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cMyc-tag
Plasmid#55183PurposecMyc tagDepositorInsertcMyc tag
UseSynthetic Biology; Bacillus biobrick boxTagsExpressionMutationPromoterAvailable sinceJuly 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSLC-242
Plasmid#73189PurposeTemplate plasmid which encodes chloramphenicol resistance gene for positive selection and a toxin gene (relE) under the control of rhamnose induceable promoter (PrhaB) for negative selection.DepositorInsertchloramphenicol (cat Although Ampicillin gene is present, it was inactivated while cloning.)
UseTagsExpressionBacterialMutationPromoterchloramphenicol promoterAvailable sinceJuly 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-CRY2-mCherry
Plasmid#178525PurposeExpresses CRY2-mCherry in mammalian cellsDepositorInsertCRY2-mCherry
UseTagsExpressionMammalianMutationPromoterCAGAvailable sinceDec. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSB161 - pL2_pSB90_2x35S::GUS::tMAS
Plasmid#123197Purposebinary plant vector for transient expression of GUS (with introns)DepositorInsert2x35S::GUS::tMAS
UseTagsExpressionPlantMutationPromoterAvailable sinceMay 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
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pLenti-STAT3-linker-TagGFP2
Plasmid#121427PurposeWT STAT3 inserted using Infusion Cloning into MluI and EcoRI digested pLenti-Origene-Nrf21.DepositorInsertSTAT3-linker-TagGFP2
UseTagsTagGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCSN061
Plasmid#101725PurposeSingle copy yeast plasmid expressing Cas9 from Streptococcus pyogenes (SpCas9), codon optimized for expression in Saccharomyces cerevisiae.DepositorInsertsS. pyogenes Cas9 codon optimized for expression in S. cerevisiae. (NEWENTRY )
KanMX marker expression cassette.
UseCRISPRTagsSV40 NLSExpressionYeastMutationPromoterHeterologous TEF1 promoter from A. gossypii. and …Available sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA RBD-GFP11
Plasmid#133069PurposeExpresses RBD domain with tripartite split GFP11 (M4)DepositorInsertRBD (Rhotekin-Rho Binding Domain)
UseTagsGFP11 (tripartite split-GFP)ExpressionMammalianMutationPromoterCMVAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mSmo-WT-V5
Plasmid#126415PurposeLocalization, Expression of Smoothened WT in mammalian cell lines.DepositorInsertm-Smoothened (Smo Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFX-GalT-EGFP
Plasmid#174471PurposeExpress EGFP with golgi apparatus-targeting sequence in mammalian cellsDepositorInsertEGFP with golgi apparatus-targeting sequence
UseTagsβ1,4-galactose transferaseExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2VL-IRES-dOrai
Plasmid#67629PurposeMammalian expression of dmBACCS2VL, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, and dOraiDepositorInsertdmBACCS2VL-IRES-dOrai
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGZ110 (pFA6a-3FLAG-MNase-TRP1)
Plasmid#70233PurposeChEC-tagging vector encoding a 3xFLAG tag and aa 83-231 of Mnase in pFA6a-3HA-Trp1 replacing the 3xHA tag. These vectors retain compatibility with the F2/R1 primer pairs commonly used for epitope tagDepositorInsert3xFLAG tag and aa 83-231 of Mnase
UseYeast genomic targetingTagsExpressionMutationPromoterAvailable sinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-puro-PAX9
Plasmid#75079Purposeretroviral vector holding human PAX9 cDNADepositorInsertPAX9 (PAX9 Human)
UseRetroviralTagsExpressionMammalianMutationwild-type, full-length, without native 3' UTRPromoterAvailable sinceMay 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBT344_pattB-pCA-GFP-pA-FRT5
Plasmid#52550Purposefor Phic31 integrase-mediated transgene integration;the bacterial backbone can be subsequently removed by crossing to Flp-expressing miceDepositorInsertsPhiC31o attB (full length)
GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHR-PGK-LexA-CIB1-biLINuS-P2A-tagBFP
Plasmid#192609PurposeLentiviral vector for constitutive expression of LexA-CIB1-biLINuS-P2A-tagBFP.DepositorInsertLexA-CIB1-biLINuS-P2A-tagBFP
UseLentiviral and Synthetic BiologyTagstagBFPExpressionMutationPromoterPGKAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4L S382A
Plasmid#27036DepositorInsertNEDD4L (NEDD4L Human)
UseTagsHAExpressionMammalianMutationS382APromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only -
pFX-Venus-Sec61β
Plasmid#174467PurposeExpress Venus with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertVenus with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFX-mCherry-Sec61β
Plasmid#174468PurposeExpress mCherry with endoplasmic reticulum-targeting sequence in mammalian cellsDepositorInsertVenus with endoplasmic reticulum-targeting sequence
UseTagsSec61 subunit betaExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI HA NEDD4L S468A
Plasmid#27037DepositorInsertNEDD4L (NEDD4L Human)
UseTagsHAExpressionMammalianMutationS468APromoterAvailable sinceJan. 25, 2011AvailabilityAcademic Institutions and Nonprofits only