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Plasmid#124705PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLSDepositorInsertMCP_ADAR2 DD (E488Q)_NLS
UseAAVTagsNLSExpressionMammalianMutationE488Q hyperactive mutantPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-A382T
Plasmid#133756PurposeLentiviral expression of human TDP-43 A382T disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMutationChanged alanine 382 to threoninePromoterCMVAvailable sinceDec. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV.U6.shRNA-Sdc1-1376
Plasmid#195574PurposeExpresses shRNA to knockdown mouse Syndecan-1DepositorInsertshRNA, eGFP (Sdc1 Synthetic)
UseAAVTagsExpressionMammalianMutationPromoterU6Available sinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-H2B-mCerulean3-P2A-mKOFP2-CAAX
Plasmid#75154PurposeNuclear mCerulean2 (blue) fluorophore and a membrane targeted KOFP2 (orange) fluorophore separated by a self-cleaving 2A peptide for use with 5’ promoters.DepositorInsertH2B-mCerulean3-P2A-mKOFP2-CAAX
UseMiddle entry vector for multisite gateway three f…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTH41 [punc-17::YFP-BeCyclOp(A-2x)::SL2::mCherry]
Plasmid#168170PurposeExpression of YFP-BeCyclOp(A-2x) in cholinergic motor neurons of C. elegansDepositorInsertYFP-BeCyclOp(A-2x) (codon optimized for C. elegans)
UseTagsYFPExpressionWormMutationE497K and C566DPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR_L1-YFP-miniTurbo-NES-L2
Plasmid#127351Purposegateway entry vector for making N-terminal YFP-miniTurbo fusions with non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, NES, V5, and YFPExpressionMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_25H
Plasmid#91145PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt IIDepositorInsertEngineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPC1(P202A/F203A)-FLAG
Plasmid#164976PurposeLentiviral vector for NPC1 expression in mammalian cellsDepositorInsertNPC1 (NPC1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationP202A/ F203APromoterEF1aAvailable sinceMarch 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
MS2_adRNA-MCP_ADAR1 DD (E1008Q)_NLS
Plasmid#124626PurposeExpresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR1 DD(E1008Q)_NLSDepositorInsertMCP_ADAR1 DD (E1008Q)_NLS
UseAAVTagsNLSExpressionMammalianMutationE1008Q hyperactive mutantPromoterCMVAvailable sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-Cdc42BPB
Plasmid#50759Purposeexpression of EGFP tagged Cdc42BPBDepositorInsertCdc42BPB (CDC42BPB Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJan. 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDEST12.2 DYRK2 WT
Plasmid#20005DepositorInsertDYRK2 (DYRK2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceJan. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pXPR_003 sgSMARCA4 guide 1
Plasmid#193604PurposeSMARCA4 knockoutDepositorInsertsgSMARCA4 guide 1 (SMARCA4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_GNA14_G-alpha
Plasmid#109914PurposeProtein expression and purification of GNA14_G-alphaDepositorInsertGNA14_G-alpha (GNA14 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCFJ150-mCherry(dpiRNA)::ANI-1(AHPH)
Plasmid#107939Purposepie-1p::mCherry(dpiRNA)::ANI-1(AHPH)::pie-1 3'UTR construct used for MosSCI in nematode. mCherry is optimized by removing all piRNA targeting sites to allow germline expression. RhoA biosenser.DepositorInsertmCherry(dpiRNA)::ANI-1(AHPH)
UseTagsExpressionWormMutationPromoterpie-1Available sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHJ4: LexA-VHH-Caffeine
Plasmid#108242PurposeLexA DNA binding domain fusing with VHH antibody which can be dimerized by ligand caffeine. Upon ligand binding, LexA-VHH-Caffeine can repress down stream reporter gene sfGFP expression.DepositorInsertLexA DNA binding domain fusing with VHH-Caffeine antibody
UseSynthetic BiologyTagsExpressionMutationPromoterpLacO1Available sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MSCV-Luciferase-IRES-NrasG12D
Plasmid#60834PurposeMouse NrasG12D was amplified and mutated by PCR from IMAGE clone 6475312 (Invitrogen) and cloned downstream from the IRES; subsequently Luciferase was cloned upstream of the IRESDepositorInsertNrasG12D (Nras Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceNov. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
GFP-LiMETER-v1
Plasmid#113933PurposeExpress an optogenetic tether to label and interrogate ER-plasma membrane contact sites (MCS); visualized by GFPDepositorInsertLOV2 and Rit polybasic domain (PB)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMOD_C2616
Plasmid#91090PurposeModule C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol IIIDepositorInsertBsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
UseCRISPRTagsExpressionMutationPromoterAt7SLAvailable sinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry
Plasmid#67668PurposeZebrafish expression vector for ubiquitous expression of APEX2-GBPDepositorInsertAPEX2-GBP
UseZebrafish expression with tol2TagsExpressionMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_21C
Plasmid#91130PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt IIDepositorInsertEngineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only