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plentiCRISPR-v2 Puro FLVCR1_sg5
Plasmid#218522PurposesgRNA targeting human FLVCR1DepositorInsertFLVCR1 (FLVCR1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v2 Puro CHKA_sg2
Plasmid#218523PurposesgRNA targeting human CHKADepositorInsertCHKA (CHKA Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
plentiCRISPR-v1 GFP FLVCR1_sg5
Plasmid#218521PurposesgRNA targeting human FLVCR1DepositorInsertFLVCR1 (FLVCR1 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-moxGFP-LMNB1
Plasmid#207772PurposeDonor template for Blast-2A-moxGFP insertion into the N-terminus of the LMNB1 locus for nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770DepositorArticleInsertLMNB1 Homology Arms flanking a Blast-moxGFP Cassette (LMNB1 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pKLV2-U6gRNA5(ZNF649_5-1)-PGKpuroBFP-W
Plasmid#212000PurposeExpress gRNA against ZNF649 with puro and BFPDepositorInsertsgRNA targeting ZNF649 (ZNF649 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZNF649_5-5)-PGKpuroBFP-W
Plasmid#212001PurposeExpress gRNA against ZNF649 with puro and BFPDepositorInsertsgRNA targeting ZNF649 (ZNF649 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(ZNF90_5-3)-PGKpuroBFP-W
Plasmid#212002PurposeExpress gRNA against ZNF90 with puro and BFPDepositorInsertsgRNA targeting ZNF90 (ZNF90 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NANOG_g1)-PGKpuroBFP-W
Plasmid#211974PurposeExpress gRNA against NANOG with puro and BFPDepositorInsertsgRNA targeting NANOG (NANOG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(NANOG_g2)-PGKpuroBFP-W
Plasmid#211975PurposeExpress gRNA against NANOG with puro and BFPDepositorInsertsgRNA targeting NANOG (NANOG Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceJan. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Puro-mNeon-ACTB
Plasmid#207752PurposeDonor template for Puro-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Puro-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Blast-mNeon-ACTB
Plasmid#207751PurposeDonor template for Blast-2A-mNeon insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a Blast-mNeon Cassette (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9-A695Q
Plasmid#207368PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A695Q
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, R661A, K848A, Q926A, K1003A, R1060A, amino…PromoterCBhAvailable sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9-A926Q
Plasmid#207370PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A926Q
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, R661A, Q695A, K848A, K1003A, R1060A, amino…PromoterCBhAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-evoSpCas9-E526K
Plasmid#207365PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-evoSpCas9-E526K
UseCRISPRTags3xFLAGExpressionMammalianMutationM495V, Y515N, R661Q, amino acids 1005-1013 replac…PromoterCBhAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-evoSpCas9-V495M
Plasmid#207363PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-evoSpCas9-V495M
UseCRISPRTags3xFLAGExpressionMammalianMutationY515N, K526E, R661Q, amino acids 1005-1013 replac…PromoterCBhAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HiFi SpCas9
Plasmid#207362PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HiFi SpCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationR691A, amino acids 1005-1013 replaced with two gl…PromoterCBhAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-Sniper SpCas9
Plasmid#207361PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-Sniper SpCas9
UseCRISPRTags3xFLAGExpressionMammalianMutationF539S, M763I, K890N, amino acids 1005-1013 replac…PromoterCBhAvailable sinceOct. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
B-HeFSpCas9-A1060R
Plasmid#207372PurposeExpression plasmid for human codon-optimized increased-fidelity (i.e. high-fidelity) SpCas9 variantDepositorInsertB-HeFSpCas9-A1060R
UseCRISPRTags3xFLAGExpressionMammalianMutationN497A, R661A, Q695A, K848A, Q926A, K1003A, amino …PromoterCBhAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only
