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pAAV-MYL2-HA-TadA8e-Sauri N aa1-438-inteinN

Plasmid

#220128

Purpose

Expresses TadA8e and Sauri cas9N by MYL2 promoter, and add an HA tag fused to TadA

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSauri Cas9N, inteinN

Use

AAV

Tags

HA tag

Expression

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-Sauri C aa439-1061-U6-Camk2d sgRNA7

Plasmid

#220129

Purpose

Expresses Sauri cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSauri Cas9C

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-TadA8e-SpN aa2-713- inteinN-U6-Camk2d sgRNA7

Plasmid

#226915

Purpose

Expresses TadA8e and Sp cas9N by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, TadA, nSp Cas9N, inteinN

Use

AAV

Tags

Expression

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-MYL2-inteinC-SpC aa714-1368-U6-Camk2d sgRNA7

Plasmid

#226916

Purpose

Expresses Sp cas9C by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter

Depositor

Yuxuan Guo

Article

Liu et al Circ Res. 2025 Jan 16. doi: 10.1161/CIRC

Insert

MYL2, inteinC, nSp Cas9C

Use

AAV

Tags

Expression

Mutation

Promoter

MYL2

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Plasmid

#200793

Purpose

Mammalian expression of CLIPf-KIF5A(1-914; Δ505-610)-SNAPf-HisTag-FLAG

Depositor

Alison Twelvetrees

Article

Smith et al bioRxiv 2024.12.20.629623

Insert

KIF5A (Kif5a Mouse)

Use

Tags

CLIPf and SNAPf, HisTag, FLAG

Expression

Mammalian

Mutation

1-914; Δ505-610

Promoter

CMV & T7

Available since

Dec. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pPD1178-LPDV promoterless PuroR-P2A-miRFP720

Plasmid

#201537

Purpose

Landing pad destination vector for mMoClo golden gate-based assembly of landing pad integration vectors; contains BxB1 attB site, promoterless puromycin resistance gene and miRFP720 gene

Depositor

Joshua Leonard

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

Promoterless PuroR-P2A-miRFP720

Use

Tags

Expression

Mammalian

Mutation

Promoter

None

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-CMV-GFP-P2A-K20-P2A-mCherry

Plasmid

#185618

Purpose

Ribosomal stalling reporter: positive control

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

GFP-K20(AAA)-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-CMV-GFP-P2A-K0-P2A-mCherry

Plasmid

#185619

Purpose

Ribosomal stalling reporter: empty

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

GFP-empty-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-CMV-GFP-P2A-I15-P2A-mCherry

Plasmid

#185620

Purpose

Ribosomal stalling reporter: 15x isoleucine

Depositor

Sabine Fuchs

Article

Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

Insert

GFP-I15-mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-2_ST-10 kb

Plasmid

#218314

Purpose

Donor plasmid of Tc1-2_ST Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-MAG-10 kb

Plasmid

#218315

Purpose

Donor plasmid of MAG Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-8B_DR-10 kb

Plasmid

#218318

Purpose

Donor plasmid of Tc1-8B_DR Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-Tc1-1_PM-10 kb

Plasmid

#218319

Purpose

Donor plasmid of Tc1-1_PM Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-piggyBac-1_AMi-10 kb

Plasmid

#218320

Purpose

Donor plasmid of piggyBac-1_AMi Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-SB100X-10 kb

Plasmid

#218313

Purpose

Donor plasmid of SB100X Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQLL-Tc1-1_Xt-10 kb

Plasmid

#218316

Purpose

Donor plasmid of Tc1-1_Xt Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pQLL-Tc1-3_FR-10 kb

Plasmid

#218317

Purpose

Donor plasmid of Tc1-3_FR Transposon with 10 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

10 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMV-SB100X-5 kb

Plasmid

#218303

Purpose

Donor plasmid of SB100X Transposon with 5 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

5 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMV-Tc1-2_ST-5 kb

Plasmid

#218304

Purpose

Donor plasmid of Tc1-2_ST Transposon with 5 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

5 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pQLL-Tc1-1_Xt-5 kb

Plasmid

#218306

Purpose

Donor plasmid of Tc1-1_Xt Transposon with 5 kb cargo gene

Depositor

Haoyi Wang

Article

Zhang et al Cell. 2024 Jul 11;187(14):3741-3760.e30.

Insert

5 kb cargo gene

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge