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pRK5 TGF beta type I receptor (T202D) Flag

Plasmid

#14833

Depositor

Rik Derynck

Article

Feng et al J Biol Chem. 1996 May 31. 271(22):13123-

Insert

TGF beta type I receptor (Tgfbr1 Rat)

Use

Tags

flag

Expression

Mammalian

Mutation

Threonine 202 mutated to aspartate. Constitutivel…

Promoter

Available since

May 15, 2007

Availability

Academic Institutions and Nonprofits only

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pGIPz-PD-L1 WT

Plasmid

#121486

Purpose

2nd generation lentiviral vector for over expressing human PD-L1 WT with PD-L1 shRNA

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

PD-L1

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pPBbsr2-4031NES

Plasmid

#105241

Purpose

A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

Depositor

Michiyuki Matsuda

Article

Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

Insert

AMPK-EV

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAGGS

Available since

Feb. 22, 2018

Availability

Academic Institutions and Nonprofits only

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TFRC-His-bio

Plasmid

#52338

Purpose

Expresses full-length Transferrin receptor protein 1 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

TFRC (TFRC Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pMOD_B2312

Plasmid

#91070

Purpose

Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers

Use

CRISPR

Tags

Expression

Mutation

Promoter

PvUbi1

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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LVDP-CArG-RE-GPR

Plasmid

#89762

Purpose

Quantitative measurement of CArG response element activity.

Depositor

Stelios Andreadis

Article

Mistriotis et al Stem Cells. 2017 Jan;35(1):207-221. doi:

Insert

6X CArG-RE

Use

Lentiviral

Tags

CMV-ZsGreen

Expression

Mammalian

Mutation

Promoter

Available since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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CMV-FlicR1

Plasmid

#74142

Purpose

Mammalian expression of FlicR1: A bright red fluorescent genetically encoded voltage indicator

Depositor

Robert Campbell

Article

Abdelfattah et al J Neurosci. 2016 Feb 24;36(8):2458-72. d

Insert

FlicR1

Use

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pMOD_B2103

Plasmid

#91061

Purpose

Module B, Promoter: CmYLCV, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Tags

Expression

Mutation

Promoter

CmYLCV

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pSL0812 (pCRISPR(BbsI)_Vch)

Plasmid

#200898

Purpose

Expresses V. cholerae CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Article

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

VchCRISPR (entry)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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miniSOG-H2B-C-10

Plasmid

#57769

Purpose

Localization: Nucleus/Histones, Excitation: 448 / 473, Emission: 500 / 528

Depositor

Michael Davidson

Article

Shu et al PLoS Biol. 2011 Apr;9(4):e1001041. doi:

Insert

H2B (H2BC11 Human)

Use

Tags

miniSOG

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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SP-GFP-CD274-NU

Plasmid

#121139

Purpose

Mammalian expression of GFP tagged CD274

Depositor

Christine Mayr

Article

Ma et al Cell. 2018 Oct 30. pii: S0092-8674(18)31

Inserts

CD274 (CD274 Human)
CD274 (CD274 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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ATOH1_pUC18

Plasmid

#191250

Purpose

Encodes the sequence for the ATOH1 gene (human)

Depositor

Chen Davidovich

Article

Zhang et al Nat Commun. 2021 Jul 28;12(1):4592. doi:

Insert

ATOH1 (ATOH1 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

lac promoter

Available since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pMSCV-mCherry-Vav1

Plasmid

#50044

Purpose

Expression of mCherry-Vav1 in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Vav1 (Vav1 Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pMOD_B2112

Plasmid

#91064

Purpose

Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers , Terminator: 35S

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

SapI ccdb cassette for cloning multiple gRNA spacers with Csy4 spacers

Use

CRISPR

Tags

Expression

Mutation

Promoter

PvUbi1

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMSCV-mCherry-Syk

Plasmid

#50045

Purpose

Expression of mCherry-Syk in mammalian cells

Depositor

Hidde Ploegh

Article

Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

Insert

Spleen tyrosine kinase (Syk) (Syk Mouse)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 13, 2014

Availability

Academic Institutions and Nonprofits only

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pET30a-SARS-CoV2-NP

Plasmid

#165090

Purpose

protein expression in E. coli

Depositor

Rick Tarleton

Article

Shen et al medRxiv [Preprint]. 2021 Jan 27:2021.01.

Insert

Severe acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal (N SARS-CoV2)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 10, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-RAB-EKARev-NLS

Plasmid

#118476

Purpose

ddRFP-based single-color ERK biosensor targeted to the nucleus.

Depositor

Jin Zhang

Article

Mehta et al Nat Cell Biol. 2018 Sep 24. pii: 10.1038

Insert

RAB-EKARev-NLS

Use

Tags

6xHIS, Nuclear localization signal (NLS), T7 tag …

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NES

Plasmid

#124707

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NES

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NES

Use

AAV

Tags

NES

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1 3xFlag-CREB

Plasmid

#82721

Purpose

Expresses human CREB

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

CREB (CREB1 Human)

Use

Tags

3XFLAG and GST

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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Ad-EphA3 WT

Plasmid

#102725

Purpose

Expression of WT EphA3

Depositor

Jin Chen

Article

Frieden et al Dev Dyn. 2010 Dec;239(12):3226-34. doi:

Insert

EphA3 (Epha3 Mouse)

Use

Adenoviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 4, 2017

Availability

Academic Institutions and Nonprofits only

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