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Plasmid#197053PurposepUBC-NLS-HA-2xMCP-1xGFP-eRF3DepositorInsertpubc-nls-ha-MCP-GFP-eRF3
UseLentiviralTagsExpressionMammalianMutationPromoterubcAvailable sinceMarch 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
3xERRE/ERE-luciferase
Plasmid#37852DepositorInsert3 copies of ERRE/ERE upstream of luciferase
UseLuciferaseTagsExpressionMutationPromoterSV40Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
CD44P pGL3
Plasmid#19122DepositorInsertCD44 promoter (CD44 Human)
UseNon viral cloning vectorTagsExpressionMutationPromoterAvailable sinceSept. 3, 2008AvailabilityAcademic Institutions and Nonprofits only -
FRB-mCherry-Sec61β
Plasmid#186574PurposeExpression of FRB-mCherry-tagged ER protein Sec61beta in mammalian cellsDepositorInsertSEC61B (SEC61B Human)
UseTagsFRB-mCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUBQ10-GW
Plasmid#209370PurposeGateway cloning compatible binary vector for agrobacterium mediated transformation. MCS upstream of gateway, driven by UBQ10 promoterDepositorTypeEmpty backboneUseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJBEI-6409
Plasmid#47048PurposeBglBrick plasmid (=pBbA5c-MTSAe-T1f-MBI(f)-T1002i-Ptrc-trGPPS(co)-LS) coding for MEV pathway enzymes to produce limonene from glucose in E. coliDepositorInsertCodon-optimized sequences for MEV pathway expression in E. coli to produce limonene
UseSynthetic Biology; BglbrickTagsExpressionBacterialMutationPromoterPlacUV5Available sinceSept. 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pMK419 (GAL-OsTIR1(F74G))
Plasmid#140656PurposeGAL-OsTIR1(F74G)DepositorInsertGAL-OsTIR1(F74G)
UseTagsExpressionYeastMutationPromoterAvailable sinceNov. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hMYC
Plasmid#20723DepositorInsertc-MYC (MYC Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCH61 (multiAsCas12a-KRAB piggyBac)
Plasmid#217332PurposepiggyBac expression of multiAsCas12a-KRABDepositorInsertmultiAsCas12a
UseCRISPRTags6xMycNLS and HA-SV40NLS-XTEN80-KRAB-P2A-TagBFP2ExpressionMammalianMutationR1226A/E174R/S542R/K548RPromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAV-Syn-PSD95.FingR-eGFP-CCR5TC
Plasmid#125693PurposeGlobal labeling of excitatory synapses (green fluorescence)DepositorInsertPSD95.FingR-eGFP-CCR5TC
UseAAVTagsExpressionMammalianMutationPromoterSynapsinAvailable sinceJuly 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
mTFP1-N1
Plasmid#54521PurposeLocalization: N1 Cloning Vector, Excitation: 462, Emission: 492DepositorTypeEmpty backboneUseTagsmTFP1ExpressionMammalianMutationPromoterAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pODN-mNG-RHOA
Plasmid#183836PurposeRepair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.DepositorInsertRHOA homology arms with mNeonGreen-linker (RHOA Human)
UseCRISPR; Donor templateTagsmNeonGreen-linkerExpressionMammalianMutationHomology arms contain point mutations to remove t…PromoterAvailable sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
CMV-2mCh-PS
Plasmid#112268PurposeIDOCKS Acceptor ConstructDepositorInserttwo copies of mCherry followed by a pseudosubstrate peptide for PKC
UseTagsmChExpressionMammalianMutationPromoterCMVAvailable sinceNov. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-pmExRai-AKAR2
Plasmid#161755PurposePlasma membrane-targeted ExRai-AKAR2.DepositorInsertLyn-ExRai-AKAR2
UseTagsN-terminal targeting sequence from Lyn kinaseExpressionMammalianMutationPromoterCMVAvailable sinceNov. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
3XFLAG_GAPDH in pCW57_tTA_Blast
Plasmid#154211PurposeDox-off lentivirus construct all-in-one vector, with Blast resistance, for expression of GAPDHDepositorInsertGAPDH (GAPDH Human)
UseLentiviralTags3X FLAGExpressionMammalianMutationPromoterTRE repeats (dox off)Available sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pFB1_hMSH2
Plasmid#129423PurposeExpression human MSH2 protein using baculovirusDepositorInserthuman MSH2 (MSH2 Human)
UseTagsExpressionInsectMutationPromoterPolyhedrin promoterAvailable sinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pscALPSpuro-MmACE2 (Mouse)
Plasmid#158087PurposeExpresses mouse ACE2 in mammalian cellsDepositorInsertMouse ACE2 (Ace2 Mouse)
UseLentiviralTagsMycExpressionMammalianMutationPromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
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pGEX-4T1-14-3-3 epsilon GST
Plasmid#13279DepositorInsert14-3-3 epsilon (Ywhae Rat)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceOct. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX33
Plasmid#89632PurposeAllelic change mutagenesisDepositorInsertxylE
UseAllelic change mutagenesisTagsExpressionMutationPromoterPmycoAvailable sinceApril 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-3xFLAG-V5-ccdB
Plasmid#87064PurposeMammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tagDepositorTypeEmpty backboneUseTags3xFLAG-V5ExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF3432
Plasmid#144908PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNR3C1 (NR3C1 Human)
UseLentiviralTagsV5ExpressionMammalianMutationPromoterEF1aAvailable sinceFeb. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDV09_pCMV-ADAR1-p110
Plasmid#200404PurposeExpresses ADAR1 (p110 isoform) constitutively in mammalian cellsDepositorInsertADAR-p110
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH
Plasmid#180149PurposeThis plasmid is an empty vector used as the control for other plasmids which are in pT3-EF1aH backbone. It does not have any restriction site for cloning.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterEF1αAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
PE_CO
Plasmid#190335PurposePrime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cellsDepositorInsertPE2 with codon-optimized reverse transcriptase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Capri(1-10)
Plasmid#165142PurposeExpresses Capri(1-10) in mammalian cellsDepositorInsertCapri(1-10)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 8, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX459-puro-hGSDMD
Plasmid#185377PurposeFor mammalian expression of guide RNA: CGCGCCAGACGCGCCACCCT that targets human GSDMD (Gasdermin D)DepositorInsertGSDMD (GSDMD Human)
UseTagsExpressionMammalianMutationWTPromoterAvailable sinceJune 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSFFV_mNG3A(1-10)
Plasmid#157992PurposeLentiviral vector of constitutive expression of mNeonGreen3A(1-10)DepositorInsertpSFFV_mNG3K(1-10)
UseLentiviralTagsExpressionMammalianMutationW148L, M150V, K153M, N194K, F204LPromoterSFFVAvailable sinceOct. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
5xNF-kb_RE::RedF
Plasmid#124530PurposeNFKB RedFirefly luciferase reporterDepositorInsertNF-κβ RedF reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only