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pAAV-TRE-mAmetrine1.1-WPRE

Plasmid

#193340

Purpose

AAV-mediated expression of mAmetrine1.1 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mAmetrine1.1

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-hyPE2

Plasmid

#177173

Purpose

Expresses hyPE2 in mammalian cells

Depositor

Hyongbum Kim

Article

Song et al Nat Commun. 2021 Sep 23;12(1):5617. doi:

Insert

hyperPE2

Use

CRISPR

Tags

SV40 NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-mNeonGreen-WPRE

Plasmid

#193341

Purpose

AAV-mediated expression of mNeonGreen under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Leiwe et al Nat Commun. 2024 Jun 25;15(1):5279. doi:

Insert

mNeonGreen

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

TRE

Available since

Aug. 22, 2023

Availability

Academic Institutions and Nonprofits only

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HA-tag

Plasmid

#55182

Purpose

HA-tag

Depositor

Thorsten Mascher

Article

Radeck et al J Biol Eng. 2013 Dec 2;7(1):29. doi: 10.

Insert

HA tag

Use

Synthetic Biology; Bacillus biobrick box

Tags

Expression

Mutation

Promoter

Available since

July 7, 2014

Availability

Academic Institutions and Nonprofits only

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Lenti:FlipGFP-T2A-mCherry

Plasmid

#223198

Purpose

Lentiviral vector encoding FlipGFP-T2A-mCherry (w/ caspase-3 cleave site in FlipGFP for restoring GFP conformation)

Depositor

Yeh-Hsing Lao

Article

Chi et al Lab Chip. 2024 Jan 30;24(3):396-407. doi

Insert

FlipGFP-T2A-mCherry

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Sept. 12, 2024

Availability

Academic Institutions and Nonprofits only

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TagRFP-T_A1aY1

Plasmid

#158751

Purpose

Lentiviral vector for expression of TagRFP-T_A1aY1 in mammalian cells for detection of tyrosinated microtubules.

Depositor

Minhajuddin Sirajuddin

Article

Kesarwani et al J Cell Biol. 2020 Oct 5;219(10). pii: 15

Insert

TagRFP-T_A1aY1

Use

Lentiviral

Tags

TagRFP-T

Expression

Mammalian

Mutation

Promoter

CAG (CMV enhancer and chicken beta actin promoter)

Available since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pCI HA NEDD4L

Plasmid

#27000

Depositor

Joan Massague

Article

Gao et al Mol Cell. 2009 Nov 13. 36(3):457-68.

Insert

NEDD4L (NEDD4L Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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p5E-EF1a/b-actin

Plasmid

#82583

Purpose

5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

Depositor

Kryn Stankunas

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter

Use

Tags

Expression

Mutation

Promoter

None

Available since

Dec. 21, 2016

Availability

Academic Institutions and Nonprofits only

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peGFP C3-SPG20

Plasmid

#218578

Purpose

Expresses GFP-tagged human SPG20 in mammalian cells

Depositor

Hemmo Meyer

Article

Gahlot et al Mol Cell. 2024 Mar 14:S1097-2765(24)0017

Insert

SPG20 (SPART Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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#1.4 post-G-SynapShot-mScarlet

Plasmid

#214716

Purpose

Expresses green #1.4 post-SynapShot and mScarlet

Depositor

Won Do Heo

Article

Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

Insert

#1.4 post-G-SynapShot

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

March 21, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-mScarlet-Gephyrin.FingR-IL2RGTC

Plasmid

#125695

Purpose

Global labeling of inhibitory synapses (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

mScarlet-Gephyrin.FingR-IL2RGTC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHLP710

Plasmid

#203740

Purpose

Integration of OsTIR1-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Expression

Mutation

Promoter

ACT1 (C. albicans)

Available since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-EF1A-Gephyrin.FingR-eGFP-CCR5TC

Plasmid

#125692

Purpose

Global labeling of inhibitory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

Gephyrin.FingR-eGFP-CCR5TC

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

EF1A

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-STIM1

Plasmid

#228952

Purpose

Retroviral expression of mCherry-STIM1

Depositor

David Pellman

Article

Zhao et al Dev Cell. 2023 May 22;58(10):847-865.e10

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA Flag-Smad4M

Plasmid

#14959

Depositor

Joan Massague

Article

Kang et al Proc Natl Acad Sci U S A. 2005 Sep 27. 1

Insert

Smad4 (SMAD4 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 17, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3.tgoGAP1

Plasmid

#78737

Purpose

Expression of Ca2+ sensor GAP1 in the trans Golgi apparatus

Depositor

Teresa Alonso

Article

Rodriguez-Garcia et al Proc Natl Acad Sci U S A. 2014 Feb 18;11

Insert

tgoGAP1

Use

Tags

N terminal 81 amino acids of human galactosyltran…

Expression

Mammalian

Mutation

D117A/D119A/D163A in aequorin moiety

Promoter

CMV

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pET-16b-10His-TEV-MCES_VACCA_D1_D12

Plasmid

#203671

Purpose

The D1 and D12 subunits of the vaccinia capping enzyme were cloned in tandem into the vector pET-16b (Novagen). An N-terminal His10 tag was added to the D1 subunit and the original factor Xa cleavage

Depositor

Stephen Cusack

Article

Kyrieleis et al Structure. 2014 Mar 4;22(3):452-65. doi:

Insert

MCES_VACCA_D1_D12

Use

Tags

10xHis

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+N-eGFP_SAMD9L

Plasmid

#206173

Purpose

mammalian expression of WT SAMD9L

Depositor

Christopher Goodnow

Article

Russell et al Proc Natl Acad Sci U S A. 2021 Aug 24;11

Insert

SAMD9L (SAMD9L Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3-6XHis-human SUMO 1

Plasmid

#133770

Purpose

express 6Xhis- SUMO 1 protein

Depositor

Martine Roussel

Article

Tago et al Proc Natl Acad Sci U S A. 2005 May 24. 1

Insert

SUMO 1 (SUMO1 Human)

Use

Tags

6XHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pFX-EGFP-EEA1

Plasmid

#174451

Purpose

Express EGFP with early endosome-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Article

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

EGFP with early endosome-targeting sequence

Use

Tags

early endosomal antigen 1 (EEA1)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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