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pCR8 JARID2

Plasmid

#114443

Purpose

Entry vector for gateway cloning containing human JARID2 coding sequence

Depositor

Adrian Bracken

Article

Conway et al Mol Cell. 2018 May 3;70(3):408-421.e8. d

Insert

JARID2 (JARID2 Human)

Use

Gateway entry cloning

Tags

Expression

Mutation

Promoter

None

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pDmDERusk

Plasmid

#213702

Purpose

Vector for red-light-induced bacterial gene expression

Depositor

Andreas Moeglich

Article

Meier et al Nat Commun. 2024 Jun 10;15(1):4876. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 30, 2024

Availability

Academic Institutions and Nonprofits only

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paGFP-Omp25

Plasmid

#69598

Purpose

expresses GFP-labeled mitochondrial outer membrane protein 25

Depositor

David Sabatini

Article

Katajisto et al Science. 2015 Apr 17;348(6232):340-3. do

Insert

paGFP-Omp25 (Synj2bp Rat)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

photoactivatable GFP fused to rat Omp25 C-terminus

Promoter

CMV

Available since

Dec. 18, 2015

Availability

Academic Institutions and Nonprofits only

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Proinsulin-NanoLuc in pLX304

Plasmid

#62057

Purpose

Luminescent reporter of insulin secretion

Depositor

David Altshuler

Article

Burns et al Cell Metab. 2015 Jan 6;21(1):126-37. doi

Insert

Proinsulin-NanoLuc (Ins2 Mouse, Synthetic)

Use

Lentiviral and Luciferase

Tags

Expression

Mammalian

Mutation

Inserted NanoLuc into C-peptide of mouse Ins2 cod…

Promoter

CMV

Available since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV.GfaABC1D.PI.Cre.SV40

Plasmid

#105603

Purpose

AAV expression of Cre from GfaABC1D promoter

Depositor

Baljit Khakh

Insert

Cre

Use

AAV and Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

GfaABC1D

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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Human J-chain

Plasmid

#158215

Purpose

Express the human J-chain

Depositor

Junyu Xiao

Article

Li et al Science. 2020 Feb 28;367(6481):1014-1017

Insert

JCHAIN (JCHAIN Human)

Use

Tags

8×his

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

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TroponinT-GCaMP5-Zeo

Plasmid

#46027

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

GCaMP5-2A-Zeo

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

TNNT2

Available since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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pVP91A-pcaHG

Plasmid

#113766

Purpose

Expresses the alpha (pcaG) and the beta (pcaH) subunits of Protocatechuate 3,4-Dioxygenase from Pseudomonas putida.

Depositor

John Lipscomb

Article

Knoot et al Proc Natl Acad Sci U S A. 2015 Jan 13;11

Inserts

β subunit of Protocatechuate 3,4- Dioxygenase
α subunit of Protocatechuate 3,4- Dioxygenase

Use

Tags

Met-6xHis

Expression

Bacterial

Mutation

Promoter

T5 promoter

Available since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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GST-TEV-FUS

Plasmid

#29629

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

GST and TEV

Expression

Bacterial

Mutation

Promoter

Available since

July 18, 2011

Availability

Academic Institutions and Nonprofits only

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pCVD442

Plasmid

#11074

Depositor

James Kaper, Michael Donnenberg

Article

Donnenberg et al Infect Immun. 1991 Dec . 59(12):4310-7.

Insert

sacB (sacB Bacillus subtilis)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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hyA3A-BE4max

Plasmid

#157943

Purpose

Mammalian expression of hyA3A-BE4max

Depositor

Dali Li

Article

Zhang et al Nat Cell Biol. 2020 Jun;22(6):740-750. d

Insert

hyA3A-BE4max

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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Lenti-CamKII-PSD95.FingR-eGFP-CCR5TC

Plasmid

#126218

Purpose

Global labeling of excitatory synapses (green fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95.FingR-eGFP-CCR5TC

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CamKIIa

Available since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-GST [N100/13R ]

Plasmid

#188214

Purpose

Mammalian Expression Plasmid of anti-GST (Schistosoma japonicum). Derived from hybridoma N100/13

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-GST (Schistosoma japonicum) recombinant mouse monoclonal antibody

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pNM3823

Plasmid

#154123

Purpose

mec-4 promoter driving tet OFF tetR-L-QFAD in an RCME vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2020 Jun 8. pii: genetics.120.

Insert

mec-4p::tetR-L-QFAD::act-4 '3

Use

Rmce

Tags

Expression

Worm

Mutation

Promoter

not applicable

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223_ATG16L1_WT

Plasmid

#82255

Purpose

Gateway Donor vector containing ATG16L1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

ATG16L1 (ATG16L1 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

WT

Promoter

None

Available since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-GFP-progerin

Plasmid

#17663

Depositor

Tom Misteli

Article

Scaffidi et al Nat Cell Biol. 2008 Mar 2. ():.

