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Nsp7-mCherry

Plasmid

#165134

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp7 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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Pol

Plasmid

#65520

Purpose

Expresses HBV polymerase in mammalian cells

Depositor

Wang-Shick Ryu

Article

Ryu et al Virology. 2008 Mar 30;373(1):112-23. Epu

Insert

HBV polymerase protein

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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Nsp8-EGFP

Plasmid

#165113

Purpose

mammalian expression and localization

Depositor

Bruno Antonny

Article

Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

Insert

Nsp8 (ORF1ab codon optimized SARS-COV-2)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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plentiCMV-sct-pp65-b2m-HLA-A0201

Plasmid

#196511

Purpose

Express single-chain peptide-MHC (pp65 CMV epitope on HLA-A0201)

Depositor

Howard Chang

Article

Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

Insert

single chain of a pp65 peptide, b2m and HLA A0201

Use

Lentiviral

Tags

NA

Expression

Mammalian

Mutation

Y84C

Promoter

CMV

Available since

March 1, 2023

Availability

Academic Institutions and Nonprofits only

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CDH5-AbR-GFP

Plasmid

#122970

Purpose

Lenti plasmid containing a CDH5 promoter driving GFP followed by T2A and an antibiotic resistance cassette (zeocin). Used for selection of endothelial cells.

Depositor

Budd A. Tucker

Inserts

CDH5p
CopGFP
Zeocin

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

NA

Promoter

Available since

May 15, 2019

Availability

Academic Institutions and Nonprofits only

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pLOX-TERT-iresTK

Plasmid

#12245

Purpose

2nd generation lentiviral vector for reversible immortalization of human primary cells

Depositor

Didier Trono

Article

Salmon et al Mol Ther. 2000 Oct . 2(4):404-14.

Insert

TERT, HSV1-TK (TERT Human)

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

LoxP in 3' LTR.

Promoter

Available since

Aug. 4, 2006

Availability

Academic Institutions and Nonprofits only

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pmScarlet_alphaTubulin_C1

Plasmid

#85045

Purpose

In vivo visualization of microtubules (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Alpha-Tubulin (TUBA1B Human)

Use

Tags

mScarlet

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

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NL4-3 mCherry Luciferase

Plasmid

#44965

Purpose

HIV dual reporter vector expressing mCh & Luc to simultaneously measure the # of cells containing reactivated latent provirus and the overall strength of viral transcriptional response in these cells.

Depositor

Warner Greene

Article

Lassen et al PLoS One. 2012;7(1):e30176. doi: 10.1371

Insert

mCherry, Luciferase, IRES

Use

Lentiviral

Tags

Luciferase, IRES and mCherry with D212G

Expression

Mutation

S34C mutation in Nef

Promoter

Available since

Oct. 30, 2015

Availability

Academic Institutions and Nonprofits only

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mEGFP-8xbiRhoBAST

Plasmid

#196351

Purpose

Expression of mEGFP mRNA with 8xbiRhoBAST in 3'UTR

Depositor

Murat Sunbul

Article

Buhler et al Nat Chem Biol. 2023 Apr;19(4):478-487. d

Insert

mEGFP-8xbiRhoBAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 15, 2023

Availability

Academic Institutions and Nonprofits only

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AAV2/9-CW3SL-mCherry-DrTrkB

Plasmid

#184002

Purpose

AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

Depositor

Vladislav Verkhusha, Dmitry Gerashchenko

Article

Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

Insert

eDrTrkB (NTRK2 Human)

Use

AAV

Tags

Ig kappa secretory signal, mCherry, Deinococcus r…

Expression

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis-MBP-BrCas12b-RFND

Plasmid

#195339

Purpose

Expresses Engineered BrCas12b in Bacteria

Depositor

Piyush Jain

Article

Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

Insert

BrCas12b

Use

CRISPR and Synthetic Biology

Tags

6xHis and 6xHis, MBP, TEV cleavage site

Expression

Bacterial

Mutation

R160E, F208W, N524V, D868V

Promoter

T7

Available since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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MAC-tag-N

Plasmid

#108078

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Use

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBL132

Plasmid

#104333

Purpose

Expresses taz from a LacI regulated promoter under inducible IPTG control.

Depositor

Jeffrey Tabor

Article

Landry et al Nat Commun. 2018 Apr 12;9(1):1433. doi:

Insert

Taz

Use

Tags

Expression

Bacterial

Mutation

Promoter

PtacI

Available since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-jAspSnFR3-mRuby3-NLS

Plasmid

#203494

Purpose

Nuclear directed jAspSnFR3-mRuby3 in lentiviral expression vector

Depositor

Lucas Sullivan

Article

Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

Insert

jAspSnFR3-mRuby3-NLS

Use

Lentiviral

Tags

Nuclear localization signal and mRuby3

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pH2B_mCherry_IRES_neo3

Plasmid

#21044

Depositor

Daniel Gerlich

Article

Steigemann et al Cell. 2009 Feb 6. 136(3):473-84.

Insert

pH2B

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pET His6 TEV LIC cloning vector (1B)

Plasmid

#29653

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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PRMT8-FLAG

Plasmid

#164699

Purpose

Expression of PRMT8 with a C-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Insert

PRMT8 (PRMT8 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PRB

Plasmid

#89130

Purpose

mammalian expression of full-length human progesterone receptor-B in pcDNA3, can be used to make stable cell line, select G418

Depositor

Elizabeth Wilson

Article

Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

Insert

human progesterone receptor-B (PGR Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-PKM2

Plasmid

#64698

Purpose

Expression of GFP tagged PKM2 in mammalian cells

Depositor

Axel Ullrich

Article

Stetak et al Cancer Res. 2007 Feb 15;67(4):1602-8.

Insert

PKM2 (PKM Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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EGFR-FR

Plasmid

#179263

Purpose

Human EGFR C-terminally labeled with FusionRed

Depositor

Jared Toettcher

Article

Farahani et al Cell Rep. 2021 Dec 28;37(13):110181. doi

Insert

EGFR (EGFR Human)

Use

Lentiviral

Tags

FusionRed

Expression

Mutation

Promoter

SFFV

Available since

Feb. 22, 2022

Availability

Academic Institutions and Nonprofits only

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