 SCAF4_CRISPR resistant

Plasmid

#122465

Purpose

pDONR for Gateway cloning

Depositor

Jesper Svejstrup

Article

Gregersen et al Cell. 2019 May 10. pii: S0092-8674(19)30

Insert

SCAF4 CRISPR resistant (SCAF4 Human)

Use

Gateway cloning

Tags

Expression

Mutation

Synonymous mutations conferring resistance to gRN…

Promoter

Available since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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PME-1_R369D mutant

Plasmid

#119294

Purpose

Mammalian overexpression of StrepII-tagged full length PPME1 with mutation in the PP2Ac binding motif

Depositor

Jukka Westermarck

Article

Pokharel et al Mol Cell Proteomics. 2015 Dec;14(12):327

Insert

PPME1 (PPME1 Human)

Use

Tags

Expression

Mammalian

Mutation

R369D

Promoter

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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Anti-Stonin-2 [N346/9R]

Plasmid

#114484

Purpose

Mammalian Expression Plasmid of anti-Stonin-2 (Human). Derived from hybridoma N346/9.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Stonin-2 (Homo sapiens) recombinant mouse monoclonal antibody (STON2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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Anti-Clcn4 [N222/6R]

Plasmid

#114474

Purpose

Mammalian Expression Plasmid of anti-Clcn4 (Human). Derived from hybridoma N222/6.

Depositor

James Trimmer

Article

Andrews et al Elife. 2019 Jan 22;8. pii: 43322. doi: 1

Insert

anti-Clcn4 (Homo sapiens) recombinant mouse monoclonal antibody (CLCN4 Mouse)

Use

Affinity Reagent/ Antibody

Tags

Expression

Mammalian

Mutation

Promoter

dual CMV

Available since

Sept. 20, 2018

Availability

Academic Institutions and Nonprofits only

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