Insert

GFP-D50 lamin A (LMNA Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

Delta 50

Promoter

Available since

March 27, 2008

Availability

Academic Institutions and Nonprofits only

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pCSC-ISL1-T2A-LHX3

Plasmid

#90215

Purpose

To convert human skin fibroblasts into induced motor neurons (hiMN) in combination with NGN2, Sox11, FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Cell Rep. 2016 Jan 5;14(1):115-28. doi:

Insert

ISL1-T2A-LHX3 (ISL1, LHX3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGFP

Plasmid

#32748

Depositor

Jeanne Hardy

Article

Nicholls et al J Biol Chem. 2011 Jul 15;286(28):24977-8

Insert

GFP

Use

Tags

DEVDFQGPCNDSSDPLVVAASIIGILHLILWILDRL

Expression

Mammalian

Mutation

S65T

Promoter

CMV

Available since

March 5, 2012

Availability

Academic Institutions and Nonprofits only

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pCEP4 Smad4

Plasmid

#16483

Depositor

Bert Vogelstein

Article

Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

Insert

Smad4 (SMAD4 Human)

Use

Tags

Double HA

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
Dead-Laconic/pcDNA3.1(-)

Plasmid

#118627

Purpose

To express in mammalian cells a lactate FRET sensor negative control.

Depositor

Luis Felipe Barros

Article

Baeza-Lehnert et al Methods Mol Biol. 2020;2184:19-30. doi:

Insert

Dead-Laconic

Use

Tags

Expression

Mammalian

Mutation

Change Histidine 151 to Aspartic Acid of Escheric…

Promoter

CMV Promoter

Available since

Dec. 19, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-H2B-mScarlet-I

Plasmid

#182297

Purpose

Localization of mScarlet-I fluorescent protein to histone 2B

Depositor

Severine Degrelle, Thierry Fournier

Article

Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

Insert

mScarlet-I

Use

Tags

mScarlet-I

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-double floxed-mCherry-WPRE-HGHpA

Plasmid

#20299

Purpose

Cre-activated AAV expression of mCherry

Depositor

Karl Deisseroth

Insert

mCherry

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESRRA_Hormone-recep

Plasmid

#109908

Purpose

Protein expression and purification of ESRRA_Hormone-recep

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESRRA_Hormone-recep (ESRRA Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pPR219.05

Plasmid

#78554

Purpose

Plasmid expressing UirR, response regulator and PcsiR1-regulated GFP output

Depositor

Jeffrey Tabor

Article

Ramakrishnan et al ACS Synth Biol. 2016 Apr 27.

Insert

uirR

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

J23115

Available since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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VSVG-mCherry-RUSH

Plasmid

#203647

Purpose

RUSH cargo: Str-KDEL_IRES_SBP-mCherry-VSVG

Depositor

Juan Bonifacino

Article

Chen et al J Cell Biol. 2017 Dec 4;216(12):4141-415

Insert

VSVG

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pTOX11

Plasmid

#127456

Purpose

Encodes MqsR toxin. Gent-R

Depositor

Matthew Waldor

Article

Lazarus et al Appl Environ Microbiol. 2019 Jun 14. pii

Insert

mqsR toxin

Use

Tags

Expression

Bacterial

Mutation

Promoter

pRha

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-FLAG-Ku70

Plasmid

#46957

Purpose

Plasmid for cloning and epression of GFP-FLAG tagged human Ku70 (N-terminal tag). Confers resistance to G418.

Depositor

Steve Jackson

Article

Britton et al J Cell Biol. 2013 Jul 29.

Insert

Ku70 (XRCC6 Human)

Use

Tags

GFP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pBWB419

Plasmid

#140637

Purpose

RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/CIP2A(1-905) WT V5 His

Plasmid

#119287

Purpose

CIP2A WT V5 overexpression

Depositor

Jukka Westermarck

Article

Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

Insert

KIAA1524 (CIP2A Human)

Use

Tags

V5 His

Expression

Mammalian

Mutation

Promoter

T7

Available since

Jan. 14, 2019

Availability

Academic Institutions and Nonprofits only

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COVID-SARS2 NSP13

Plasmid

#159614

Purpose

Bacterial expression plasmid for COVID-SARS2 NSP13 helicase

Depositor

Opher Gileadi

Article

Newman et al Nat Commun. 2021 Aug 11;12(1):4848. doi:

Insert

Covid-SARS2 Nsp13

Use

Tags

His6-Zb-TEV

Expression

Bacterial

Mutation

Codon-optimized for E. coli expression

Promoter

T7 - LacO

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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