Addgene

Showing: 7161 - 7180 of 52561 results

Results per page:

Sort results by:

Pdar

Plasmid

#24786

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

Pdar

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 4, 2011

Availability

Academic Institutions and Nonprofits only
pUHD Cdk2 WT HA

Plasmid

#27650

Depositor

Greg Enders

Article

Hu et al Mol Cell Biol. 2001 Apr . 21(8):2755-66.

Insert

Cdk2 (CDK2 Human)

Use

Tags

HA (YDVPDYA)

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2011

Availability

Academic Institutions and Nonprofits only
pNIR-Fb6m

Plasmid

#184680

Purpose

NIR-Fb to spike-Cov-19 RBD

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-Fb6m (S Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET-A2-77p-Venus

Plasmid

#64856

Purpose

Expresses aa 1594-1670 of A2 Domain of VWF protein, TEV cleavage site and Venus followed by 7X His tag

Depositor

Sriram Neelamegham

Article

Gogia et al PLoS One. 2015 May 18;10(5):e0126556. do

Insert

VWF A2 domain aa 1594-1670_TEV_Venus_7X His (VWF Human, Synthetic)

Use

Tags

7X His and Venus

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pFX-mKate2-Rab11

Plasmid

#174463

Purpose

Express ｍKate2 with recycling endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

mKate2 with recycling endosome-targeting sequence

Use

Tags

small GTPase (Rab11)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPK1E-1+t

Plasmid

#48147

Purpose

Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage K1E

Depositor

Dieter Jahn

Article

Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pBacPAK8-ElonginC

Plasmid

#29505

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Elongin C (ELOC Human)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pET15-His-∆NGlpG

Plasmid

#155131

Purpose

Expresses N-terminally His-tagged E. coli GlpG (residues 87-276) from pET15b

Depositor

Sinisa Urban

Article

Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

Insert

GlpG (glpG Escherichia coli)

Use

Tags

His

Expression

Bacterial

Mutation

insert codes for GlpG residues 87-276

Promoter

T7

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BB-NIC-II-HisN-TET12

Plasmid

#45496

Depositor

Roman Jerala

Article

Gradisar et al Nat Chem Biol. 2013 Apr 28. doi: 10.1038

Insert

TET12

Use

Synthetic Biology

Tags

6xHistidine tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDM8-GlyCAM-1/IgG

Plasmid

#17841

Depositor

Steven Rosen

Article

Bistrup et al J Cell Biol. 1999 May 17. 145(4):899-910

Insert

GlyCAM-1/IgG (Glycam1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2009

Availability

Academic Institutions and Nonprofits only
pmirGLO-Smad5-1719

Plasmid

#49382

Purpose

Insert is a fragment of the Smad5 3'-UTR that contains miR-17 and miR-19 binding sites. To be used for 3'UTR assay.

Depositor

Martine Roussel

Article

Murphy et al Cancer Res. 2013 Oct 21.

Insert

Smad5 (Smad5 Human, Mouse)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Human phosphoglycerate kinase (PGK) promoter

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pDEST_TO_EWSR1

Plasmid

#26376

Depositor

Thomas Tuschl

Article

Hoell et al Nat Struct Mol Biol. 2011 Nov 13. doi: 1

Insert

EWSR1 (EWSR1 Human)

Use

Tags

FLAG/HA

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 22, 2010

Availability

Academic Institutions and Nonprofits only
p3E-FRB(PLF)-HA no-pA

Plasmid

#82597

Purpose

3' entry vector containing HA epitope tag fused to C-terminus of FRB mutant (K2095P, T2098L, W2101F) without pA for rapamycin/rapalog induced dimerization

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

FRB-HA (no pA)

Use

Tags

Expression

Mutation

K2095P, T2098L, W2101F

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
GFP-mROCK1-delta5

Plasmid

#101293

Purpose

Mammalian expression of Rock1-GFP for the study of localisation signaling.

Depositor

Alpha Yap

Article

Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

Insert

Rock1 (Rock1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

375-727 aa

Promoter

CMV

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCellFree_KA2_ZIKV NS2A

Plasmid

#145363

Purpose

Cell-free/mammalian expression vector for Zika virus NS2A protein with N-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS2A

Use

Tags

His and mCherry

Expression

Bacterial

Mutation

Promoter

T7- CMV/CAG

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-Wnt2B2-V5

Plasmid

#35926

Depositor

Marian Waterman

Article

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt2B2 (WNT2B Human)

Use

Tags

V5

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
MDH1-PGK-FLAG-TAT-86-A

Plasmid

#35977

Depositor

Susana Valente

Article

Mediouni et al Curr HIV Res. 2015;13(1):64-79.

Insert

transactivating protein (tat Human Immunodeficiency Virus)

Use

RNAi and Retroviral

Tags

Flag

Expression

Mammalian

Mutation

From the "A" HIV clade

Promoter

Available since

April 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSETb-rsGreenF

Plasmid

#78181

Purpose

Expresses rsGreenF in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreenF

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pSB165 - pL1R3_pMAS::rLUC-I::tMAS

Plasmid

#123198

Purpose

binary plant vector for transient expression of a Renilla luciferase (rLUC, with intron)

Depositor

Carolyn Lee-Parsons, Erin Cram

Article

Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

Insert

pMAS::rLUC-I::tMAS

Use

Luciferase

Tags

Expression

Plant

Mutation

Promoter

Available since

May 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmCup_C

Plasmid

#146041

Purpose

Insect Expression of DmCup

Depositor

Elisa Izaurralde

Article

Tritschler et al Mol Cell Biol. 2008 Nov . 28(21):6695-70

Insert

DmCup (cup Fly)

Use

Tags

Expression

Insect

Mutation

P418S

Promoter

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge